Precise control of repeating unit composition in biodegradable poly(3-hydroxyalkanoate) polymers synthesized by Escherichia coli

Journal of Bioscience and Bioengineering

Volumn 113, Issue 4, 2012, Pages 480-486

  Tappel, Ryan C ^a   Wang, Qin ^a   Nomura, Christopher T ^a  

^a STATE UNIVERSITY OF NEW YORK   (United States)

Author keywords

oxidation; Biodegradable polymers; Escherichia coli; Metabolic engineering; Poly(3 hydroxyalkanoate) (PHA); Repeating unit control

Indexed keywords

CARBON ATOMS; DIFFERENT SIZES; E. COLI; HYDRATASE; METABOLIC ENGINEERING; OXIDATION PATHWAY; PHA SYNTHASES; PHYSICAL CHARACTERISTICS; PLASMID DNA; POLY(3-HYDROXYALKANOATE) (PHA); PRECISE CONTROL; RANDOM MIXTURES; REPEATING UNIT; SHORT CHAIN LENGTHS; SIDE CHAIN LENGTHS; STRUCTURAL COMPOSITION; SUBSTRATE SPECIFICITY;

BIODEGRADABLE POLYMERS; BIOMOLECULES; BIOPOLYMERS; ESCHERICHIA COLI; GENES; OXIDATION; REACTION INTERMEDIATES;

SUBSTRATES;

BIOPOLYMER; ENOYL COENZYME A HYDRATASE; FATTY ACID; PLASMID DNA; POLY(3 HYDROXYALKANOATE); UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; CHEMICAL STRUCTURE; CONTROLLED STUDY; ESCHERICHIA COLI; FADB GENE; FADJ GENE; FATTY ACID OXIDATION; MASS FRAGMENTOGRAPHY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; SYNTHESIS;

ACYLTRANSFERASES; DNA, RECOMBINANT; ENOYL-COA HYDRATASE; ESCHERICHIA COLI; GENE KNOCKOUT TECHNIQUES; PLASMIDS; POLYHYDROXYALKANOATES; TEMPERATURE;

ESCHERICHIA COLI;

EID: 84862803377     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.12.004     Document Type: Article

References (37)

