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Volumn 847, Issue , 2012, Pages 35-47

Genetic transformation of the model green alga Chlamydomonas reinhardtii

Author keywords

Autolysin; Biolistic transformation; Chlamydomonas reinhardtii; Expression strain; Green alga; Luciferase activity assay; Particle gun; Reporter gene; Selectable marker gene; Transformation

Indexed keywords

ARTICLE; CHLAMYDOMONAS REINHARDTII; GENE TRANSFER; GENE VECTOR; GENETIC MARKER; GENETIC TRANSFORMATION; GENETICS; REPORTER GENE; TRANSGENIC PLANT;

EID: 84863350333     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-558-9_4     Document Type: Article
Times cited : (46)

References (30)
  • 2
    • 25444488729 scopus 로고    scopus 로고
    • Portrait of a species: Chlamydomonas reinhardtii
    • Pröschold, T., Harris, E. H., and Coleman, A. W. (2005) Portrait of a species: Chlamydomonas reinhardtii . Genetics 170 , 1601-1610.
    • (2005) Genetics , vol.170 , pp. 1601-1610
    • Pröschold, T.1    Harris, E.H.2    Coleman, A.W.3
  • 3
    • 0032487367 scopus 로고    scopus 로고
    • Chlamydomonas genetics, a tool for the study of bioenergetic pathways
    • Hippler, M., Redding, K., and Rochaix, J.-D. (1998) Chlamydomonas genetics, a tool for the study of bioenergetic pathways. Biochim. Biophys. Acta 1367 , 1-62.
    • (1998) Biochim. Biophys. Acta , vol.1367 , pp. 1-62
    • Hippler, M.1    Redding, K.2    Rochaix, J.-D.3
  • 4
    • 0026035050 scopus 로고
    • Engineering the chloroplast genome: Techniques and capabilities for chloroplast transformation in chlamydomonas reinhardtii
    • Kindle, K. L., Richards, K. L., and Stern, D. B. (1991) Engineering the chloroplast genome: Techniques and capabilities for chloroplast transformation in Chlamydomonas reinhardtii . Proc. Natl. Acad. Sci. USA 88 , 1721-1725.
    • (1991) Proc. Natl. Acad. Sci. USA , vol.88 , pp. 1721-1725
    • Kindle, K.L.1    Richards, K.L.2    Stern, D.B.3
  • 5
    • 33645218188 scopus 로고    scopus 로고
    • Higheffi ciency biolistic transformation of chlamydomonas mitochondria can be used to insert mutations in complex i genes
    • Remacle, C., Cardol, P., Coosemans, N., Gaisne, M., and Bonnefoy, N. (2006) Higheffi ciency biolistic transformation of Chlamydomonas mitochondria can be used to insert mutations in complex I genes. Proc. Natl. Acad. Sci. USA 103 , 4771-4776.
    • (2006) Proc. Natl. Acad. Sci. USA , vol.103 , pp. 4771-4776
    • Remacle, C.1    Cardol, P.2    Coosemans, N.3    Gaisne, M.4    Bonnefoy, N.5
  • 6
    • 0025117612 scopus 로고
    • High-frequency nuclear transformation of chlamydomonas reinhardtii
    • Kindle, K. L. (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii . Proc. Natl. Acad. Sci. USA 87 , 1228-1232.
    • (1990) Proc. Natl. Acad. Sci. USA , vol.87 , pp. 1228-1232
    • Kindle, K.L.1
  • 8
    • 19044380672 scopus 로고    scopus 로고
    • Functional genomics of eukaryotic photosynthesis using insertional mutagenesis of chlamydomonas reinhardtii
    • Dent, R. M., Haglund, C. M., Chin, B. L., Kobayashi, M. C., and Niyogi, K. K. (2005) Functional genomics of eukaryotic photosynthesis using insertional mutagenesis of Chlamydomonas reinhardtii . Plant Physiol. 137 , 545-556.
    • (2005) Plant Physiol. , vol.137 , pp. 545-556
    • Dent, R.M.1    Haglund, C.M.2    Chin, B.L.3    Kobayashi, M.C.4    Niyogi, K.K.5
  • 9
    • 8444222059 scopus 로고    scopus 로고
    • Tandem inverted repeat system for selection of effective transgenic RNAi strains in chlamydomonas
    • Rohr, J., Sarkar, N., Balenger, S., Jeong, B.-r, and Cerutti, H. (2004) Tandem inverted repeat system for selection of effective transgenic RNAi strains in Chlamydomonas . Plant J. 40 , 611-621.
    • (2004) Plant J. , vol.40 , pp. 611-621
    • Rohr, J.1    Sarkar, N.2    Balenger, S.3    Jeong, B.-R.4    Cerutti, H.5
