Mesenchymal stem cell-conditioned medium facilitates angiogenesis and fracture healing in diabetic rats

Journal of Tissue Engineering and Regenerative Medicine

Volumn 6, Issue 7, 2012, Pages 559-569

  Wang, Chien Yuan ^a,b   Yang, Hsiao Bai ^b   Hsu, Han Shui ^a   Chen, Ling Lan ^c   Tsai, Chih Chien ^a,c   Tsai, Kuo Shu ^c   Yew, Tu Lai ^c   Kao, Yi Hsuan ^a   Hung, Shih Chieh ^a,b,c  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b Ton Yen General Hospital   (Taiwan)

^c TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

Author keywords

Angiogenesis; Bone healing; Conditioned medium; Gelatin sponge; Hypoxic culture; Mesenchymal stem cell

Indexed keywords

BONE; COMPUTERIZED TOMOGRAPHY; ENDOTHELIAL CELLS; FRACTURE; STEM CELLS;

ANGIOGENESIS; BONE HEALING; CONDITIONED MEDIUM; DIABETIC RATS; FRACTURE HEALING; GELATIN SPONGE; HYPOXIC CULTURE; MEDIA GROUPS; MESENCHYMAL STEM CELL; MINIMAL ESSENTIAL MEDIUMS;

CELL CULTURE;

CD31 ANTIGEN; GELATIN SPONGE;

ANGIOGENESIS; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BONE DENSITY; BONE GROWTH; CELL COUNT; CELL CULTURE; CELL STRAIN HEK293; CONTROLLED STUDY; CULTURE MEDIUM; DIABETES MELLITUS; ENDOTHELIUM CELL; FRACTURE HEALING; FRACTURE NONUNION; HISTOLOGY; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MALE; MESENCHYMAL STEM CELL; MESENCHYMAL STEM CELL DERIVED CONDITIONED MEDIUM; MESENCHYMAL STEM CELL TRANSPLANTATION; MICRO-COMPUTED TOMOGRAPHY; MINIMAL ESSENTIAL MEDIUM; NONHUMAN; PRIORITY JOURNAL; RAT;

EID: 84863453169     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.461     Document Type: Article

References (33)

