An improved HPLC method for the quantitation of 3'-phosphoadenosine 5'-phosphate (PAP) to assay sulfotransferase enzyme activity in HepG2 cells

Journal of Pharmaceutical and Biomedical Analysis

Volumn 62, Issue , 2012, Pages 182-186

  Xu, Jiaojiao ^b   Chen, Ye ^b   Li, Liang ^b   Li, Zaiquan ^b   Wang, Chenai ^b   Zhou, Tianyan ^a,b   Lu, Wei ^a,b  

^a PEKING UNIVERSITY   (China)

^b PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

Author keywords

3' Phosphoadenosine 5' phosphate (PAP); Enzymatic kinetics; HPLC; Sulfotransferases

Indexed keywords

ADENOSINE 3',5' DIPHOSPHATE; METHANOL; PHOSPHATE; SULFOTRANSFERASE; UNCLASSIFIED DRUG; WATER;

ACCURACY; ARTICLE; CELL STRAIN HEPG2; CONTROLLED STUDY; CORRELATION COEFFICIENT; ENZYME ACTIVITY; ENZYME ASSAY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ULTRAVIOLET SPECTROSCOPY;

ADENOSINE DIPHOSPHATE; CALIBRATION; CELL LINE, TUMOR; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; KINETICS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; SULFOTRANSFERASES;

EID: 84862808209     PISSN: 07317085     EISSN: 1873264X     Source Type: Journal    
DOI: 10.1016/j.jpba.2011.12.015     Document Type: Article

References (26)

1. Bowman K.G., Bertozzi C.R. Carbohydrate sulfotransferases: mediators of extracellular communication. Chem. Biol. 1999, 6:R9-R22.
2. Suzuki T., Miki Y., Nakamura Y., Ito K., Sasano H. Steroid sulfatase and estrogen sulfotransferase in human carcinomas. Mol. Cell. Endocrinol. 2011, 340:148-153.
3. Falany C.N. Enzymology of human cytosolic sulfotransferases. FASEB J. 1997, 11:206-216.
4. Bistrup A., Bhakta S., Lee J.K., Belov Y.Y., Gunn M.D., Zuo F.R., Huang C.C., Kannagi R., Rosen S.D., Hemmerich S. Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for l-selectin. J. Cell Biol. 1999, 145:899-910.
5. Hiraoka N., Petryniak B., Nakayama J., Tsuboi S., Suzuki M., Yeh J.C., Izawa D., Tanaka T., Miyasaka M., Lowe J.B., Fukuda M. A novel high endothelial venule-specific sulfotransferase expresses 6-sulfo sialyl Lewis(x), an l-selectin ligand displayed by CD34. Immunity 1999, 11:79-89.
6. Jancova P., Anzenbacher P., Anzenbacherova E. Phase II drug metabolizing enzymes. Biomed. Pap. Med. Fac. Univ. Palacky Olomouc. Czech Repub. 2010, 154:103-116.
7. Surh Y.J. Bioactivation of benzylic and allylic alcohols via sulfo-conjugation. Chem. Biol. Interact. 1998, 109:221-235.
8. Gamage N., Barnett A., Hempel N., Duggleby R.G., Windmill K.F., Martin J.L., McManus M.E. Human sulfotransferases and their role in chemical metabolism. Toxicol. Sci. 2006, 90:5-22.
9. Huang C., Chen Y., Zhou T., Chen G. Sulfation of dietary flavonoids by human sulfotransferases. Xenobiotica 2009, 39:312-322.
10. Westerink W.M., Schoonen W.G., Phase I.I. enzyme levels in HepG2 cells and cryopreserved primary human hepatocytes and their induction in HepG2 cells. Toxicol. In Vitro 2007, 21:1592-1602.
11. Novakova S., Van Dyck S., Glatz Z., Van Schepdael A., Hoogmartens J. Study of enzyme kinetics of phenol sulfotransferase by electrophoretically mediated microanalysis. J. Chromatogr. A 2004, 1032:319-326.
12. Wu Z.L., Ethen C.M., Larson S., Prather B., Jiang W. A versatile polyacrylamide gel electrophoresis based sulfotransferase assay. BMC Biotechnol. 2010, 10:11.
13. Jiang P., Zhou J., Zhang A., Zhong Y. Electrochemical degradation of p-nitrophenol with different processes. J. Environ. Sci. (China) 2010, 22:500-506.
14. Danan L.M., Yu Z., Hoffhines A.J., Moore K.L., Leary J.A. Mass spectrometric kinetic analysis of human tyrosylprotein sulfotransferase-1 and -2. J. Am. Soc. Mass Spectrom. 2008, 19:1459-1466.
15. Pi N., Armstrong J.I., Bertozzi C.R., Leary J.A. Kinetic analysis of NodST sulfotransferase using an electrospray ionization mass spectrometry assay. Biochemistry 2002, 41:13283-13288.
16. Reinen J., Vriese E., Glatt H., Vermeulen N.P. Development and validation of a fluorescence HPLC-based screening assay for inhibition of human estrogen sulfotransferase. Anal. Biochem. 2006, 357:85-92.
17. Hernandez J.S., Watson R.W., Wood T.C., Weinshilboum R.M. Sulfation of estrone and 17 beta-estradiol in human liver. Catalysis by thermostable phenol sulfotransferase and by dehydroepiandrosterone sulfotransferase. Drug Metab. Dispos. 1992, 20:413-422.
18. Hazelton G.A., Hjelle J.J., Dills R.L., Klaassen C.D. A radiometric method for the measurement of adenosine 3'-phosphate 5'-phosphosulfate in rat and mouse liver. Drug Metab. Dispos. 1985, 13:30-34.
19. Wong K.O., Wong K.P. Direct measurement and regulation of 3'-phosphoadenosine 5'-phosphosulfate (PAPS) generation in vitro. Biochem. Pharmacol. 1996, 52:1187-1194.
20. Chen W.T., Liu M.C., Yang Y.S. Fluorometric assay for alcohol sulfotransferase. Anal. Biochem. 2005, 339:54-60.
21. Grum D., van den Boom J., Neumann D., Matena A., Link N.M., Mueller J.W. A heterodimer of human 3'-phospho-adenosine-5'-phosphosulphate (PAPS) synthases is a new sulphate activating complex. Biochem. Biophys. Res. Commun. 2010, 395:420-425.
22. Lin E.S., Yang Y.S. Colorimetric determination of the purity of 3'-phospho adenosine 5'-phosphosulfate and natural abundance of 3'-phospho adenosine 5'-phosphate at picomole quantities. Anal. Biochem. 1998, 264:111-117.
23. Frame L.T., Ozawa S., Nowell S.A., Chou H.C., DeLongchamp R.R., Doerge D.R., Lang N.P., Kadlubar F.F. A simple colorimetric assay for phenotyping the major human thermostable phenol sulfotransferase (SULT1A1) using platelet cytosols. Drug Metab. Dispos. 2000, 28:1063-1068.
24. Food and Drug Administration Guidance for Industry: Biaoanalytical Method Validation 2001, US Department of Health and Human Services, CDER and CVM, Rockville, MD.
25. Hempel N., Barnett A.C., Bolton-Grob R.M., Liyou N.E., McManus M.E. Site-directed mutagenesis of the substrate-binding cleft of human estrogen sulfotransferase. Biochem. Biophys. Res. Commun. 2000, 276:224-230.
26. Chen X., Yang Y., Zheng Y., Martin B.M., Duffel M.W., Jakoby W.B. Tyrosine-ester sulfotransferase from rat liver: Bacterial expression and identification. Protein Expres. Purif. 1992, 3:421-426.