An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening

Journal of Zhejiang University: Science B

Volumn 10, Issue 6, 2009, Pages 479-482

  Zhang, Bao Zhong ^a,b   Zhang, Xin ^a   An, Xiao Ping ^a   Ran, Duo Liang ^b   Zhou, Yu Sen ^a   Lu, Jun ^c   Tong, Yi Gang ^a  

^a BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^b XINJIANG AGRICULTURAL UNIVERSITY   (China)

^c CAPITAL MEDICAL UNIVERSITY   (China)

Author keywords

Mutant screening; Restriction endonuclease; Site directed mutagenesis (SDM)

Indexed keywords

DNA POLYMERASE; DOUBLE STRANDED DNA; PLASMID DNA; RESTRICTION ENDONUCLEASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; GENE TARGETING; INVERSE POLYMERASE CHAIN REACTION; MUTAGEN TESTING; NONHUMAN; POINT MUTATION; POLYMERIZATION; PRIORITY JOURNAL; RESTRICTION SITE; SEQUENCE ANALYSIS; SILENT GENE; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC SCREENING; MUTAGENESIS, SITE-DIRECTED; MUTATION; POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI;

EID: 66749177757     PISSN: 16731581     EISSN: 18621783     Source Type: Journal    
DOI: 10.1631/jzus.B0820367     Document Type: Article

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