MALS: An efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR

BMC Biotechnology

Volumn 9, Issue , 2009, Pages 83-

  Fushan, Alexey A ^a   Drayna, Dennis T ^a  

^a NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL REAGENTS; DNA AMPLIFICATION; DNA FRAGMENT; DNA MOLECULES; EFFICIENT STRATEGY; EXISTING METHOD; MULTIPLE MUTATIONS; MULTIPLE NUCLEOTIDES; PCR AMPLIFICATION; SHORT PERIODS; SITE DIRECTED MUTAGENESIS; SUBCLONING; TECHNICAL LIMITATIONS;

DNA; GENES; MUTAGENESIS; NUCLEIC ACIDS; OLIGONUCLEOTIDES; SPACE DIVISION MULTIPLE ACCESS; TARGETS;

AMPLIFICATION;

DNA FRAGMENT; GENOMIC DNA; OLIGONUCLEOTIDE;

ARTICLE; GENE AMPLIFICATION; GENE DELETION; GENE INSERTION; GENE MUTATION; GENE SEQUENCE; MOLECULAR CLONING; NUCLEIC ACID BASE SUBSTITUTION; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; LIGASE CHAIN REACTION; METHODOLOGY; MUTATION;

LIGASE CHAIN REACTION; MUTAGENESIS, SITE-DIRECTED; MUTATION; POLYMERASE CHAIN REACTION;

EID: 70350724740     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-83     Document Type: Article

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