Metabolic flux analysis of the central carbon metabolism of the industrial vitamin B 12 producing strain Pseudomonas denitrificans using 13C-labeled glucose

Journal of the Taiwan Institute of Chemical Engineers

Volumn 43, Issue 2, 2012, Pages 181-187

  Wang, Ze Jian ^a   Wang, Ping ^b   Liu, Yu Wei ^a   Zhang, Yi Ming ^c   Chu, Ju ^a   Huang, Ming zhi ^a   Zhuang, Ying Ping ^a   Zhang, Si Liang ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b HEBEI MEDICAL UNIVERSITY   (China)

^c CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

Author keywords

13C metabolic network analysis; Oxygen limiting level; Pseudomonas denitrificans; Vitamin B 12

Indexed keywords

CARBON METABOLISM; COFACTORS; ENTNER-DOUDOROFF; GLUCOSE CONSUMPTION; LOW OXYGEN; METABOLIC FLUX; METABOLIC FLUX ANALYSIS; METABOLIC NETWORK ANALYSIS; METHYL GROUP; NETWORK TOPOLOGY; PENTOSE PHOSPHATE PATHWAY; PROTEINOGENIC AMINO ACIDS; PSEUDOMONAS DENITRIFICANS; SERINE HYDROXYMETHYL TRANSFERASE; SPECIFIC OXYGEN UPTAKE RATE; THREONINE ALDOLASE ACTIVITY; VITAMIN B; VITAMIN B 12;

AMINO ACIDS; BACTERIA; CARBON; CULTIVATION; ELECTRIC NETWORK TOPOLOGY; FUNCTIONAL GROUPS; GLUCOSE; METHANOL; OXYGEN; PHYSIOLOGY;

METABOLISM;

EID: 84862823912     PISSN: 18761070     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jtice.2011.09.002     Document Type: Article

References (20)

1. 12. Appl Microbiol Biotechnol 2002, 58:275-285.
2. 12 by Pseudomonas denitrificans. Bioprocess Biosystems Eng 2008, 31:605-610.
3. 12 production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process. Bioresour Technol 2010, 101:2845-2852.
4. 12: a 35-year odyssey. J Org Chem 2003, 68:2529-2539.
5. 12). Nat Prod Rep 2002, 19:390-412.
6. Borodina I., Scholler C., Eliasson A., Nielsen J. Metabolic network analysis of Streptomyces tenebrarius, a Streptomyces species with an active Entner-Doudoroff pathway. Appl Environ Microbiol 2005, 71:2294-2302.
7. Christensen B., Nielsen J. Isotopomer analysis using GC-MS. Metab Eng 1999, 1:282-290.
8. 13C-based metabolic flux analysis. Nat Protoc 2009, 4:878-892.
9. Wierckx N., Ruijssenaars H.J., de Winde J.H., Schmid A., Blank L.M. Metabolic flux analysis of a phenol producing mutant of Pseudomonas putida S12: verification and complementation of hypotheses derived from transcriptomics. J Biotechnol 2009, 143:124-129.
10. 13C-experiments and gas chromatography-mass spectrometry data: protocol and principles. Methods Mol Biol 2007, 358:177-197.
11. 13C labeled isotopes. Metab Eng 2007, 9:387-405.
12. Blank L.M., Ionidis G., Ebert B.E., Buhler B., Schmid A. Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase. FEBS J 2008, 275:5173-5190.
13. Fischer E., Sauer U. Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS. Eur J Biochem 2003, 270:880-891.
14. Eisenberg R.C., Butters S.J., Quay S.C., Friedman S.B. Glucose uptake and phosphorylation in Pseudomonas fluorescens. J Bacteriol 1974, 120:147-153.
15. Nam H., Lee J., Lee D. Computational identification of altered metabolism using gene expression and metabolic pathways. Biotechnol Bioeng 2009, 103:835-843.
16. 13C metabolic flux analysis. Metab Eng 2001, 3:265-283.
17. 13C-labeled glucose. Metab Eng 2002, 4:159-169.
18. 13C-labeled glucose and gas chromatography-mass spectrometry. Metab Eng 2004, 6:340-351.
19. 12) biosynthesis - cloning, expression and crystallisation of the Bacillus megaterium S-adenosyl-l-methionine-dependent cobalt-precorrin-4 transmethylase CbiF. Eur J Biochem 1998, 254:341-346.
20. 12. Philos Trans R Soc Lond B Biol Sci 1976, 273:303-318.