Polycistronic coexpression and nondenaturing purification of histone octamers

Analytical Biochemistry

Volumn 427, Issue 2, 2012, Pages 190-192

  Shim, Yoonjung ^a   Duan, Ming Rui ^b   Chen, Xuejing ^a   Smerdon, Michael J ^b   Min, Jung Hyun ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

Chromatin; Histone; Nucleosome; Polycistronic vector

Indexed keywords

CHROMOSOMES;

BASIC BUILDING BLOCK; CHROMATIN; CO-EXPRESSION; GENETIC MECHANISM; HISTONE; HISTONE OCTAMERS; NUCLEOSOMES; SIMPLE METHOD;

PROTEINS;

HISTONE; HISTONE H2A; HISTONE H2B; HISTONE H3; HISTONE H4; RECOMBINANT PROTEIN;

ARTICLE; CHROMATIN; CHROMATOGRAPHY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GEL PERMEATION CHROMATOGRAPHY; NICKEL AFFINITY CHROMATOGRAPHY; NUCLEOSOME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

EID: 84863084595     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.05.006     Document Type: Article

References (15)

1. K.E. Van Holde, Chromatin, Springer Verlag, New York, 1989.
2. K. Luger, A.W. Mader, R.K. Richmond, D.F. Sargent, T.J. Richmond, Crystal structure of the nucleosome core particle at 2.8 Å resolution, Nature 389 (1997) 251-260. (Pubitemid 27406632)
3. E.I. Campos, D. Reinberg, Histones: annotating chromatin, Annu. Rev. Genet. 43 (2009) 559-599.
4. G.R. Schnitzler, Isolation of histones and nucleosome cores from mammalian cells, Curr. Protoc. Mol. Biol. (2001) unit 21.5.
5. D. Shechter, H.L. Dormann, C.D. Allis, S.B. Hake, Extraction, purification, and analysis of histones, Nat. Protoc. 2 (2007) 1445-1457.
6. K. Luger, T.J. Rechsteiner, T.J. Richmond, Expression and purification of recombinant histones and nucleosome reconstitution, Methods Mol. Biol. 119 (1999) 1-16.
7. P.N. Dyer, R.S. Edayathumangalam, C.L. White, Y. Bao, S. Chakravarthy, U.M. Muthurajan, K. Luger, Reconstitution of nucleosome core particles from recombinant histones and DNA, Methods Enzymol. 375 (2004) 23-44. (Pubitemid 38124858)
8. K. Luger, T.J. Rechsteiner, T.J. Richmond, Preparation of nucleosome core particle from recombinant histones, Methods Enzymol. 304 (1999) 3-19. (Pubitemid 29268874)
9. P.T. Lowary, J. Widom, New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning, J. Mol. Biol. 276 (1998) 19-42. (Pubitemid 28085408)
10. M.R. Duan, M.J. Smerdon, UV damage in DNA promotes nucleosome unwrapping, J. Biol. Chem. 285 (2010) 26295-26303.
11. R.D. Makde, J.R. England, H.P. Yennawar, S. Tan, Structure of RCC1 chromatin factor bound to the nucleosome core particle, Nature 467 (2010) 562-566.
12. G. Li, J. Widom, Nucleosomes facilitate their own invasion, Nat. Struct. Mol. Biol. 11 (2004) 763-769. (Pubitemid 39014502)
13. H. Weintraub, K. Palter, F. Van Lente, Histones H2a, H2b, H3, and H4 form a tetrameric complex in solutions of high salt, Cell 6 (1975) 85-110.
14. W. Selleck, S. Tan, Recombinant protein complex expression in E. coli, Curr. Protoc. Protein Sci. (2008) unit 5.21.
15. M. Anderson, J.H. Huh, T. Ngo, A. Lee, G. Hernandez, J. Pang, J. Perkins, R.N. Dutnall, Co-expression as a convenient method for the production and purification of core histones in bacteria, Protein Expr. Purif. 72 (2010) 194-204.