Development of a polymerase chain reaction (PCR) test for the detection of virulent forms of Vibrio parahaemolyticus

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 31, Issue 4, 2012, Pages 431-439

  Kadhim, H M ^a   Miah, A ^a   Munn, C B ^a   Gilpin, M L ^a  

^a UNIVERSITY OF PLYMOUTH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; LACTATE DEHYDROGENASE; OUTER MEMBRANE PROTEIN;

AMPLICON; ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CELL STRAIN CACO 2; CYTOTOXICITY; DNA SEQUENCE; ENZYME RELEASE; GENE AMPLIFICATION; GENOME SIZE; HOST PATHOGEN INTERACTION; HUMAN; HUMAN CELL; MOLECULAR CLONING; MOLECULAR TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RANDOM AMPLIFIED POLYMORPHIC DNA; TDH GENE; TRH GENE; TYPE III SECRETION SYSTEM; VIBRIO PARAHAEMOLYTICUS;

BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIOLOGICAL TECHNIQUES; CACO-2 CELLS; CELL SURVIVAL; CULTURE MEDIA; DNA PRIMERS; HUMANS; POLYMERASE CHAIN REACTION; VIBRIO PARAHAEMOLYTICUS; VIRULENCE; VIRULENCE FACTORS;

EID: 84862886034     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-011-1324-9     Document Type: Article

References (30)

