메뉴 건너뛰기




Volumn 13, Issue 4, 2012, Pages 244-247

Improvement of PCR reaction conditions for site-directed mutagenesis of big plasmids

Author keywords

Mutant; Plasmid; Site directed mutagenesis (SDM)

Indexed keywords

ARTICLE; DNA MODIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

EID: 84863469161     PISSN: 16731581     EISSN: 18621783     Source Type: Journal    
DOI: 10.1631/jzus.B1100180     Document Type: Article
Times cited : (11)

References (16)
  • 1
    • 36249032614 scopus 로고    scopus 로고
    • A one-tube site-directed mutagenesis method using PCR and primer extension
    • DOI 10.1016/j.ab.2007.07.020, PII S0003269707004769
    • Chapnik, N., Sherman, H., Froy, O., 2008. A one-tube site-directed mutagenesis method using PCR and primer extension. Anal. Biochem., 372(2):255-257. [doi:10.1016/j.ab.2007.07.020] (Pubitemid 350123615)
    • (2008) Analytical Biochemistry , vol.372 , Issue.2 , pp. 255-257
    • Chapnik, N.1    Sherman, H.2    Froy, O.3
  • 2
    • 0001155699 scopus 로고
    • Introduction of a point mutation by sequential PCR steps
    • Cormack, B., 1994. Introduction of a point mutation by sequential PCR steps. Curr. Protoc. Mol. Biol., 2:8.5.7-8.5.9.
    • (1994) Curr. Protoc. Mol. Biol. , vol.2 , pp. 857-859
    • Cormack, B.1
  • 3
    • 67650865950 scopus 로고    scopus 로고
    • Simple and efficient site-directed mutagenesis using two singleprimer reactions in parallel to generate mutants for protein structure-function studies
    • doi:10.1186/1472-6750-9-61
    • Edelheit, O., Hanukoglu, A., Hanukoglu, I., 2009. Simple and efficient site-directed mutagenesis using two singleprimer reactions in parallel to generate mutants for protein structure-function studies. BMC Biotechnol., 9(1):61. [doi:10.1186/1472-6750-9-61]
    • (2009) BMC Biotechnol. , vol.9 , Issue.1 , pp. 61
    • Edelheit, O.1    Hanukoglu, A.2    Hanukoglu, I.3
  • 5
    • 70350724740 scopus 로고    scopus 로고
    • MALS: An efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR
    • doi:10. 1186/1472-6750-9-83
    • Fushan, A. A., Drayna, D. T., 2009. MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR. BMC Biotechnol., 9(1):83. [doi:10. 1186/1472-6750-9-83]
    • (2009) BMC Biotechnol. , vol.9 , Issue.1 , pp. 83
    • Fushan, A.A.1    Drayna, D.T.2
  • 7
    • 1842848033 scopus 로고    scopus 로고
    • Gene cloning and polymerase chain reaction with proliferating cell nuclear antigen from Thermococcus kodakaraensis KOD1
    • Kitabayashi, M., Nishiya, Y., Esaka, M., Itakura, M., Imanaka, T., 2002. Gene cloning and polymerase chain reaction with proliferating cell nuclear antigen from Thermococcus kodakaraensis KOD1. Biosci. Biotechnol. Biochem., 66(10):2194-2200. [doi:10.1271/bbb.66.2194] (Pubitemid 39251223)
    • (2002) Bioscience, Biotechnology and Biochemistry , vol.66 , Issue.10 , pp. 2194-2200
    • Kitabayashi, M.1    Nishiya, Y.2    Esaka, M.3    Itakura, M.4    Imanaka, T.5
  • 8
    • 42649089776 scopus 로고    scopus 로고
    • Single-step overlap-primerwalk polymerase chain reaction for multiple mutagenesis without overlap extension
    • doi:10.1016/j.ab.2008.03.003
    • Kumar, R., Rajagopal, K., 2008. Single-step overlap-primerwalk polymerase chain reaction for multiple mutagenesis without overlap extension. Anal. Biochem., 377(1):105-107. [doi:10.1016/j.ab.2008.03.003]
    • (2008) Anal. Biochem. , vol.377 , Issue.1 , pp. 105-107