  • 10
    • 63349090914 scopus 로고    scopus 로고
    • Highly specifi c gene silencing by artifi cial microRNAs in the unicellular alga chlamydomonas reinhardtii
    • Molnar, A., Bassett, A., Thuenemann, E., Schwach, F., Karkare, S., Ossowski, S., Weigel, D., and Baulcombe, D. (2009) Highly specifi c gene silencing by artifi cial microRNAs in the unicellular alga Chlamydomonas reinhardtii . Plant J. 58 , 165-174.
    • (2009) Plant J. , vol.58 , pp. 165-174
    • Molnar, A.1    Bassett, A.2    Thuenemann, E.3    Schwach, F.4    Karkare, S.5    Ossowski, S.6    Weigel, D.7    Baulcombe, D.8
  • 11
    • 63349106223 scopus 로고    scopus 로고
    • Gene silencing by artifi cial micro RNAs in chlamydomonas
    • Zhao, T., Wang, W., Bai, X., and Qi, Y. (2009) Gene silencing by artifi cial micro RNAs in Chlamydomonas . Plant J. 58 , 157-164.
    • (2009) Plant J. , vol.58 , pp. 157-164
    • Zhao, T.1    Wang, W.2    Bai, X.3    Qi, Y.4
  • 12
    • 33644779653 scopus 로고    scopus 로고
    • Dissecting the molecular mechanisms of intrafl agellar transport in chlamydomonas
    • Pedersen, L. B., Geimer, S., and Rosenbaum, J. L. (2006) Dissecting the molecular mechanisms of intrafl agellar transport in Chlamydomonas . Curr. Biol. 16 , 450-459.
    • (2006) Curr. Biol. , Issue.16 , pp. 450-459
    • Pedersen, L.B.1    Geimer, S.2    Rosenbaum, J.L.3
  • 16
    • 0033179996 scopus 로고    scopus 로고
    • A synthetic gene coding for the green fl uorescent protein (GFP) is a versatile reporter in chlamydomonas reinhardtii
    • Fuhrmann, M., Oertel, W., and Hegemann, P. (1999) A synthetic gene coding for the green fl uorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii . Plant J. 19 , 353-361.
    • (1999) Plant J. , vol.19 , pp. 353-361
    • Fuhrmann, M.1    Oertel, W.2    Hegemann, P.3
  • 17
    • 0342680049 scopus 로고    scopus 로고
    • The HSP70A promoter as a tool for the improved expression of transgenes in chlamydomonas
    • Schroda, M., Blöcker, D., and Beck, C. F. (2000) The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas . Plant J. 21 , 121-131.
    • (2000) Plant J. , vol.21 , pp. 121-131
    • Schroda, M.1    Blöcker, D.2    Beck, C.F.3
  • 18
  • 19
    • 0035904396 scopus 로고    scopus 로고
    • A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to chlamydomonas reinhardtii
    • Sizova, I., Fuhrmann, M., and Hegemann, P. (2001) A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii . Gene 277 , 221-229.
    • (2001) Gene , vol.277 , pp. 221-229
    • Sizova, I.1    Fuhrmann, M.2    Hegemann, P.3
  • 20
    • 0028279252 scopus 로고
    • The CRY1 gene in chlamydomonas reinhardtii : Structure and use as a dominant selectable marker for nuclear transformation
    • Nelson, J. A. E., Savereide, P. B., and Lefebvre, P. A. (1994) The CRY1 gene in Chlamydomonas reinhardtii : Structure and use as a dominant selectable marker for nuclear transformation. Mol. Cell. Biol. 14 , 4011-4019.
    • (1994) Mol. Cell. Biol. , vol.14 , pp. 4011-4019
    • Nelson, J.A.E.1    Savereide, P.B.2    Lefebvre, P.A.3
  • 21
    • 0031830846 scopus 로고    scopus 로고
    • Effi cient foreign gene expression in chlamydomonas reinhardtii mediated by an endogenous intron
    • Lumbreras, V., Stevens, D. R., and Purton, S. (1998) Effi cient foreign gene expression in Chlamydomonas reinhardtii mediated by an endogenous intron. Plant J. 14 , 441-447.
    • (1998) Plant J. , vol.14 , pp. 441-447
    • Lumbreras, V.1    Stevens, D.R.2    Purton, S.3
  • 22
    • 0036967786 scopus 로고    scopus 로고
    • An engineered Streptomyces hygroscopicus aph 7 gene mediates dominant resistance against hygromycin B in chlamydomonas reinhardtii
    • Berthold, P., Schmitt, R., and Mages, W. (2002) An engineered Streptomyces hygroscopicus aph 7" gene mediates dominant resistance against hygromycin B in Chlamydomonas reinhardtii . Protist 153 , 401-412.
