Expression and characterization of a recombinant Cry1Ac crystal protein fused with an insect-specific neurotoxin ω-ACTX-Hv1a in Bacillus thuringiensis

Gene

Volumn 498, Issue 2, 2012, Pages 323-327

  Li, W P ^a   Xia, L Q ^a   Ding, X Z ^a   Lv, Y ^a   Luo, Y S ^a   Hu, S B ^a   Yin, J ^a   Yan, F ^a  

^a HUNAN NORMAL UNIVERSITY   (China)

Author keywords

Crystallization; Fusion gene; Insecticidal activity; Neurotoxin

Indexed keywords

CRY1AC OMEGA ACTX HV1A FUSION PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; INSECTICIDE; NEUROTOXIN; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS THURINGIENSIS; GENE EXPRESSION; INSECT; INSECTICIDAL ACTIVITY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SPODOPTERA EXIGUA; WESTERN BLOTTING;

ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTOXINS; GREEN FLUORESCENT PROTEINS; HEMOLYSIN PROTEINS; INCLUSION BODIES; INSECTICIDES; LARVA; RECOMBINANT FUSION PROTEINS; SPIDER VENOMS; SPODOPTERA;

BACILLUS THURINGIENSIS; HEXAPODA; SPODOPTERA EXIGUA;

EID: 84862828768     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2012.01.034     Document Type: Article

References (31)