1. Chao G, Jiao X, Zhou X, Yang Z, Huang J, Zhou L, Qian X (2009) Distribution, prevalence, molecular typing, and virulence of Vibrio parahaemolyticus isolated from different sources in coastal province Jiangsu, China. Food Control 20:907-912
2. Shirai H, Ito H, Hirayama T, Nakamoto Y, Nakabayashi N, Kumagai K, Takeda Y, Nishibuchi M (1990) Molecular epidemiologic evidence for association of thermostable direct hemolysin (TDH) and TDH-related hemolysin of Vibrio parahaemolyticus with gastroenteritis. Infect Immun 58:3568-3573 (Pubitemid 20353354)
3. Nishibuchi M, Kaper JB (1995) Thermostable direct hemolysin gene of Vibrio parahaemolyticus: a virulence gene acquired by a marine bacterium. Infect Immun 63:2093-2099
4. Cabrera-Garcia ME, Vazquez-Salinas C, Quinones-Ramirez EI (2004) Serologic and molecular characterization of Vibrio para-haemolyticus strains isolated from seawater and fish products of the Gulf of Mexico. Appl Environ Microbiol 70:6401-6406 (Pubitemid 39518543)
5. Cook DW, Bowers JC, DePaola A (2002) Density of total and pathogenic (tdh+) Vibrio parahaemolyticus in Atlantic and Gulf Coast molluscan shellfish at harvest. J Food Protection 65:1873-1880 (Pubitemid 36017017)
6. Islam MS, Tasmin R, Khan SI, Bakht HBM, Mahmood ZH, Rahman MZ, Bhuiyan NA, Nishibuchi M, Nair GB, Sack RB, Huq A, Colwell RR, Sack DA (2004) Pandemic strains of O3:K6 Vibrio parahaemolyticus in the aquatic environment of Bangladesh. Can J Microbiol 50:827-834 (Pubitemid 40293245)
7. Caburlotto G, Gennari M, Ghidini V, Tafi M, Lleo MM (2009) Presence of T3SS2 and other virulence-related genes in tdh-negative Vibrio parahaemolyticus environmental strains isolated from marine samples in the area of the Venetian Lagoon, Italy. FEMS Microbiol Ecol 70:506-514
8. Hervio-Heath D, Colwell RR, Derrien A, Robert-Pillot A, Fournier JM, Pommepuy M (2002) Occurrence of pathogenic vibrios in coastal areas of France. J Appl Microbiol 92:1123-1135 (Pubitemid 34535493)
9. Miyamoto Y, Kato T, Obara Y, Akiyama S, Takizawa K, Yamai S (1969) In vitro hemolytic characteristic of Vibrio parahaemolyticus: its close correlation with human pathogenicity. J Bacteriol 100:1147-1149
10. Honda T, Iida T (1993) The pathogenicity of Vibrio para-haemolyticus and the role of the thermostable direct heamolysin and related heamolysins. Rev Med Microbiol 4:106-113 (Pubitemid 23131684)
11. Park KS, Ono T, Rokuda K, Jang MH, Okada K, Iida T, Honda T (2004) Functional characterization of two type III secretion systems of Vibrio parahaemolyticus. Infect Immun 72:6659-6665 (Pubitemid 39425449)
12. Kodama T, Rokuda M, Park K-S, Cantarelli VV, Matsuda S, Iida T, Honda T (2007) Identification and characterization of VopT, a novel ADP- ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2. Cell Microbiol 9:2598-2609 (Pubitemid 47500513)
13. Kim YB, Okuda J, Matsumoto C, Takahashi N, Hashimoto S, Nishibuchi M (1999) Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J Clin Microbiol 37:1173-1177 (Pubitemid 29140634)
14. Lee CY, Pan SF, Chen CH (1995) Sequence of a cloned pR72H fragment and its use for detection of Vibrio parahaemolyticus in shellfish with the PCR. Appl Environ Microbiol 61:1311-1317
15. Nasu H, Iida T, Sugahara T, Yamaichi Y, Park KS, Yokoyama K, Makino K, Shinagawa H, Honda T (2000) A filamentous phage associated with recent pandemic Vibrio parahaemolyticus O3:K6 strains. J Clin Microbiol 38:2156-2161 (Pubitemid 30414331)
16. Okura M, Osawa R, Iguchi A, Arakawa E, Terajima J, Watanabe H (2003) Genotypic analyses of Vibrio parahaemolyticus and development of a pandemic group-specific multiplex PCR assay. J Clin Microbiol 41:4676-4682 (Pubitemid 37259776)
17. Okura M, Osawa R, Iguchi A, Takagi M, Arakawa E, Terajima J, Watanabe H (2004) PCR-based identification of pandemic group Vibrio parahaemolyticus with a novel group-specific primer pair. Micobiol Immunol 48:787-790 (Pubitemid 39421423)
18. Khan AA, McCarthy S, Wang R, Cerniglia CA (2002) Characterization of United States outbreak isolates of Vibrio parahaemolyticus using enterobacterial repetitive intergenic consensus (ERIC) PCR and development of a rapid PCR method for detection of O3: K6 isolates. FEMS Microbiol Lett 206:209-214 (Pubitemid 34117621)
19. Miah A (2009) Characterisation and molecular typing of clinical and nvironmental isolates of Vibrio parahaemolyticus. PhD Thesis, University of Plymouth
20. Tada J, Ohashi T, Nishimura N, Shirasaki Y, Ozaki H, Fukushima S, Takano J, Nishibuchi M, Takeda Y (1992) Detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by polymerase chain reaction. Mol Cell Probes 6:477-487
21. Makino K, Oshima K, Kurokawa K, Yokoyama K, Uda T, Tagomori K, Iijima Y, Najima M, Nakano M, Yamashita A, Kubota Y, Kimura S, Yasunaga T, Honda T, Shinagawa H, Hattori M, Iida T (2003) Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae. Lancet 361:743-749 (Pubitemid 36293629)
22. Izutsu K, Kurokawa K, Tashiro K, Kuhara S, Hayashi T, Honda T, Iida T (2008) Comparative genomic analysis using microarray demonstrates a strong correlation between the presence of the 80-kilobase pathogenicity island and pathogenicity in Kanagawa phenomenon-positive Vibrio parahaemolyticus strains. Infect Immun 76:1016-1023 (Pubitemid 351428061)
23. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403-410
24. Martinez-Urtaza J, Lozano-Leon A, DePaola A, Ishibashi M, Shimada K, Nishibuchi M, Liebana E (2004) Characterization of pathogenic Vibrio parahaemolyticus isolates from clinical sources in Spain and comparison with Asian and North American pandemic isolates. J Clin Microbiol 42:4672-4678 (Pubitemid 39362388)
25. Baffone W, Vittoria E, Campana R, Citterio B, Casaroli A, Pierfelici L (2005) Occurrence and expression of virulence-related properties by environmental halophilic Vibrio spp. in in vitro and in vivo systems. Food Control 16:451-457 (Pubitemid 40012374)
26. Ono T, Park KS, Ueta M, Iida T, Honda T (2006) Identification of proteins secreted via Vibrio parahaemolyticus type III secretion system 1. Infect Immun 74:1032-1042 (Pubitemid 43185540)
27. Nishibuchi M, Kaper JB (1990) Duplication and variation of the thermostable direct haemolysin (tdh) gene in Vibrio parahaemolyticus. Mol Microbiol 4:87-99
28. Nakayashiki T, Nishimura K, Inokuchi H (1995) Cloning and sequencing of a previously unidentified gene that is involved in the biosynthesis of heme in Escherichia coli. Gene 153:67-70
29. Yeung PS, Wiedmann M, Boor KJ (2007) Evaluation of a tissue culture-based approach for differentiating between virulent and avirulent Vibrio parahaemolyticus strains based on cytotoxicity. J Food Prot 70:348-354
30. Raimondi F, Kao JPY, Fiorentini C, Fabbri A, Donelli G, Gasparini N, Rubino A, Fasano A (2000) Enterotoxicity and cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin in vitro systems. Infect Immun 68:3180-3185 (Pubitemid 30340407)