Efficient constitutive expression of chitinase in the mother cell of Bacillus thuringiensis and its potential to enhance the toxicity of Cry1Ac protoxin

Applied Microbiology and Biotechnology

Volumn 82, Issue 6, 2009, Pages 1157-1167

  Hu, S B ^a   Liu, P ^a   Ding, X Z ^a   Yan, L ^a   Sun, Y J ^a   Zhang, Y M ^a   Li, W P ^a   Xia, L Q ^a  

^a HUNAN NORMAL UNIVERSITY   (China)

Author keywords

Biological control of pests; Chitinolytic activity; Inclusion body; Insecticidal activity; Insecticidal crystal proteins; Solubility property

Indexed keywords

BIOLOGICAL CONTROL OF PESTS; CHITINOLYTIC ACTIVITY; INCLUSION BODY; INSECTICIDAL ACTIVITY; INSECTICIDAL CRYSTAL PROTEINS; SOLUBILITY PROPERTY;

AMINES; BACTERIOLOGY; CLONING; GENE ENCODING; PH EFFECTS; PROTEINS; SODIUM; SOLUBILITY; TERNARY SYSTEMS;

INCLUSIONS;

CHITINASE; CRY1AC TOXIN;

BACTERIUM; BIOCONTROL AGENT; BIOLOGICAL CONTROL; BIOPESTICIDE; BIOTECHNOLOGY; CHITIN; ENZYME ACTIVITY; GENE EXPRESSION; LARVA; MOTH; SOLUBILITY; TOXIN;

ARMYWORM; ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL GENE; ENZYME ACTIVITY; ENZYME ANALYSIS; GENE SEQUENCE; INSECTICIDAL ACTIVITY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RECOMBINANT PLASMID; SHUTTLE VECTOR;

ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; CHITINASE; CLONING, MOLECULAR; DNA, BACTERIAL; ENDOTOXINS; GENE EXPRESSION; GENETIC VECTORS; HEMOLYSIN PROTEINS; LEPIDOPTERA; PROMOTER REGIONS, GENETIC; SPODOPTERA;

BACILLUS THURINGIENSIS; HELICOVERPA ARMIGERA; SPODOPTERA EXIGUA;

EID: 63949088397     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-1910-2     Document Type: Article

References (50)

