Cobalamin (vitamin B12) biosynthesis - Cloning, expression and crystallisation of the Bacillus megaterium S-adenosyl-l-methionine-dependent cobalt-precorrin-4 transmethylase CbiF

European Journal of Biochemistry

Volumn 254, Issue 2, 1998, Pages 341-346

  Raux, Evelyne ^a   Schubert, Heidi L ^b   Woodcock, Sarah C ^a   Wilson, Keith S ^b   Warren, Martin J ^a,c  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF YORK   (United Kingdom)

^c Department of Molecular Genetics ^*  (United Kingdom)

Author keywords

Bacillus megaterium; CbiF; Cobalt precorrin 4; S adenosyl L methionine; Vitamin B12

Indexed keywords

COBALT PRECORRIN 4 TRANSMETHYLASE; CYANOCOBALAMIN; HISTIDINE; METHYLTRANSFERASE; MUTANT PROTEIN; RECOMBINANT PROTEIN; S ADENOSYLMETHIONINE; UNCLASSIFIED DRUG; COBALT; ESCHERICHIA COLI PROTEIN; PRECORRIN 3 11 METHYLTRANSFERASE; PRECORRIN 4 METHYLTRANSFERASE, E COLI; PRECORRIN-3 11-METHYLTRANSFERASE; PRECORRIN-4 METHYLTRANSFERASE, E COLI; PRIMER DNA;

ARTICLE; BACILLUS MEGATERIUM; BIOSYNTHESIS; CONTROLLED STUDY; CRYSTALLIZATION; ENZYME ACTIVITY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SALMONELLA TYPHIMURIUM; BACTERIAL GENE; DIMERIZATION; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION;

BACILLUS MEGATERIUM; ESCHERICHIA COLI; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

BACILLUS MEGATERIUM; BASE SEQUENCE; CLONING, MOLECULAR; COBALT; CRYSTALLIZATION; DIMERIZATION; DNA PRIMERS; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GENES, BACTERIAL; METHYLTRANSFERASES; MOLECULAR WEIGHT; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; S-ADENOSYLMETHIONINE; VITAMIN B 12;

EID: 0032081276     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2540341.x     Document Type: Article

References (28)

1. 12, (Schneider, Z. & Stroinski, A., eds) pp. 259-266, Walter de Gruyer, Berlin.
2. 12-binding domains of methionine synthase, Science 266, 1669-1674.
3. 12 in Pseudomonas denitrificans, J. Bacteriol. 175, 7430-7440.
4. 12: How the problem of its biosynthesis was solved, Angew. Chem. Int. Ed. Engl. 34, 383-411.
5. Battersby, A. R. (1994) How Nature builds the pigments of life: The conquest of vitamin B12, Science 264, 1551-1557.
6. 12 - a survey of the last four billion years, Angew. Chem. Int. Ed. Engl. 32: 1223-1243.
7. 12) Biosynthetic Genes of Salmonella typhimurium, J. Bacteriol. 175, 3303-3316.
8. 12) biosynthetic operon of Salmonella typhimurium, J. Bacteriol. 174, 24-29.
9. Warren, M. J. & Scott, A. I. (1990) Tetrapyrrole assembly and modification into the ligands of biologically functional cofactors, Trends Biochem. Sci. 15, 486-491.
10. 12 biosynthesis, Angew. Chem. Int. Ed. Engl. 32, 1651-1653.
11. 12 biosynthesis, Heterocycles 39, 471-476.
12. 12) biosynthetic genes; functional studies in S. typhimurium and E. coli, J. Bacteriol. 178, 753-767.
13. 12, J. Chem. Soc. Perkin Trans. I, 1689-1696.
14. 12: Factor IV, a new intermediate in the anaerobic pathway, Proc. Natl Acad. Sci. USA 93, 14316-14319
15. 12) and siroheme biosynthesis, J. Bacteriol. 179, 3202-3212.
16. 12) biosynthesis: Identification and characterization of a Bacillus megaterium cob operon and functional characterization of the genes required to convert uroporphyrinogen III into cobyrinic acid a,c-diamide, Biochem. J., in the press.
17. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning, a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
18. Woodcock, S. C. & Warren, M. J. (1996) Evidence for a covalent enzyme-S-adenosyl-L-methionine complex in the S-adenosyl-L-methionine-dependent transmethylation reaction catalysed by sirohaem synthase, Biochem. J. 313, 415-421.
19. 12 synthesis and propanediol degradation, J. Bacteriol. 174, 2253-2266.
20. Jancarik, J. & Kim, S. H. (1991) Sparse-matrix sampling - a screening method for crystallization of proteins, J. Appl. Crystallogr. 24, 409-411.
21. Macpherson, A. (1982) Preparation and analysis of protein crystals, Wiley, New York.
22. Blanche, F., Debussche, L., Thibaut, D., Crouzet, J. & Cameron, B. (1989) Purification and characterisation of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Pseudomonas denitrificans, J. Bacteriol. 171, 4222-4231.
23. Blanche, F., Robin, C., Couder, M., Faucher, D., Cauchois, L., Cameron, B. & Crouzet, J. (1991) Purification, characterisation, and molecular cloning of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Methanobacterium ivanovii, J. Bacteriol. 173, 4637-4645.
24. Thibaut, D., Couder, M., Crouzet, J., Debussche, L., Cameron, B. & Blanche, F. (1990) Assay and purification of S-adenosyl-L-methioine: precorrin-2 methyltransferase from Pseudomanas denitrificans, J. Bacteriol. 172, 6245-6251.
25. Crouzet, J., Cameron, B., Cauchois, L., Rigault, S., Rouyez, M.-C., Blanche, F., Thibaut, D. & Debussche, L. (1990) Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in the transformation of precorrin-2 to cobyrinic acid, J. Bacteriol. 172, 5980-5990.
26. Warren, M. J., Bolt, E. & Woodcock, S. C. (1994) 5-Ammolaevulinic acid synthase and uroporphyrinogen methylase: two key control enzymes of tetrapyrrole biosynthesis and modification, Ciba Found. Symp. 180, 26-40.
27. Haydock, S. F., Dowson, J. A., Dhillon, N., Roberts, G. A., Cortes, J. & Leadlay, P. F. (1991) Cloning and sequence-analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea - sequence similarities between eryG and a family of S-adenosylmethionine-dependent methyltransferases, Mol. Gen. Genet. 230, 120-128.
28. Yanisch-Perron, C., Viera, J. & Messing, J. (1985) Improved M13 phage cloning vectors and host strains - nucleotide-sequences of the M13mp18 and pUC19 vectors, Gene 33, 103-119.