1. Carano RA, Filvaroff EH. 2003; Angiogenesis and bone repair. Drug Discov Today 8(21): 980-989.
2. Chen L, Tredget EE, Wu PYG, et al. 2008; Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound healing. PLoS One 3(4): e1886.
3. Crisostomo PR, Wang M, Markel TA, et al. 2007; Stem cell mechanisms and paracrine effects: potential in cardiac surgery. Shock 28(4): 375.
4. Dharmasaroja P. 2009; Bone marrow-derived mesenchymal stem cells for the treatment of ischemic stroke. J Clin Neurosci 16(1): 12-20.
5. Duarte JM, Oliveira CR, Ambrosio AF, et al. 2006; Modification of adenosine a1 and a2a receptor density in the hippocampus of streptozotocin-induced diabetic rats. Neurochem Int 48(2): 144-150.
6. Gandhi A, Beam HA, O'Connor JP, et al. 2005; The effects of local insulin delivery on diabetic fracture healing. Bone 37(4): 482-490.
7. Hausman MR, Schaffler MB, Majeska RJ. 2001; Prevention of fracture healing in rats by an inhibitor of angiogenesis. Bone 29(6): 560-564.
8. Hayashi T, Abe K, Itoyama Y. 1998; Reduction of ischemic damage by application of vascular endothelial growth factor in rat brain after transient ischemia. J Cereb Blood Flow Metab 18(8): 887-895.
9. Herskind AM, Christensen K, Norgaard-Andersen K, et al. 1992; Diabetes mellitus and healing of closed fractures. Diabetes Metab 18(1): 63-64.
10. Hung SC, Pochampally RR, Chen SC, et al. 2007; Angiogenic effects of human multipotent stromal cell conditioned medium activate the pi3k-akt pathway in hypoxic endothelial cells to inhibit apoptosis, increase survival, and stimulate angiogenesis. Stem Cells 25(9): 2363-2370.
11. Kawamura K, Yajima H, Ohgushi H, et al. 2006; Experimental study of vascularized tissue-engineered bone grafts. Plast Reconstr Surg 117(5): 1471-1479.
12. Kinnaird T, Stabile E, Burnett MS, et al. 2004; Marrow-derived stromal cells express genes encoding a broad spectrum of arteriogenic cytokines and promote in vitro and in vivo arteriogenesis through paracrine mechanisms. Circ Res 94(5): 678-685.
13. Lu H, Kraut D, Gerstenfeld LC, et al. 2003; Diabetes interferes with the bone formation by affecting the expression of transcription factors that regulate osteoblast differentiation. Endocrinology 144(1): 346-352.
14. Murashita T, Nakayama Y, Hirano T, et al. 1996; Acceleration of granulation tissue ingrowth by hyaluronic acid in artificial skin. Br J Plast Surg 49(1): 58-63.
15. Nakasa T, Ishida O, Sunagawa T, et al. 2005; Prefabrication of vascularized bone graft using a combination of fibroblast growth factor-2 and vascular bundle implantation into a novel interconnected porous calcium hydroxyapatite ceramic. J Biomed Mater Res A 75(2): 350-355.
16. Nishida J, Shimamura T. 2008; Methods of reconstruction for bone defect after tumor excision: a review of alternatives. Med Sci Monit 14(8): RA107-RA113.
17. Ogasawara A, Nakajima A, Nakajima F, et al. 2008; Molecular basis for affected cartilage formation and bone union in fracture healing of the streptozotocin-induced diabetic rat. Bone 43(5): 832-839.
18. Phinney DG, Kopen G, Righter W, et al. 1999; Donor variation in the growth properties and osteogenic potential of human marrow stromal cells. J Cell Biochem 75(3): 424-436.
19. Rubatt JM, Darcy KM, Hutson A, et al. 2009; Independent prognostic relevance of microvessel density in advanced epithelial ovarian cancer and associations between cd31, cd105, p53 status, and angiogenic marker expression: a gynecologic oncology group study. Gynecol Oncol 112(3): 469-474.
20. Sekiya I, Larson BL, Smith JR, et al. 2002; Expansion of human adult stem cells from bone marrow stroma: conditions that maximize the yields of early progenitors and evaluate their quality. Stem Cells 20(6): 530-541.
21. Schlueter C, Weber H, Meyer B, et al. 2005; Angiogenetic signaling through hypoxia: Hmgb1: an angiogenetic switch molecule. Am J Pathol 166(4): 1259-1263.
22. Sung HJ, Sakala Labazzo KM, Bolikal D, et al. 2008; Angiogenic competency of biodegradable hydrogels fabricated from polyethylene glycol-crosslinked tyrosine-derived polycarbonates. Eur Cell Mater 15: 77-87.
23. Takahashi Y, Yamamoto M, Tabata Y. 2005; Enhanced osteoinduction by controlled release of bone morphogenetic protein-2 from biodegradable sponge composed of gelatin and β-tricalcium phosphate. Biomaterials 26(23): 4856-4865.
24. Tang YL, Zhao Q, Qin X, et al. 2005; Paracrine action enhances the effects of autologous mesenchymal stem cell transplantation on vascular regeneration in rat model of myocardial infarction. Ann Thorac Surg 80(1): 229-236; discussion, 236-237.
25. Timmers L, Lim SK, Arslan F, et al. 2007; Reduction of myocardial infarct size by human mesenchymal stem cell conditioned medium. Stem Cell Res 1(2): 129-137.
26. Togel F, Hu Z, Weiss K, et al. 2005; Administered mesenchymal stem cells protect against ischemic acute renal failure through differentiation-independent mechanisms. Am J Physiol Renal Physiol 289(1): F31.
27. Tsai CC, Chen YJ, Yew TL, et al. 2011; Hypoxia inhibits senescence and maintains mesenchymal stem cell properties through down-regulation of E2A-P21 by HIF-TWIST. Blood 117(2): 459-469.
28. Tsiridis E, Upadhyay N, Giannoudis P. 2007; Molecular aspects of fracture healing: which are the important molecules? Injury 38(suppl 1): S11-S25.
29. Uchida S, Sakai A, Kudo H, et al. 2003; Vascular endothelial growth factor is expressed along with its receptors during the healing process of bone and bone marrow after drill-hole injury in rats. Bone 32(5): 491-501.
30. van Tienen TG, Heijkants RG, Buma P, et al. 2002; Tissue ingrowth and degradation of two biodegradable porous polymers with different porosities and pore sizes. Biomaterials 23(8): 1731-1738.
31. Yang X, Ricciardi BF, Hernandez-Soria A, et al. 2007; Callus mineralization and maturation are delayed during fracture healing in interleukin-6 knockout mice. Bone 41(6): 928-936.
32. Zhang ZY, Teoh SH, Chong MS, et al. 2010; Neo-vascularization and bone formation mediated by fetal mesenchymal stem cell tissue-engineered bone grafts in critical-size femoral defects. Biomaterials 31(4): 608-620.
33. Zhou J, Lin H, Fang T, et al. 2010; The repair of large segmental bone defects in the rabbit with vascularized tissue engineered bone. Biomaterials 31(6): 1171-1179.