    • (2002) Protist , vol.153 , pp. 401-412
    • Berthold, P.1    Schmitt, R.2    Mages, W.3
  • 23
    • 0033026887 scopus 로고    scopus 로고
    • The argininosuccinate lyase gene of chlamydomonas reinhardtii : Cloning of the cDNA and its characterization as a selectable shuttle marker
    • Auchincloss, A. H., Loroch, A. I., and Rochaix, J.-D. (1999) The argininosuccinate lyase gene of Chlamydomonas reinhardtii : cloning of the cDNA and its characterization as a selectable shuttle marker. Mol. Gen. Genet. 261 , 21-30.
    • (1999) Mol. Gen. Genet. , vol.261 , pp. 21-30
    • Auchincloss, A.H.1    Loroch, A.I.2    Rochaix, J.-D.3
  • 24
    • 71249119635 scopus 로고    scopus 로고
    • The Chlamydomonas chloroplast HLP protein is required for nucleoid organization and genome maintenance
    • Karcher, D., Köster, D., Schadach, A., Klevesath, A., and Bock, R. (2009) The Chlamydomonas chloroplast HLP protein is required for nucleoid organization and genome maintenance. Mol. Plant 2 , 1223-1232.
    • (2009) Mol. Plant , vol.2 , pp. 1223-1232
    • Karcher, D.1    Köster, D.2    Schadach, A.3    Klevesath, A.4    Bock, R.5
  • 25
    • 62149107922 scopus 로고    scopus 로고
    • Generation of Chlamydomonas strains that effi - Ciently express nuclear transgenes
    • Neupert, J., Karcher, D., and Bock, R. (2009) Generation of Chlamydomonas strains that effi - ciently express nuclear transgenes. Plant J. 57 , 1140-1150.
    • (2009) Plant J. , vol.57 , pp. 1140-1150
    • Neupert, J.1    Karcher, D.2    Bock, R.3
  • 26
    • 14644445240 scopus 로고    scopus 로고
    • Monitoring dynamic expression of nuclear genes in chlamydomonas reinhardtii by using a synthetic luciferase reporter gene
    • Fuhrmann, M., Hausherr, A., Ferbitz, L., Schödl, T., Heitzer, M., and Hegemann, P. (2004) Monitoring dynamic expression of nuclear genes in Chlamydomonas reinhardtii by using a synthetic luciferase reporter gene. Plant Mol. Biol. 55 , 869-881.
    • (2004) Plant Mol. Biol. , vol.55 , pp. 869-881
    • Fuhrmann, M.1    Hausherr, A.2    Ferbitz, L.3    Schödl, T.4    Heitzer, M.5    Hegemann, P.6
  • 27
    • 44349192711 scopus 로고    scopus 로고
    • A codon-optimized luciferase from gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga chlamydomonas reinhardtii
    • Shao, N. and Bock, R. (2008) A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii . Curr. Genet. 53 , 381-388.
    • (2008) Curr. Genet. , vol.53 , pp. 381-388
    • Shao, N.1    Bock, R.2
  • 28
    • 0034888190 scopus 로고    scopus 로고
    • The fl anking regions of PsaD drive effi cient gene expression in the nucleus of the green alga chlamydomonas reinhardtii
    • Fischer, N. and Rochaix, J.-D. (2001) The fl anking regions of PsaD drive effi cient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii . Mol. Genet. Genom. 265 , 888-894.
    • (2001) Mol. Genet. Genom. , vol.265 , pp. 888-894
    • Fischer, N.1    Rochaix, J.-D.2
  • 29
    • 0001727264 scopus 로고
    • Cellular fractionation of chlamydomonas reinhardtii with emphasis on the isolation of the chloroplast
    • Klein, U., Chen, C., Gibbs, M. and Platt-Aloia, K. A. (1983) Cellular fractionation of Chlamydomonas reinhardtii with emphasis on the isolation of the chloroplast. Plant Physiol. 72 , 481-487.
    • (1983) Plant Physiol. , vol.72 , pp. 481-487
    • Klein, U.1    Chen, C.2    Gibbs, M.3    Platt-Aloia, K.A.4
  • 30
    • 3142689994 scopus 로고    scopus 로고
    • Studying RNA editing in transgenic chloroplasts of higher plants
    • Bock, R. (2004) Studying RNA editing in transgenic chloroplasts of higher plants. Methods Mol. Biol. 265 , 345-356.
    • (2004) Methods Mol. Biol. , vol.265 , pp. 345-356
    • Bock, R.1


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