    • Kumar, R.1    Rajagopal, K.2
  • 10
    • 80055111126 scopus 로고    scopus 로고
    • Homemade site directed mutagenesis of whole plasmids
    • doi:10.3791/1135
    • Laible, M., Boonrod, K., 2009. Homemade site directed mutagenesis of whole plasmids. J. Vis. Exp., (27): pii: 1135. [doi:10.3791/1135]
    • (2009) J. Vis. Exp. , Issue.27 , pp. 1135
    • Laible, M.1    Boonrod, K.2
  • 11
    • 37549056267 scopus 로고    scopus 로고
    • Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli
    • doi:10.1016/j.ab.2007.10.034
    • Li, J., Li, C., Xiao, W., Yuan, D., Wan, G., Ma, L., 2008. Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli. Anal. Biochem., 373(2):389-391. [doi:10.1016/j.ab.2007.10.034]
    • (2008) Anal. Biochem. , vol.373 , Issue.2 , pp. 389-391
    • Li, J.1    Li, C.2    Xiao, W.3    Yuan, D.4    Wan, G.5    Ma, L.6
  • 12
    • 58949085244 scopus 로고    scopus 로고
    • An efficient one-step sitedirected deletion, insertion, single and multiple-site plasmid mutagenesis protocol
    • doi:10.1186/1472-6750-8-91
    • Liu, H., Naismith, J. H., 2008. An efficient one-step sitedirected deletion, insertion, single and multiple-site plasmid mutagenesis protocol. BMC Biotechnol., 8(1):91. [doi:10.1186/1472-6750-8-91]
    • (2008) BMC Biotechnol. , vol.8 , Issue.1 , pp. 91
    • Naismith, J.H.1
  • 13
    • 0032750109 scopus 로고    scopus 로고
    • Characterization and application to hot start PCR of neutralizing monoclonal antibodies against KOD DNA polymerase
    • Mizuguchi, H., Nakatsuji, M., Fujiwara, S., Takagi, M., Imanaka, T., 1999. Characterization and application to Hot Start PCR of neutralizing monoclonal antibodies against KOD DNA polymerase. J. Biochem., 126(4):762-768. (Pubitemid 29500398)
    • (1999) Journal of Biochemistry , vol.126 , Issue.4 , pp. 762-768
    • Mizuguchi, H.1    Nakatsuji, M.2    Fujiwara, S.3    Takagi, M.4    Imanaka, T.5
  • 14
    • 40649107834 scopus 로고    scopus 로고
    • A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method
    • doi:10.1016/j.ab.2007.12.013
    • Tseng, W. C., Lin, J. W., Wei, T. Y., Fang, T. Y., 2008. A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method. Anal. Biochem., 375(2):376-378. [doi:10.1016/j.ab.2007.12.013]
    • (2008) Anal. Biochem. , vol.375 , Issue.2 , pp. 376-378
    • Tseng, W.C.1    Lin, J.W.2    Wei, T.Y.3    Fang, T.Y.4
  • 15
    • 66749177757 scopus 로고    scopus 로고
    • An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screeing
    • doi:10.1631/jzus. B0820367
    • Zhang, B. Z., Zhang, X., An, X. P., Ran, D. L., Zhou, Y. S., Lu, J., Tong, Y. G., 2009. An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screeing. J. Zhejiang Univ.-Sci. B, 10(6):479-482. [doi:10.1631/jzus. B0820367]
    • (2009) J. Zhejiang Univ.-Sci. B , vol.10 , Issue.6 , pp. 479-482
    • Zhang, B.Z.1    Zhang, X.2    An, X.P.3    Ran, D.L.4    Zhou, Y.S.5    Lu, J.6    Tong, Y.G.7
  • 16
    • 3843146246 scopus 로고    scopus 로고
    • An efficient one-step site-directed and site-saturation mutagenesis protocol
    • doi:10.1093/nar/gnh110
    • Zheng, L., Baumann, U., Reymond, J. L., 2004. An efficient one-step site-directed and site-saturation mutagenesis protocol. Nucleic Acids Res., 32(14):e115. [doi:10.1093/nar/gnh110]
    • (2004) Nucleic Acids Res. , vol.32 , Issue.14
    • Zheng, L.1    Baumann, U.2    Reymond, J.L.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.