1. H Agaisse D Lereclus 1995 How does Bacillus thuringiensis produce so much insecticidal crystal protein? J Bacteriol 177 6027 6032
2. O Arantes D Lereclus 1991 Construction of cloning vectors for Bacillus thuringiensis Gene 108 115 119
3. A Aronson 1995 The protoxin composition of Bacillus thuringiensis insecticidal inclusions affects solubility and toxicity Appl Environ Microbiol 61 4054 4060
4. AI Aronson ES Han W McGaughey D Johnson 1991 The solubility of inclusion proteins from Bacillus thuringiensis is dependent upon protoxin composition and is a factor in toxicity to insects Appl Environ Microbiol 57 981 986
5. N Arora T Ahmad R Rojagopal RK Bhatnagar 2003 A constitutively expressed 36 kDa exochitinase from Bacillus thuringiensis HD-1 Biochem Biophys Res Commun 307 620 625
6. N Arora A Selvapandiyan N Agrawal RK Bhatnagar 2003 Relocating expression of vegetative insecticidal protein into mother cell of Bacillus thuringiensis Biochem Biophys Res Commun 310 158 162
7. H Arvidson PE Dunn S Stmad AI Aronson 1989 Specificity of Bacillus thuringiensis for lepidopteran larvae: factors involved in vivo and in the structure of a purified protoxin Mol Microbiol 3 1533 1543
8. JE Barboza-Corona E Nieto-Mazzocco R Velazquez-Robledo R Salcedo-Harnandez M Bautista B Jiménez JE Ibarra 2003 Cloning, sequencing, and expression of the chitinase gene chiA74 from Bacillus thuringiensis Appl Environ Microbiol 69 1023 1029
9. JA Baum T Malvar 1995 Regulation of insecticidal crystal protein production in Bacillus thuringiensis Mol Microbiol 18 1 12
10. M Bradford 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254
11. YJ Cai JP Yan XM Hu B Han ZM Yuan 2007 Improving the insecticidal activity against resistant Culex quinquefasciatus mosquitoes by expression of chitinase gene chiAC in Bacillus sphaericus Appl Environ Microbiol 73 7744 7746
12. G Casique-Arroyo D Bideshi R Salcedo-Hernandez JE Barboza-Corona 2006 Development of a recombinant strain of Bacillus thuringiensis subsp. kurstaki HD-73 that produces the endochitinase ChiA74 Antonie van Leeuwenhoek 92 1 9
13. J Chen L Tang M Tang Y Shi Y Pang 2002 Cloning and expression product of vip3A gene from Bacillus thuringiensis and analysis of insecticidal activity Chin J Biotechnol 18 687 692
14. J Chen F Sun Y Shi W Xu W Guo Y Pang 2005 Efficient expression of vip184ΔP gene under the control of promoters plus Shine-Dalgarno (SD) sequences of cry genes from Bacillus thuringiensis J Appl Microbiol 99 426 434
15. XZ Ding LQ Xia 2001 Selection of a high toxic insecticide strain 4.0718 of Bacillus thuringiensis Chin J Biol Control 17 163 166
16. XZ Ding ZH Luo LQ Xia BD Gao YJ Sun YM Zhang 2008 Improving the insecticidal activity by expression of a recombinant cry1Ac gene with chitinase-encoding gene in acrystalliferous Bacillus thuringiensis Curr Microbiol 56 442 446
17. F Driss M Kallassy-Awad N Zouari S Jaoua 2005 Molecular characterization of a novel chitinase from Bacillus thuringiensis subsp. kurstaki J Appl Microbiol 99 945 953
18. Finney DJ (1971) Probit analysis. Cambridge University Press, Cambridge, UK
19. MF Glatron G Rapoport 1972 Biosynthesis of the parasporal inclusion of Bacillus thuringiensis: half-life of its corresponding messenger RNA Biochimie 54 1291 1301
20. B Ge D Bideshi WJ Moar BA Federici 1998 Differential effects of helper proteins encoded by the cry2A and cry11A operons on the formation of Cry2A inclusions in Bacillus thuringiensis FEMS Microbiol Lett 165 35 41
21. G Honee B Visser 1993 The mode of action of Bacillus thuringiensis crystal proteins Entomol Exp App 169 145 155
22. HY Hu LQ Xia HJ Shi 2004 Cloning and superexpression of cry1Ac gene from 20kb DNA associated with Bacillus thuringiensis Cry1A crystal protein Chinese J Biotechnol 20 656 661
23. M Lertcanawanichakul C Wiwat A Bhumiratana DH Dean 2004 Expression of Chitinase-encoding genes in Bacillus thuringiensis and toxicity of engineered B. thuringiensis subsp. aizawai toward Lymantria dispar larvae Curr microbiol 48 175 181
24. M Liu QX Cai HZ Liu BH Zhang JP Yan ZM Yuan 2002 Chitinolytic activities in Bacillus thuringiensis and their synergistic effects on larvicidal activity J Appl Microbiol 93 374 379
25. JE López-Meza BA Federici WJ Poehner AM Martínez-Castillo JE Ibarra 1995 Highly mosquitocidal isolates of Bacillus thuringiensis subspecies kenyae and entomocidus from Mexico Biochem Syst Ecol 23 461 468
26. O Lysenko 1976 Chitinase of Serratia marcescens and its toxicity to insects J Invertebr Pathol 27 385 386
