Expression, purification and characterization of recombinant human serine proteinase inhibitor Kazal-type 6 (SPINK6) in Pichia pastoris

Protein Expression and Purification

Volumn 82, Issue 1, 2012, Pages 144-149

  Lu, Hairong ^a,b   Huang, Jinjiang ^a,b   Li, Guodong ^b   Ge, Kuikui ^a   Wu, Hongyu ^b   Huang, Qingshan ^a,b  

^a FUDAN UNIVERSITY   (China)

^b Shanghai Hi Tech United Bio Technological R and D Co Ltd   (China)

Author keywords

Kallikrein related peptidase 14; Pichia pastoris; Serine proteinase inhibitor Kazal type 6; Yeast

Indexed keywords

KALLIKREIN; KLK14 PROTEIN, HUMAN; LEUKOCYTE ELASTASE INHIBITOR; RECOMBINANT PROTEIN; SPINK6 PROTEIN, HUMAN; TRYPSIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CATTLE; CHEMISTRY; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENETIC TRANSFORMATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; PICHIA;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CHROMATOGRAPHY, GEL; GENE EXPRESSION; HUMANS; KALLIKREINS; PICHIA; PROTEINASE INHIBITORY PROTEINS, SECRETORY; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRANSFORMATION, GENETIC; TRYPSIN;

BOVINAE; ESCHERICHIA COLI; PICHIA PASTORIS;

EID: 84862815266     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.12.004     Document Type: Article

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