1. Lu J., Tappel R.C., Nomura C.T. Mini-review: biosynthesis of poly(hydroxyalkanoates). Pol. Rev. 2009, 49:226-248.
2. Sudesh K., Abe H., Doi Y. Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Prog. Polym. Sci. 2000, 25:1503-1555.
3. Abe H., Doi Y. Side-chain effect of second monomer units on crystalline morphology, thermal properties, and enzymatic degradability for random copolyesters of (R)-3-hydroxybutyric acid with (R)-3-hydroxyalkanoic acids. Biomacromolecules 2002, 3:133-138.
4. Zhu C., Nomura C.T., Perrotta J.A., Stipanovic A.J., Nakas J.P. Production and characterization of poly-3-hydroxybutyrate from biodiesel-glycerol by Burkholderia cepacia ATCC 17759. Biotechnol. Prog. 2010, 26:424-430.
5. Steinbuuchel A., Valentin H.E., Schounebaum A. Application of recombinant gene technology for production of polyhydroxyalkanoic acids: biosynthesis of poly(4-hydroxybutyric acid) homopolyester. J. Polym. Environ. 1994, 2:67-74.
6. Wang H.-H., Li X.-T., Chen G.-Q. Production and characterization of homopolymer polyhydroxyheptanoate (P3HHp) by a fadBA knockout mutant Pseudomonas putida KTOY06 derived from P. putida KT2442. Process Biochem. 2009, 44:106-111.
7. Liu Q., Luo G., Zhou X.R., Chen G.-Q. Biosynthesis of poly(3-hydroxydecanoate) and 3-hydroxydodecanoate dominating polyhydroxyalkanoates by β-oxidation pathway inhibited Pseudomonas putida. Metab. Eng. 2011, 13:11-17.
8. Wang H.-H., Zhou X.-R., Liu Q., Chen G.-Q. Biosynthesis of polyhydroxyalkanoate homopolymers by Pseudomonas putida. Appl. Microbiol. Biotechnol. 2011, 89:1497-1507.
9. Rai R., Yunos D.M., Boccaccini A.R., Knowles J.C., Barker I.A., Howdle S.M., Tredwell G.D., Keshavarz T., Roy I. Poly-3-hydroxyoctanoate P(3HO), a medium chain length polyhydroxyalkanoate homopolymer from Pseudomonas mendocina. Biomacromolecules 2011, 12:2126-2136.
10. Rai R., Keshavarz T., Roether J.A., Boccaccini A.R., Roy I. Medium chain length polyhydroxyalkanoates, promising new biomedical materials for the future. Mater. Sci. Eng. R 2011, 72:29-47.
11. Campbell J.W., Morgan-Kiss R.M., Cronan J.E. A new Escherichia coli metabolic competency: growth on fatty acids by a novel anaerobic β-oxidation pathway. Mol. Microbiol. 2003, 47:793-805.
12. + Escherichia coli. Appl. Environ. Microbiol. 1995, 61:2487-2492.
13. Ren Q., Sierro N., Kellerhals M., Kessler B., Witholt B. Properties of engineered poly-3-hydroxyalkanoates produced in recombinant Escherichia coli strains. Appl. Environ. Microbiol. 2000, 66:1311-1320.
14. Fiedler S., Steinbüchel A., Rehm B.H. The role of the fatty acid β-oxidation multienzyme complex from Pseudomonas oleovorans in polyhydroxyalkanoate biosynthesis: molecular characterization of the fadBA operon from P. oleovorans and of the enoyl-CoA hydratase genes phaJ from P. oleovorans and Pseudomonas putida. Arch. Microbiol. 2002, 178:149-160.
15. Snell K.D., Feng F., Zhong L., Martin D., Madison L.L. YfcX enables medium-chain-length poly(3-hydroxyalkanoate) formation from fatty acids in recombinant Escherichia coli fadB strains. J. Bacteriol. 2002, 184:5696-5705.
16. Park S.J., Park J.P., Lee S.Y., Doi Y. Enrichment of specific monomer in medium-chain-length poly(3-hydroxyalkanoates) by amplification of fadD and fadE genes in recombinant Escherichia coli. Enzyme Microb. Technol. 2003, 33:62-70.
17. DiRusso C.C., Heimert T.L., Metzger A.K. Characterization of FadR, a global transcriptional regulator of fatty acid metabolism in Escherichia coli. Interaction with the fadB promoter is prevented by long chain fatty acyl coenzyme As. J. Biol. Chem. 1992, 267:8685-8691.
18. Spratt S.K., Ginsburgh C.L., Nunn W.D. Isolation and genetic characterization of Escherichia coli mutants defective in propionate metabolism. J. Bacteriol. 1981, 146:1166-1169.
19. Pauli G., Overath P. ato Operon: a highly inducible system for acetoacetate and butyrate degradation in Escherichia coli. Eur. J. Biochem. 1972, 29:553-562.
