Isolation and functional characterisation of the genes encoding Δ8-sphingolipid desaturase from brassica rapa

Journal of Genetics and Genomics

Volumn 39, Issue 1, 2012, Pages 47-59

  Li, Shu Fen ^a,b   Song, Li Ying ^a   Yin, Wei Bo ^a   Chen, Yu Hong ^a   Chen, Liang ^a,c   Li, Ji Lin ^c   Wang, Richard R C ^d   Hu, Zan Min ^a  

^a INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c HARBIN NORMAL UNIVERSITY   (China)

^d UTAH STATE UNIVERSITY   (United States)

Author keywords

8 Sphingolipid desaturase; Biochemical functional diversity; Brassica rapa; Expression pattern; Long chain base (LCB)

Indexed keywords

ALUMINUM; DELTA8 SPHINGOLIPID DESATURASE; SPHINGOLIPID; UNCLASSIFIED DRUG;

ARABIDOPSIS; ARTICLE; BRASSICA RAPA; BRD8A GENE; BRD8C GENE; BRD8D GENE; CATALYSIS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; GENE; GENE DOSAGE; GENE FUNCTION; GENE ISOLATION; HETEROLOGOUS EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; SACCHAROMYCES CEREVISIAE; TRANSGENIC ORGANISM; TRANSGENIC PLANT;

ALUMINUM; AMINO ACID SEQUENCE; ARABIDOPSIS; BIOCATALYSIS; BRASSICA RAPA; EVOLUTION, MOLECULAR; GENE DOSAGE; GENE EXPRESSION REGULATION, PLANT; INTRACELLULAR SPACE; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS, GENETIC; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, NUCLEIC ACID; SPHINGOSINE; TOBACCO;

ARABIDOPSIS; BRASSICA; BRASSICA RAPA; EMBRYOPHYTA; SACCHAROMYCES CEREVISIAE;

EID: 84862831913     PISSN: 16738527     EISSN: 18735533     Source Type: Journal    
DOI: 10.1016/j.jgg.2011.12.002     Document Type: Article

References (50)

