Recombinant production of self-assembling β-structured peptides using SUMO as a fusion partner

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Prakash, Abhinav ^a   Parsons, Stephen J ^a   Kyle, Stuart ^a   McPherson, Michael J ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

Hydrogel; Peptide; Recombinant expression; Scaffold; Self assembly

Indexed keywords

HYBRID PROTEIN; SUMO PROTEIN; WATER;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CIRCULAR DICHROISM; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE; TRANSMISSION ELECTRON MICROSCOPY;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; ESCHERICHIA COLI; GENETIC VECTORS; HYDROGEL; PEPTIDES; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84863192444     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-92     Document Type: Article

References (30)

1. Webber MJ, Tongers J, Newcomb CJ, Marquardt KT, Bauersachs J, Losordo DW, Stupp SI. Supramolecular nanostructures that mimic VEGF as a strategy for ischemic tissue repair. Proc Natl Acad Sci USA 2011, 108:13438-13443. 10.1073/pnas.1016546108, 3158182, 21808036.
2. Cho H, Balaji S, Sheikh AQ, Hurley JR, Tian YF, Collier JH, Crombleholme TM, Narmoneva DA. Regulation of endothelial cell activation and angiogenesis by injectable peptide nanofibers. Acta Biomater 2012, 8:154-164. 10.1016/j.actbio.2011.08.029, 21925628.
3. Gelain F, Unsworth LD, Zhang S. Slow and sustained release of active cytokines from self-assembling peptide scaffolds. J Control Release 2010, 145:231-239. 10.1016/j.jconrel.2010.04.026, 20447427.
4. Bell CJ, Carrick LM, Katta J, Jin Z, Ingham E, Aggeli A, Boden N, Waigh TA, Fisher J. Self-assembling peptides as injectable lubricants for osteoarthritis. J Biomed Mater Res A 2006, 78:236-246.
5. Firth A, Aggeli A, Burke JL, Yang XB, Kirkham J. Biomimetic self-assembling peptides as injectable scaffolds for hard tissue engineering. Nanomedicine 2006, 1:189-199.
6. Kirkham J, Firth A, Vernals D, Boden N, Robinson C, Shore RC, Brookes SJ, Aggeli A. Self-assembling peptide scaffolds promote enamel remineralization. J Dent Res 2007, 86:426-430. 10.1177/154405910708600507, 17452562.
7. Aggeli A, Bell M, Boden N, Keen JN, Knowles PF, McLeish TCB, Pitkeathly M, Radford SE. Responsive gels formed by the spontaneous self-assembly of peptides into polymeric beta-sheet tapes. Nature 1997, 386:259-262. 10.1038/386259a0, 9069283.
8. Aggeli A, Bell M, Boden N, Carrick LM, Strong AE. Self-assembling peptide polyelectrolyte beta-sheet complexes form nematic hydrogels. Angew Chemie Int Ed Engl 2003, 42:5603-5606.
9. Aggeli A, Nyrkova IA, Bell M, Harding R, Carrick L, McLeish TCB, Semenov AN, Boden N. Hierarchical self-assembly of chiral rod-like molecules as a model for peptide beta-sheet tapes, ribbons, fibrils, and fibers. Proc Natl Acad Sci USA 2001, 98:11857-11862. 10.1073/pnas.191250198, 59814, 11592996.
10. Kyle S, Aggeli A, Ingham E, McPherson MJ. Recombinant self-assembling peptides as biomaterials for tissue engineering. Biomaterials 2010, 31:9395-9405. 10.1016/j.biomaterials.2010.08.051, 3111806, 20932572.
11. Kyle S, McPherson MJ, Aggeli A, Ingham E. Rational molecular design of complementary self-assembling peptide hydrogels. Adv Healthcare Mater in press.
12. Riley JM, Aggeli A, Koopmans RJ, McPherson MJ. Bioproduction and characterization of a pH responsive self-assembling peptide. Biotechnol Bioeng 2009, 103:241-251. 10.1002/bit.22274, 19266471.
