Combination of PB2 271A and SR polymorphism at positions 590/591 is critical for viral replication and virulence of swine influenza virus in cultured cells and in vivo

Journal of Virology

Volumn 86, Issue 2, 2012, Pages 1233-1237

  Liu, Qinfang ^a   Qiao, Chuanling ^a,c   Marjuki, Henju ^b   Bawa, Bhupinder ^a   Ma, Jingqun ^a   Guillossou, Stephane ^a   Webby, Richard J ^b   Richt, Jürgen A ^a   Ma, Wenjun ^a  

^a KANSAS STATE UNIVERSITY   (United States)

^b ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

^c Harbin Veterinary Research Institute   (China)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN PB1; PROTEIN PB2; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA;

2009 H1N1 INFLUENZA; ADAPTATION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; GENETIC POLYMORPHISM; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; INFLUENZA; INFLUENZA VIRUS A H1N1; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA TRANSCRIPTION; VIRUS MUTATION; VIRUS REPLICATION; VIRUS VIRULENCE;

ANIMALS; CELL LINE; HUMANS; INFLUENZA A VIRUS, H1N1 SUBTYPE; INFLUENZA, HUMAN; MICE; MUTATION; ORTHOMYXOVIRIDAE INFECTIONS; POLYMORPHISM, SINGLE NUCLEOTIDE; REASSORTANT VIRUSES; RNA REPLICASE; SWINE; SWINE DISEASES; VIRAL PROTEINS; VIRULENCE; VIRUS REPLICATION;

AVES; MAMMALIA; MUS; SIMIAN IMMUNODEFICIENCY VIRUS; SWINE INFLUENZA VIRUS;

EID: 84863128008     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.05699-11     Document Type: Article

References (21)

1. Berhane Y, et al. 2010. Molecular characterization of pandemic H1N1 influenza viruses isolated from turkeys and pathogenicity of a human pH1N1 isolate in turkeys. Avian Dis. 54:1275-1285.
2. Bussey KA, Bousse TL, Desmet EA, Kim B, Takimoto T. 2010. PB2 residue 271 plays a key role in enhanced polymerase activity of influenza A viruses in mammalian host cells. J. Virol. 84:4395-4406.
3. Dundon WG, De Benedictis P, Viale E, Capua I. 2010. Serologic evidence of pandemic (H1N1) 2009 infection in dogs, Italy. Emerg. Infect. Dis. 16:2019-2021.
4. Gabriel G, et al. 2005. The viral polymerase mediates adaptation of an avian influenza virus to a mammalian host. Proc. Natl. Acad. Sci. U. S. A. 102:18590-18595.
5. Herfst S, et al. 2010. Introduction of virulence markers in PB2 of pandemic swine-origin influenza virus does not result in enhanced virulence or transmission. J. Virol. 84:3752-3758.
6. Jagger BW, et al. 2010. The PB2-E627K mutation attenuates viruses containing the 2009 H1N1 influenza pandemic polymerase. mBio 1:e00067-10.
7. Li Z, et al. 2005. Molecular basis of replication of duck H5N1 influenza viruses in a mammalian mouse model. J. Virol. 79:12058-12064.
8. Ma W, et al. 2011. Pathogenicity of swine influenza viruses possessing an avian or swine-origin PB2 polymerase gene evaluated in mouse and pig models. Virology 410:1-6.
9. Mehle A, Doudna JA. 2009. Adaptive strategies of the influenza virus polymerase for replication in humans. Proc. Natl. Acad. Sci. U. S. A. 106: 21312-21316.
10. Munster VJ, et al. 2007. The molecular basis of the pathogenicity of the Dutch highly pathogenic human influenza A H7N7 viruses. J. Infect. Dis. 196:258-265.
11. Pasma T, Joseph T. 2010. Pandemic (H1N1) 2009 infection in swine herds, Manitoba, Canada. Emerg. Infect. Dis. 16:706-708.
12. Robb NC, Smith M, Vreede FT, Fodor E. 2009. NS2/NEP protein regulates transcription and replication of the influenza virus RNA genome. J. Gen. Virol. 90:1398-1407.
13. Shinya K, Watanabe S, Ito T, Kasai N, Kawaoka Y. 2007. Adaptation of an H7N7 equine influenza A virus in mice. J. Gen. Virol. 88:547-553.
14. Smith GJ, et al. 2009. Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic. Nature 459:1122-1125.
15. Sponseller BA, et al. 2010. Influenza A pandemic (H1N1) 2009 virus infection in domestic cat. Emerg. Infect. Dis. 16:534-537.
16. Steel J, Lowen AC, Mubareka S, Palese P. 2009. Transmission of influenza virus in a mammalian host is increased by PB2 amino acids 627K or 627E/701N. PLoS Pathog. 5:e1000252.
17. Subbarao EK, London W, Murphy BR. 1993. A single amino acid in the PB2 gene of influenza A virus is a determinant of host range. J. Virol. 67:1761-1764.
18. Webby RJ, et al. 2000. Evolution of swine H3N2 influenza viruses in the United States. J. Virol. 74:8243-8251.
19. Yamada S, et al. 2010. Biological and structural characterization of a host-adapting amino acid in influenza virus. PLoS Pathog. 6:e1001034.
20. Zhou NN, et al. 1999. Genetic reassortment of avian, swine, and human influenza A viruses in American pigs. J. Virol. 73:8851-8856.
21. Zhu H, et al. 2010. Substitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in mice. Virology 401:1-5.