27. ON Morris 1976 A 2 year study of the efficacy of Bacillus thuringiensis-chitinase combinations in spruce budworm (Choristoneura fumiferana) control Can Entomol 108 3225 3233
28. Nation JL (2001) Insect physiology and biochemistry. CRC, Boca Raton, pp 40-41
29. S Okay BE Tefon M Ozkan G Ozcengiz 2007 Expression of chitinase A (chiA) gene from a local isolate of Serratia marcescens in Coleoptera-specific Bacillus thuringiensis J Appl Microbiol 104 161 170
30. H Park B Ge LS Bauer BA Fedrici 1998 Optimization of Cry3A yields in Bacillus thuringiensis by use of sporulation-dependent promoters in combination with the STAB-SD mRNA sequence Appl Environ Microbiol 64 3932 3938
31. HW Park DK Bideshi BA Federici 2000 Molecular genetic manipulation of truncated Cry1C protein synthesis in Bacillus thuringiensis to improve stability and yield Appl Environ Microbiol 66 4449 4455
32. S Poncet A Delecluse G Anello A Klier G Rapoport 1994 Transfer and expression of the cryIVB and cryIVD genes of Bacillus thuringiensis subsp. Israelensis in Bacillus sphaericus 2297 FEMS Microbiol Lett 117 91 96
33. A Regev M Keller N Strizhov B Sheh E Prudovsky I Chet I Ginzberg Z Koncz-Kalman 1996 Synergistic activity of a Bacillus thuringiensis δ-endotoxin and a bacterial endochitinase against Spodoptera littoralis larvae Appl Environ Microbiol 62 3581 3586
34. LI Rojas-Avelizapa R Cruz-Camarillo MI Guerrero R Rodriguez-Vazquez JE Ibarra 1999 Selection and characterization of a proteo-chitinolytic strain of Bacillus thuringiensis able to grow in waste media World J Microbiol Biotechnol 15 261 268
35. Sambrook J, Fritsh EF, Maniatis T (1989) Molecular cloning. A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
36. MN Sampson GW Gooday 1998 Involvement of chitinases of Bacillus thuringiensis during pathogenesis in insects Microbiology 144 189 2194
37. AK Singh H Rembold 1992 Maintenance of the cotton bollworm Heliothis armigera in the laboratory culture. I. Rearing on semi synthetic diet Insect Sci Appl 13 333 338
38. N Sirichotpakorn P Rongnoparut K Choosang W Panbangred 2001 Coexpression of chitinase and the cry11Aa1 toxin genes in Bacillus thuringiensis serovar israelensis J Invertebr Pathol 78 160 169
39. WA Smirnoff 1974 Three years of aerial field experiments with Bacillus thuringiensis plus chitinase formulation against the spruce bud worm J Invertebr Pathol 24 344 348
40. DP Stahly DW Dingman LA Bulla AI Aronson 1978 Possible origin and function of the parasporal crystals in Bacillus thuringiensis Biochem Biophys Res Commun 84 581 588
41. YP Tan WP Donovan 2000 Deletion of aprA and nprA genes for alkaline protease A and neutral protease A from Bacillus thuringiensis: effect on insecticidal crystal proteins J Biotechnol 84 67 72
42. S Tantimavanich S Pantowatana A Bhumiratana W Panbangred 1997 Cloning of a chitinase gene into Bacillus thuringiensis subsp. Aizawai for enhanced insecticidal activity J Gen Appl Microbiol 43 31 37
43. S Thamthiankul S Suan-Nday S Tantimavanich W Panbangred 2001 Chitinase from Bacillus thuringiensis subsp. Pakistani Appl Microbiol Biotechnol 56 395 401
44. S Thamthiankul WJ Moar ME Miller W Panbangred 2004 Improving the insecticidal activity of Bacillus thuringiensis subsp. aizawai against Spodoptera exigua by chromosomal expression of a chitinase gene Appl Microbiol Biotechnol 65 183 192
45. H Tsujibo Y Yoshida K Miyamoto 1992 Purification, properties, and partial amino acid sequence of chitinase from a marion Alteromonas sp. strain O-7 Can J Microbiol 38 891 897
46. H Tsujibo K Minoura K Miyamoto H Endo M Moriwaki Y Inamori 1993 Purification and properties of a thermostable chitinase from Streptomyces thermoviolaceus OPC-520 Appl Environ Microbiol 59 620 622
47. C Wiwat S Thaithanun S Pantuwatana A Bhumiratana 2000 Toxicity of chitinase-producing Bacillus thuringiensis sp. Kurstaki HD-1 toward Plutella xylostella J Invertebr Pathol 76 270 277
48. HC Wong HE Schnepf HR Whiteley 1983 Transcription and translation start site for the Bacillus thuringiensis crystal protein gene J Biol Chem 258 1960 1967
49. D Wu BA Federici 1993 A 20-kilodalton protein preserves cell viability and promotes CytA crystal formation during sporulation in Bacillus thuringiensis J Bacteriol 175 5276 5280
50. LQ Xia YJ Sun XZ Ding ZJ Fu XT Mo H Zhang ZM Yuan 2005 Identification of cry-type genes on 20-kb DNA associated with Cry1 crystal proteins from Bacillus thuringiensis Cur Microbiol 51 53 58