1. Adams K.L., Wendel J.F. Novel patterns of gene expression in polyploidy plants. Trends Genet. 2005, 21:539-543.
2. Babula D., Kaczmarek M., Barakat A., Delseny M., Quiros C.F., Sadowski J. Chromosomal mapping of Brassica oleracea based on ESTs from Arabidopsis thaliana: complexity of the comparative map. Mol. Genet. Genomics 2003, 268:656-665.
3. Borner G.H.H., Sherrier D.J., Weimar T., Michaelson L.V., Hawkins N.D., MacAskill A., Napier J.A., Beale M.H., Lilley K.S., Dupree P. Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts. Plant Physiol. 2005, 137:104-116.
4. Chao D.Y., Gable K., Chen M., Baxter I., Dietrich C.R., Cahoon E.B., Guerinot M.L., Lahner B., Lü S., Markham J.E., Morrissey J., Han G., Gupta S.D., Harmon J.M., Jaworski J.G., Dunn T.M., Salt D.E. Sphingolipids in the root play an important role in regulating the leaf ionome in Arabidopsis thaliana. Plant Cell 2011, 23:1061-1081.
5. Chen M., Markham J.E., Dietrich C.R., Jaworski J.G., Cahoon E.B. Sphingolipid long-chain base hydroxylation is important for growth and regulation of sphingolipid content and composition in Arabidopsis. Plant Cell 2008, 20:1862-1878.
6. Chen X., Truksa M., Snyder C.L., El-Mezawy A., Shah S., Weselake R.J. Three homologous genes encoding sn-glycerol-3-phosphate acyltransferase 4 exhibit different expression patterns and functional divergence in Brassica napus. Plant Physiol. 2011, 155:851-865.
7. Clough S.J., Bent A.F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1998, 16:735-743.
8. Coursol S., Fan L.M., Stunff H.L., Spiegel S., Gilroy S., Assmann S.M. Sphingolipid signalling in Arabidopsis guard cells involves heterotrimeric G proteins. Nature 2003, 423:651-654.
9. da Silva A.L.S., Sperling P., Horst W., Franke S., Ott C., Becker D., Staβ A., Lörz H., Heinz E. A possible role of sphingolipids in the aluminium resistance of yeast and maize. J. Plant Physiol. 2006, 163:26-38.
10. Doyle J.J., Doyle J.L. A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 1987, 19:11-15.
11. Elble R. A simple and efficient procedure for transformation of yeasts. Biotechniques 1992, 13:18-20.
12. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985, 39:783-791.
13. 6-acyl-desaturases. Plant Mol. Biol. 2007, 64:241-250.
14. Hong C.P., Kwon S.J., Kim J.S., Yang T.J., Park B.S., Lim Y.P. Progress in understanding and sequencing the genome of Brassica rapa. Int. J. Plant Genomics 2008, 2008:582837-582846.
15. Jefferson R.A., Kavanagh T.A., Bevan M.W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987, 6:3901-3907.
16. Johnston J.S., Pepper A.E., Hall A.E., Chen Z.J., Hodnett G., Drabek J., Lopez R., Price H.J. Evolution of genome size in Brassicaceae. Ann. Bot 2005, 95:229-235.
17. Kawaguchi M., Imai H., Naoe M., Yasui Y., Ohnishi M. Cerebrosides in grapevine leaves: distinct composition of sphingoid bases among the grapevine species having different tolerances to freezing temperature. Biosci. Biotechnol. Biochem. 2000, 64:1271-1273.
18. Lagercrantz U. Comparative mapping between Arabidopsis thaliana and Brassica nigra indicates that Brassica genomes have evolved through extensive genome replication accompanied by chromosome fusions and frequent rearrangements. Genetics 1998, 150:1217-1228.
19. Lan T.H., DelMonte T.A., Reischmann K.P., Hyman J., Kowalski S.P., McFerson J., Kresovich S., Paterson A.H. An EST-enriched comparative map of Brassica oleracea and Arabidopsis thaliana. Genome Res. 2000, 10:776-788.
20. Li Z.G., Yin W.B., Song L.Y., Chen Y.H., Guan R.Z., Wang J.Q., Wang R.R.C., Hu Z.M. Genes encoding the biotin carboxylase subunit of acetyl-CoA carboxylase from Brassica napus and parental species: cloning, expression patterns, and evolution. Genome 2011, 46:202-211.
21. Lukens L., Zou F., Lydiate D., Parkin I., Osborn T. Comparison of a Brassica oleracea genetic map with the genome of Arabidopsis thaliana. Genetics 2003, 164:359-372.
22. Lynch D.V., Dunn T.M. An introduction to plant sphingolipids and a review of recent advances in understanding their metabolism and function. New Phytol. 2004, 161:677-702.
23. Markham J.E., Li J., Cahoon E.B., Jaworski J.G. Separation and identification of major plant sphingolipid classes from leaves. J. Biol. Chem. 2006, 281:22684-22694.
24. 8 sphingolipid desaturase from Aquilegia vulgaris. Biochem. Soc. Trans. 2002, 30:1073-1075.
25. Michaelson L.V., Zäuner S., Markham J.E., Haslam R.P., Desikan R., Mugford S., Albrecht S., Warnecke D., Sperling P., Heinz E., Napier J.A. Functional characterization of a higher plant sphingolipid Δ4-desaturase: defining the role of sphingosine and sphingosine-1-phosphate in Arabidopsis. Plant Physiol. 2009, 149:487-498.