1. Arantes O., Lereclus D. Construction of cloning vectors for Bacillus thuringiensis. Gene 1991, 108(1):115-119.
2. Arvidson H., Dunn P.E., Stmad S., Aronson A.I. Specificity of Bacillus thuringiensis for lepidopteran larvae: factors involved in vivo and in the structure of a purified protoxin. Mol. Microbiol. 1989, 3(11):1533-1543.
3. Atkinson R.K., Howden M.E.H., Tyler M.I., Vonarx E.J. Insecticidal toxins derived from funnel web (Atrax or Hadronyche) spiders 1998, US Patent No. 5,763,568, Zeneca Limited, USA.
4. Bietlot H.P., Vishmulhatla I., Carey P.R., Pozsgay M., Kaplan H. Characterization of the cysteine residues and disulphide linkages in the protein crystal of Bacillus thuringiensis. Biochem. J. 1990, 267(2):309-315.
5. De Maagd R.A., Weemen-Hendriks M., Stiekema W., Bosch D. Bacillus thuringiensis delta- endotoxin Cry1C domainIII can function as a specificity determinant for Spodoptera exigua in different but not all Cry1-Cry1C hybrids. Appl. Environ. Microbiol. 2000, 66(4):1559-1563.
6. Dean D.H., et al. Probing the mechanism of action of Bacillus thuringiensis insecticidal proteins by site-directed mutagenesis - a minireview. Gene 1996, 179(1):111-117.
7. Ding X.Z., Xia L.Q. Selection of a High Toxic Insecticide Strain 4.0718 of Bacillus thuringiensis. Chin. J. Biol. Control 2001, 17(4):163-166.
8. Ding X.Z., Luo Z.H., Xia L.Q., Gao B.D., Sun Y.J., Zhang Y.M. Improving the insecticidal activity by expression of a recombinant cry1Ac gene with chitinase-encoding gene in acrystalliferous Bacillus thuringiensis. Curr. Microbiol. 2008, 56(5):442-446.
9. Finney D.J. Probit Analysis 1971, Cambridge University Press, Cambridge, UK.
10. Fletcher J.I., et al. The structure of a novel insecticidal neurotoxin, ω-atracotoxin-HV1, from the venom of an Australian funnel web spider. Nat. Struct. Biol. 1997, 4(7):559-566.
11. Georghiou G.P., Wirth M.C. Influence of exposure to single versus multiple toxins of Bacillus thuringiensis subsp. israelensis on development of resistance in the mosquito Culex quinquefasciatus (Diptera: Culicidae). Appl. Environ. Microbiol. 1997, 63(3):1095-1101.
12. Hernández C.S., Ferré J. Common receptor for Bacillus thuringiensis toxins Cry1Ac, Cry1Fa, and Cry1Ja in Helicoverpa armigera, Helicoverpa zea, and Spodoptera exigua. Appl. Environ. Microbiol. 2005, 71(9):5627-5629.
13. Hernández-Martínez P., Ferré J., Escriche B. Susceptibility of Spodoptera exigua to 9 toxins from Bacillus thuringiensis. J. Invertebr. Pathol. 2007, 97:245-250.
14. Hu S.B., et al. Efficient constitutive expression of chitinase in the mother cell of Bacillus thuringiensis and its potential to enhance the toxicity of Cry1Ac protoxin. Appl. Microbiol. Biotechnol. 2009, 82(6):1157-1167.
15. Jiang H., Zhu Y.X., Chen Z.L. Insect resistance of transformed tobacco plants with gene of the spider insecticidal peptide. Acta Bot. Sin. 1996, 38(2):95-99.
16. Lopez L.P., Garay C.M., Porta H., Almazan C.R., Soberon M., Bravo A. Strategies to improve the insecticidal activity of Cry toxins from Bacillus thuringiensis. Peptides 2009, 30(3):589-595.
17. O'Rourke M.E., Sappington T.W., Fleischer S.J. Managing resistance to B. t. crops in a genetically variable insect herbivore, Ostrinia nubilalis. Ecol. Appl. 2009, 20(5):1228-1236.
18. Park H.W., Bideshi D.K., Federici B.A. Molecular genetic manipulation of truncated Cry1C protein synthesis in Bacillus thuringiensis to improve stability and yield. Appl. Environ. Microbiol. 2000, 66(10):4449-4455.
19. Raymond B., Wright D.J., Bonsall M.B. Effects of host plant and genetic background on the fitness costs of resistance to Bacillus thuringiensis 2010, (Jun 2, Epub ahead of print).
20. Sedlak M., Walter T., Aronson A. Regulation by overlapping promoters of the rate of synthesis and deposition into crystalline inclusions of Bacillus thuringiensis δ-Endotoxins. J. Bacteriol. 2000, 182(3):734-741.
21. Shao Z.Z., Liu Z.D., Yu Z.N. Effects of the 20-kiloDalton helper protein on Cry1Ac production and spore formation in Bacillus thuringiensis. Appl. Environ. Microbiol. 2001, 67(12):5362-5369.
22. Singh A.K., Rembold H. Maintenance of the cotton bollworm Heliothis armigera in the laboratory culture. I. Rearing on semisynthetic diet. Insect Sci. Appl. 1992, 13:333-338.
23. Stahly D.P., Dingman D.W., Bulla L.A., Aronson A.I. Possible origin and function of the parasporal crystals in Bacillus thuringiensis. Biochem. Biophys. Res. Commun. 1978, 84(3):581-588.
24. Sun Y.J., Fu Z.J., Ding X.Z., Xia L.Q. Evaluating the insecticidal genes and their expressed products in Bacillus thuringiensis strains by combining PCR with mass spectrometry. Appl. Environ. Microbiol. 2008, 74(21):6811-6813.
25. Tabashnik B.E. Managing resistance with multiple pesticide tactics: theory, evidence and recommendations. J. Econ. Entomol. 1989, 82(5):1263-1269.
26. Tedford H.W., Fletcher J.I., King G.F. Functional significance of the β-hairpin in the insecticidal neurotoxin ω-atracotoxin-Hv1a. J. Biol. Chem. 2001, 276(28):26568-26576.
27. Tedford H.W., Gilles N., Menez A., Doering C.J., Zamponi G.W., King G.F. Scanning mutagenesis of omega-atracotoxin-Hv1a reveals a spatially restricted epitope that confers selective activity against insect calcium channels. J. Biol. Chem. 2004, 279(42):44133-44140.
28. Tedford H.W., Sollod B.L., Maggio F., King G.F. Australian funnel-web spiders: master insecticide chemists. Toxicon 2004, 43(5):601-618.
29. Van Frankenhuyzen K. Insecticidal activityof Bacillus thuringiensis crystal proteins. J. Invertebr. Pathol. 2009, 101(1):1-16.
30. Xia L.Q., Wang F.X., Ding X.Z., Zhao X.M., Fu Z.J., Quan M.F., Yu Z.N. The role of β18-β19 loop structure in insecticidal activity of Cry1Ac toxin from Bacillus thuringiensis. Chin. Sci. Bull. 2008, 53:1-7.
31. Zheng S.S., An C.C., Li Q.R., Chen Z.L. Study of transferring fusion protein gene of the spider insecticidal peptide and Bt-toxin C-peptide into cotton. Cotton Sci. 2002, 14(6):348-351.