13. Kyle S, Aggeli A, Ingham E, McPherson MJ. Production of self-assembling biomaterials for tissue engineering. Trends Biotechnol 2009, 27:423-433. 10.1016/j.tibtech.2009.04.002, 2828541, 19497631.
14. McPherson MJ, James K, Kyle S, Parsons S, Riley J. Recombinant production of self-assembling peptides. Engineering aspects of self-organising materials. Volume 35 2009, 80-117. Koopmans RJ: Elsevier.
15. Hartmann BM, Kaar W, Falconer RJ, Zeng B, Middelberg APJ. Expression and purification of a nanostructure-forming peptide. J Biotechnol 2008, 135:85-91. 10.1016/j.jbiotec.2008.03.003, 18436322.
16. Studier FW. Protein production by auto-induction in high-density shaking cultures. Protein Expres Purif 2005, 41:207-234.
17. Hartmann BM, Kaar W, Yoo IK, Lua LH, Falconer RJ, Middelberg AJP. The chromatography-free release, isolation and purification of recombinant peptide for fibril self-assembly. Biotechnol Bioeng 2009, 104:973-985. 10.1002/bit.22447, 19530081.
18. Satakarni M, Curtis R. Production of recombinant peptides as fusions with SUMO. Protein Expres Purif 2011, 78:113-119.
19. Malakhov M, Mattern M, Malakhova O, Drinker M, Weeks S, Butt T. SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins. J Struct Funct Genomics 2004, 5:75-86.
20. Lee CD, Sun HC, Hu SM, Chiu CF, Homhuan A, Liang SM, Leng CH, Wang TF. An improved SUMO fusion protein system for effective production of native proteins. Protein Sci 2008, 17:1241-1248. 10.1110/ps.035188.108, 2442006, 18467498.
21. van Hell AJ, Costa C, Flesch FM, Sutter M, Jiskoot W, Crommelin DJA, Hennink WE, Mastrobattista E. Self-assembly of recombinant amphiphilic oligopeptides into vesicles. Biomacromolecules 2007, 8:2753-2761. 10.1021/bm0704267, 17696394.
22. Satakarni M, Koutinas AA, Webb C, Curtis R. Enrichment of fermentation media and optimization of expression conditions for the production of EAK16 peptide as fusions with SUMO. Biotechnol Bioeng 2009, 102:725-735. 10.1002/bit.22114, 18973282.
23. Bosse-Doenecke E, Weininger U, Gopalswamy M, Balbach J, Knudsen SM, Rudolph R. High yield production of recombinant native and modified peptides exemplified by ligands for G-protein coupled receptors. Protein Expres Purif 2008, 58:114-121.
24. Butt TR, Edavettal SC, Hall JP, Mattern MR. SUMO fusion technology for difficult-to-express proteins. Protein Expres Purif 2005, 43:1-9.
25. Marblestone JG, Edavettal SC, Lim Y, Lim P, Zuo X, Butt TR. Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO. Protein Sci 2006, 15:182-189. 10.1110/ps.051812706, 2242369, 16322573.
26. Zuo X, Li S, Hall J, Mattern M, Tran H, Shoo J, Tan R, Weiss SR, Butt TR. Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli. J Struct Funct Genomics 2005, 6:103-111. 10.1007/s10969-005-2664-4, 16211506.
27. Guzzo CM, Yang DCH. Systematic analysis of fusion and affinity tags using human aspartyl-tRNA synthetase expressed in E. coli. Protein Expres Purif 2007, 54:166-175.
28. Li J, Zhang J, Zhang Z, Ma H, Zhang J, Zhang S. Production of bioactive human beta-defensin-4 in Escherichia coli using SUMO fusion partner. Protein J 2010, 29:314-319. 10.1007/s10930-010-9254-4, 20526896.
29. Sambrook J. Fritsch EF, Maniatis T: Molecular cloning: A laboratory manual 1989, New York: Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
30. Assenberg R, Delmas O, Graham SC, Verma A, Berrow N, Stuart DI, Owens RJ, Bourhy H, Grimes JM. Expression, purification and crystallization of a lyssavirus matrix (M) protein. Acta Cryst 2008, F64:258-262.