20. Jenkins L.S., Nunn W.D. Genetic and molecular characterization of the genes involved in short-chain fatty acid degradation in Escherichia coli: the ato system. J. Bacteriol. 1987, 169:42-52.
21. Tsuge T., Taguchi K., Taguchi S., Doi Y. Molecular characterization and properties of (R)-specific enoyl-CoA hydratases from Pseudomonas aeruginosa: metabolic tools for synthesis of polyhydroxyalkanoates via fatty acid β-oxidation. Int. J. Biol. Macromol. 2003, 31:195-205.
22. Sato S., Kanazawa H., Tsuge T. Expression and characterization of (R)-specific enoyl coenzyme A hydratases making a channeling route to polyhydroxyalkanoate biosynthesis in Pseudomonas putida. Appl. Microbiol. Biotechnol. 2011, 90:951-959.
23. Fukui T., Yokomizo S., Kobayashi G., Doi Y. Co-expression of polyhydroxyalkanoate synthase and (R)-enoyl-CoA hydratase genes of Aeromonas caviae establishes copolyester biosynthesis pathway in Escherichia coli. FEMS Microbiol. Lett. 1999, 170:69-75.
24. Tsuge T., Fukui T., Matsusaki H., Taguchi S., Kobayashi G., Ishizaki A., Doi Y. Molecular cloning of two (R)-specific enoyl-CoA hydratase genes from Pseudomonas aeruginosa and their use for polyhydroxyalkanoate synthesis. FEMS Microbiol. Lett. 2000, 184:193-198.
25. Budde C.F., Riedel S.L., Willis L.B., Rha C., Sinskey A.J. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl. Environ. Microbiol. 2011, 77:2847-2854.
26. Takase K., Taguchi S., Doi Y. Enhanced synthesis of poly(3-hydroxybutyrate) in recombinant Escherichia coli by means of error-prone PCR mutagenesis, saturation mutagenesis, and in vitro recombination of the type II polyhydroxyalkanoate synthase gene. J. Biochem. 2003, 133:139-145.
27. Matsusaki H., Manji S., Taguchi K., Kato M., Fukui T., Doi Y. Cloning and molecular analysis of the poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. strain 61-3. J. Bacteriol. 1998, 180:6459-6467.
28. Nomura C.T., Tanaka T., Gan Z., Kuwabara K., Abe H., Takase K., Taguchi K., Doi Y. Effective enhancement of short-chain-length-medium-chain-length polyhydroxyalkanoate copolymer production by coexpression of genetically engineered 3-ketoacyl-acyl-carrier-protein synthase III (fabH) and polyhydroxyalkanoate synthesis genes. Biomacromolecules 2004, 5:1457-1464.
29. Nomura C.T., Tanaka T., Eguen T.E., Appah A.S., Matsumoto K., Taguchi S., Ortiz C.L., Doi Y. FabG mediates polyhydroxyalkanoate production from both related and nonrelated carbon sources in recombinant Escherichia coli LS5218. Biotechnol. Prog. 2008, 24:342-351.
30. Datsenko K.A., Wanner B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA 2000, 97:6640-6645.
31. Wang Q., Nomura C.T. Monitoring differences in gene expression levels and polyhydroxyalkanoate (PHA) production in Pseudomonas putida KT2440 grown on different carbon sources. J. Biosci. Bioeng. 2010, 110:653-659.
32. Sambrook J.J., Russell D.W. Molecular cloning: A laboratory manual 2001, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
33. Jiang X., Ramsay J.A., Ramsay B.A. Acetone extraction of mcl-PHA from Pseudomonas putida KT2440. J. Microbiol. Methods 2006, 67:212-219.
34. Arai Y., Nakashita H., Suzuki Y., Kobayashi Y., Shimizu T., Yasuda M., Doi Y., Yamaguchi I. Synthesis of a novel class of polyhydroxyalkanoates in Arabidopsis peroxisomes, and their use in monitoring short-chain-length intermediates of β-oxidation. Plant Cell Physiol. 2002, 43:555-562.
35. Matsusaki H., Abe H., Taguchi K., Fukui T., Doi Y. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant bacteria expressing the PHA synthase gene phaC1 from Pseudomonas sp. 61-3. Appl. Microbiol. Biotechnol. 2000, 53:401-409.
36. Sparks J., Scholz C. Synthesis and characterization of a cationic poly(β-hydroxyalkanoate). Biomacromolecules 2008, 9:2091-2096.
37. Matsumoto K., Takase K., Aoki E., Doi Y., Taguchi S. Synergistic effects of Glu130Asp substitution in the type II polyhydroxyalkanoate (PHA) synthase: enhancement of PHA production and alteration of polymer molecular weight. Biomacromolecules 2005, 6:99-104.