Optimization of medium formulation and seed conditions for expression of mature PsaA (pneumococcal surface adhesin A) in Escherichia coli using a sequential experimental design strategy and response surface methodology

Journal of Industrial Microbiology and Biotechnology

Volumn 39, Issue 6, 2012, Pages 897-908

  Larentis, Ariane Leites ^a   Quintal Nicolau, Júlia Fabiana Monteiro ^a   Argondizzo, Ana Paula Corrêa ^a   Galler, Ricardo ^a   Rodrigues, Maria Isabel ^b,c   Medeiros, Marco Alberto ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b UNIVERSITY OF CAMPINAS   (Brazil)

^c Campinas SP   (Brazil)

Author keywords

Design of experiments (DoE); Modeling; Optimization; Response surface methodology (RSM); Statistical experimental design

Indexed keywords

ADHESIN; BACTERIAL PROTEIN; GLUCOSE; GLYCEROL; KANAMYCIN; PNEUMOCOCCAL SURFACE ADHESIN A; POTASSIUM SALT; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; CELL GROWTH; CELL PH; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; DENSITOMETRY; ESCHERICHIA COLI; EXPERIMENTAL DESIGN; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; RESPONSE SURFACE METHOD; SEQUENTIAL EXPERIMENTAL DESIGN; YEAST;

ADHESINS, BACTERIAL; CULTURE MEDIA; ESCHERICHIA COLI; GENE EXPRESSION; GLYCEROL; LIPOPROTEINS; STREPTOCOCCUS PNEUMONIAE;

ANIMALIA; ESCHERICHIA COLI; STREPTOCOCCUS PNEUMONIAE;

EID: 84863481827     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-012-1099-8     Document Type: Article

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