26. Moreno-Pérez A.J., Martínez-Force E., Garcés R., Salas J.J. Sphingolipid base modifying enzymes in sunflower (Helianthus annuus): cloning and characterization of a C4-hydroxylase gene and a new paralogous Δ8-desaturase gene. J. Plant Physiol. 2011, 168:831-839.
27. Murashige T., Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant 1962, 15:473-497.
28. Nelson B.K., Cai X., Nebenführ A. A multicolored set of invivo organelle markers for co-localization studies in Arabidopsis and other plants. Plant J. 2007, 51:1126-1136.
29. Nishikawa M., Hosokawa K., Ishiguro M., Minamioka H., Tamura K., Hara-Nishimura I., Takahashi Y., Shimazaki K., Imai H. Degradation of sphingoid long-chain base 1-phosphates (LCB-1Ps): functional characterization and expression of AtDPL1 encoding LCB-1P lyase involved in the dehydration stress response in Arabidopsis. Plant Cell Physiol. 2008, 49:1758-1763.
30. Parkin I.A.P., Sharpe A.G., Lydiate D.J. Patterns of genome duplication within the Brassica napus genome. Genome 2003, 46:291-303.
31. Park S., Yu H.J., Mun J.H., Lee S.C. Genome-wide discovery of DNA polymorphism in Brassica rapa. Mol. Genet. Genomics 2010, 283:135-145.
32. Pruett S.T., Bushnev A., Hagedorn K., Adiga M., Haynes C.A., Sullards M.C., Liotta D.C., Merrill A.H. Biodiversity of sphingoid bases (" sphingosines" ) and related amino alcohols. J. Lipid Res. 2008, 49:1621-1639.
33. Roudier F., Schindelmann G., DeSalle R., Benfey P.N. The COBRA family of putative GPI-anchored proteins in Arabidopsis. A new fellowship in expansion. Plant Physiol. 2002, 130:538-548.
34. Ryan P.R., Liu Q., Sperling P., Dong B., Franke S., Delhaize E. A higher plant Δ8 sphingolipid desaturase with a preference for (Z)-isomer formation confers aluminum tolerance to yeast and plants. Plant Physiol. 2007, 144:1968-1977.
35. Shanklin J., Whittle E., Fox B.G. Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry 1994, 33:12787-12794.
36. 6-desaturase from Ribes nigrum. J. Exp. Bot. 2010, 61:1827-1838.
37. 8-sphingolipid desaturase from higher plants. Biochem. Soc. Trans. 2000, 28:638-641.
38. Sperling P., Franke S., Lüthje S., Heinz E. Are glucocerebrosides the predominant sphingolipids in plant plasma membranes?. Plant Physiol. Biochem. 2005, 43:1031-1038.
39. Sperling P., Heinz E. Plant sphingolipids: structural diversity, biosynthesis, first genes and functions. Biochim. Biophys. Acta 2003, 1632:1-15.
40. 8-sphingolipid desaturase from Borago officinalis. Arch. Biochem. Biophys. 2001, 388:293-298.
41. Sperling P., Zähringer U., Heinz E. A sphingolipid desaturase from higher plants. Identification of a new cytochrome b5 fusion protein. J. Biol. Chem. 1998, 273:28590-28596.
42. Takahashi Y., Berberich T., Kanzaki H., Matsumura H., Saitoh H., Kusano T., Terauchi R. Serine palmitoyltransferase, the first step enzyme in sphingolipid biosynthesis, is involved in nonhost resistance. Mol. Plant Microbe Interact. 2009, 22:31-38.
43. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007, 24:1596-1599.
44. Teng C., Dong H., Shi L., Deng Y., Mu J., Zhang J., Yang X., Zuo J. Serine palmitoyltransferase, a key enzyme for de novo synthesis of sphingolipids, is essential for male gametophyte development in Arabidopsis. Plant Physiol. 2008, 146:1322-1332.
45. Town C.D., Cheung F., Maiti R., Crabtree J., Haas B.J., Wortman J.R., Hine E.E., Althoff R., Arbogast T.S., Tallon L.J., Vigouroux M., Trick M., Bancroft I. Comparative genomics of Brassica oleracea and Arabidopsis thaliana reveal gene loss, fragmentation, and dispersal after polyploidy. Plant Cell 2006, 18:1348-1359.
46. Umemura K., Ogawa N., Koga J., Iwata M., Usami H. Elicitor activity of cerebroside, a sphingolipid elicitor, in cell suspension cultures of rice. Plant Cell Physiol. 2002, 43:778-784.
47. Wang W., Yang X., Tangchaiburana S., Ndeh R., Markham J.E., Tsegaye Y., Dunn T.M., Wang G.L., Bellizzi M., Parsons J.F., Morrissey D., Bravo J.E., Lynch D.V., Xiao S. An inositolphosphorylceramide synthase is involved in regulation of plant programmed cell death associated with defense in Arabidopsis. Plant Cell 2008, 20:3163-3179.
48. Xiong T.C., Coursol S., Grat S., Ranjeva R., Mazars C. Sphingolipid metabolites selectively elicit increases in nuclear calcium concentration in cell suspension cultures and in isolated nuclei of tobacco. Cell Calcium 2008, 43:29-37.
49. Yang T.J., Lai K.N., Tai P.Y., Li W.H. Rates of nucleotide substitution in angiosperm mitochondrial DNA sequences and dates of divergence between Brassica and other angiosperm lineages. J. Mol. Evol. 1999, 48:597-604.
50. Zäuner S., Ternes P., Warnecke D. Biosynthesis of sphingolipids in plants (and some of their functions). Adv. Exp. Med. Biol. 2010, 688:249-263.