Mutations affecting the activity of the cyclodextrin glucanotransferase of Paenibacillus pabuli US132: Insights into the low hydrolytic activity of cyclodextrin glucanotransferases

Biologia

Volumn 67, Issue 4, 2012, Pages 636-643

  Jemli, Sonia ^a   Ben Ali, Mamdouh ^a   Ben Hlima, Hajer ^a   Khemakhem, Bassem ^a   Bejar, Samir ^a  

^a UNIVERSITY OF SFAX   (Tunisia)

Author keywords

cyclization; cyclodextrin glucanotransferase; homology modeling; hydrolysis; site directed mutagenesis; structural barrier

Indexed keywords

PAENIBACILLUS; PAENIBACILLUS PABULI;

EID: 84863309162     PISSN: 00063088     EISSN: 13369563     Source Type: Journal    
DOI: 10.2478/s11756-012-0055-4     Document Type: Article

References (35)

1. Beier L., Svendsen A., Andersen C., Frandsen T. P., Borchert T. V. & Cherry J. R. 2000. Conversion of the maltogenic alphaamylase Novamyl into a CGTase. Protein Eng. 13: 509-513.
2. Ben Messaoud E., Ben Ali M., Elleuch N., Fourati Masmoudi N. & Bejar S. 2004. Purification and properties of a maltoheptaose and maltohexaose-forming amylase produced by Bacillus subtilis US116. Enzyme Microb. Technol. 34: 662-666.
3. Bradford M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
4. Dauter Z., Dauter M., Brzozowski A. M., Christensen S., Borchert T. V., Beier L., Wilson K. S. & Davies G. J. 1999. X-ray structure of Novamyl, the five-domain "maltogenic" α-amylase from Bacillus stearothermophilus: maltose and acarbose complexes at 1. 7 Å resolution. Biochemistry 38: 8385-8392.
5. Goesaert H., Slade L., Levine H. & Delcour J. A. 2009. Amylases and bread firming - an integrated view. J. Cereal Sci. 50: 345-353.
6. Henrissat B. & Davies G. J. 1997. Structural and sequence-based classification of glycoside hydrolases. Curr. Opin. Struct. Biol. 7: 637-644.
7. Janecek S., MacGregor E. A. & Svensson B. 1995. Characteristic differences in the primary structure allow discrimination of cyclodextrin glucanotransferases from α-amylases. Biochem. J. 305: 685-686.
8. Janecek S., Svensson B. & MacGregor E. A. 2003. Relation between domain evolution, specificity, and taxonomy of the α-amylase family members containing a C-terminal starchbinding domain. Eur. J. Biochem. 270: 635-645.
9. Jemli S., Ben Messaoud E., Ayadi-Zouari D., Naili B., Khemakhem B. & Bejar S. 2007. A β-cyclodextrin glycosyltransferase from a newly isolated Paenibacillus pabuli US132 strain: purification, properties and potential use in breadmaking. Biochem. Eng. J. 34: 44-50.
10. Jemli S., Ben Messaoud E., Ben Mabrouk S. & Bejar S. 2008. The cyclodextrin glycosyltransferase of Paenibacillus pabuli US132 strain: molecular characterization and overproduction of the recombinant enzyme. J. Biomed. Biotechnol. Article ID 692573, DOI: 10. 1155/2008/692573.
11. Kelly R. M., Dijkhuizen L. & Leemhuis H. 2009. The evolution of cyclodextrin glucanotransferase product specificity. Appl. Microbiol. Biotechnol. 84: 119-133.
12. Laemmli U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
13. Lee S. H., Kim Y. W., Lee S., Auh J. H., Yoo S. S., Kim T. J., Kim J. W., Kim S. T., Rho H. J., Choi J. H., Kim Y. B. & Park K. H. 2002. Modulation of cyclizing activity and thermostability of cyclodextrin glucanotransferase and its application as an antistaling enzyme. J. Agric. Food Chem. 50: 1411-1415.
14. Leemhuis H., Dijkstra B. W. & Dijkhuizen L. 2002a. Mutations converting cyclodextrin glycosyltransferase from a transglycosylase into a starch hydrolase. FEBS Lett. 514: 189-192.
15. Leemhuis H., Dijkstra B. W. & Dijkhuizen L. 2003a. Thermoanaerobacterium thermosulfurigenes cyclodextrin glycosyltransferase. Mechanism and kinetics of inhibition by acarbose and cyclodextrins. Eur. J. Biochem. 270: 155-162.
16. Leemhuis H., Kelly R. M. & Dijkhuizen L. 2010. Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications. Appl. Microbiol. Biotechnol. 85: 823-835.
17. Leemhuis H., Kragh K. M., Dijkstra B. W. & Dijkhuizen L. 2003b. Engineering cyclodextrin glycosyltransferase into a starch hydrolase with a high exo-specificity. J. Biotechnol. 103: 203-212.
18. Leemhuis H., Uitdehaag J. C. M., Rozeboom H. J., Dijkstra B. W. & Dijkhuizen L. 2002b. The remote substrate binding subsite -6 in cyclodextrin-glycosyltransferase controls the transferase activity of the enzyme via an induced-fit mechanism. J. Biol. Chem. 277: 1113-1119.
19. Li Z., Zhang J., Wang M., Gu Z., Du G., Li J., Wu J. & Chen J. 2009. Mutations at subsite -3 in cyclodextrin glycosyltransferase from Paenibacillus macerans enhancing α-cyclodextrin specificity. Appl. Microbiol. Biotechnol. 83: 483-490.
20. MacGregor E. A., Janecek S. & Svensson B. 2001. Relationship of sequence and structure to specificity in the α-amylase family of enzymes. Biochim. Biophys. Acta 146: 1-20.
21. Martin Del Valle E. M. 2004. Cyclodextrins and their uses: a review. Process Biochem. 39: 1033-1046.
22. Mutsaers J. H. G. M. & van Eijk J. H. 1999. Process for increasing the volume of a baked product. US Patent 5916607.
23. Norman B. E., & Jørgensen S. T. 1992. Thermoanaerobacter sp. CGTase: its properties and application. Denpun Kagaku, 39: 101-108.
24. Ohdan K., Kuriki T., Takata H., Kaneko H. & Okada S. 2000. Introduction of raw starch-binding domains into Bacillus subtilis α-amylase by fusion with the starch-binding domain of Bacillus cyclomaltodextrin glucanotransferase. Appl. Environ. Microbiol. 66: 3058-3064.
25. Olesen T. 1991. Anti-staling process and agent. Patent application WO 9104669.
26. Parsiegla G., Schmidt A. K. & Schulz G. E. 1998. Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the γ-cyclodextrin production. Eur. J. Biochem. 255: 710-717.
27. Penninga D., Strokopytov B., Rozeboom H. J., Lawson C. L., Dijkstra B. W., Bergsma J. & Dijkhuizen L. 1995. Site-directed mutations in tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 affect activity and product specificity. Biochemistry 34: 3368-3376.
28. Shim J. H., Kim Y. W., Kim T. J., Chae H. Y., Park J. H., Cha H., Kim J. W., Kim Y. R., Schaefer T., Spendler T. Moon T. W. & Park K. H. 2004. Improvement of cyclodextrin glucanotransferase as an antistaling enzyme by error-prone PCR. Protein Eng. 17: 205-211.
29. Somogyi M. 1952. Notes on sugar determination. J. Biol. Chem. 195: 19-23.
30. Sun T., Jiang B. & Pan B. 2011. Microwave accelerated transglycosylation of rutin by cyclodextrin glucanotransferase from Bacillus sp. SK13. 002. Int. J. Mol. Sci. 12: 3786-3796.
31. Svensson B., Tovborg Jensen M., Mori H., Sass Bak-Jensen K., Bønsager B., Nielsen P. K., Kramhøft B., Prćtorius-Ibba M., Nør J. & Juge N. 2002. Fascinating facets of function and structure of amylolytic enzymes of glycoside hydrolase family 13. Biologia 57(Suppl. 11): 5-19.
32. van der Veen B. A., Uitdehaag J. C., Dijkstra B. W. & Dijkhuizen L. 2000. The role of arginine 47 in the cyclization and coupling reactions of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 Implications for product inhibition and product specificity. Eur. J. Biochem. 267: 3432-3441.
33. Volkova D. A., Lopatin S. A. & Varlamov V. P. 2000. One-step affinity purification of cyclodextrin glucanotransferase from Bacillus sp. 1070. Moscow Univ. Chem. Bull. 41(Suppl.): 67-69.
34. Wind R. D., Liebl W., Buitelaar R. M., Penninga D., Spreinat A., Dijkhuizen L. & Bahl H. 1995. Cyclodextrin formation by the thermostable α-amylase of Thermoanaerobacterium thermosulfurigenes EM1 and reclassification of the enzyme as a cyclodextrin glycosyltransferase. Appl. Environ. Microbiol. 61: 1257-1265.
35. Zouari Ayadi D., Kammoun R., Jemli S. & Bejar S. 2011. Excretory overexpression of Paenibacillus pabuli US132 cyclodextrin glucanotransferase (CGTase) in Escherichia coli: gene cloning and optimization of the culture conditions using experimental design. Biologia 66: 945-953.