Multiplex time-reducing quantitative polymerase chain reaction assay for determination of telomere length in blood and tissue DNA

Analytical and Bioanalytical Chemistry

Volumn 403, Issue 1, 2012, Pages 157-166

  Jiao, Jingjing ^b   Kang, Jing X ^b   Tan, Rui ^c   Wang, Jingdong ^b   Zhang, Yu ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c SOUTHWEST JIAOTONG UNIVERSITY   (China)

Author keywords

Cancer; Genomic DNA; Multiplex qPCR; Telomere length; Time reducing

Indexed keywords

CANCER; GENOMIC DNA; MULTIPLEX QPCR; TELOMERE LENGTH; TIME-REDUCING;

DISEASES; GENES; MAMMALS; MULTIPLEXING; POLYMERASE CHAIN REACTION; TUMORS;

DNA;

DNA; PRIMER DNA;

ANIMAL; ARTICLE; BLOOD; GENETICS; METABOLISM; METHODOLOGY; MOUSE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; TELOMERE;

ANIMALS; BASE SEQUENCE; DNA; DNA PRIMERS; MICE; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; TELOMERE;

MUS;

EID: 84862802454     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-5783-3     Document Type: Article

References (42)

1. MA Blasco 2005 Telomeres and human disease: ageing, cancer and beyond Nat Rev Genet 6 611 622 10.1038/nrg1656 1:CAS:528:DC%2BD2MXntFeqtbw%3D
2. S Bekaert T de Meyer P van Oostveldt 2005 Telomere attrition as ageing biomarker Anticancer Res 25 3011 3021 1:CAS:528:DC%2BD2MXpt1WrtLg%3D
3. RT Calado NS Young 2009 Telomere diseases N Engl J Med 361 2353 2365 10.1056/NEJMra0903373 1:CAS:528:DC%2BD1MXhsFGqsrbO
4. KA Mather AF Jorm RA Parslow H Christensen 2011 Is telomere length a biomarker of aging? A review. J Gerontol Ser A-Biol Sci Med Sci 66 202 213
5. H Oeseburg RA de Boer WH van Gilst P van der Harst 2010 Telomere biology in healthy aging and disease Pflugers Arch 459 259 268 10.1007/s00424-009-0728-1 1:CAS:528:DC%2BC3cXisVSksQ%3D%3D
6. G von Figura D Hartmann ZF Song KL Rudolph 2009 Role of telomere dysfunction in aging and its detection by biomarkers J Mol Med 87 1165 1171 10.1007/s00109-009-0509-5
7. VA Zakian 2009 The ends have arrived Cell 139 1038 1040 10.1016/j.cell.2009.11.033 1:CAS:528:DC%2BC3cXhslarsbw%3D
8. RC Allshire M Dempster ND Hastie 1989 Human telomeres contain at least three types of G-rich repeat distributed non-randomly Nucleic Acids Res 17 4611 4627 10.1093/nar/17.12.4611 1:CAS:528:DyaL1MXkvFWmt7c%3D
9. M Kimura RC Stone SC Hunt J Skurnick XB Lu XJ Cao CB Harley A Aviv 2010 Measurement of telomere length by the Southern blot analysis of terminal restriction fragment lengths Nat Protoc 5 1596 1607 10.1038/nprot.2010.124 1:CAS:528:DC%2BC3cXht1aksLnN
10. K Oexle 1998 Telomere length distribution and southern blot analysis J Theor Biol 190 369 377 10.1006/jtbi.1997.0559 1:STN:280:DyaK1c3gt1Grug%3D%3D
11. D Norwood DS Dimitrov 1998 Sensitive method for measuring telomere lengths by quantifying telomeric DNA content of whole cells Biotechniques 25 1040 1045 1:CAS:528:DyaK1MXjtVKi
12. RM Cawthon 2002 Telomere measurement by quantitative PCR Nucleic Acids Res 30 e47 10.1093/nar/30.10.e47
13. RJ Callicott JE Womack 2006 Real-time PCR assay for measurement of mouse telomeres Comp Med 56 17 22 1:CAS:528:DC%2BD28XhslCgtb0%3D
14. J Lin E Epel J Cheon C Kroenke E Sinclair M Bigos W Owen M Synthia E Blackburn 2010 Analyses and comparisons of telomerase activity and telomere length in human T and B cells: Insights for epidemiology of telomere maintenance J Immunol Methods 352 71 80 10.1016/j.jim.2009.09.012 1:CAS:528: DC%2BD1MXhsFyns7nM
15. RM Cawthon 2009 Telomere length measurement by a novel monochrome multiplex quantitative PCR method Nucleic Acids Res 37 e21 10.1093/nar/gkn1027
16. ME Gil TL Coetzer 2004 Real-time quantitative PCR of telomere length Mol Biotechnol 27 169 172 10.1385/MB:27:2:169 1:CAS:528:DC%2BD2cXlsFGmsLc%3D
17. MT Akbari M Noruzinia H Mozdarani M Hamid 2011 Determination of exon 7 SMN1 deletion in Iranian patients and heterozygous carriers by quantitative real-time PCR J Genet 90 133 136 10.1007/s12041-011-0038-1
18. VC Sheffield DR Cox LS Lerman RM Myers 1989 Attachment of a 40-base-pair G∈+∈C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes Proc Natl Acad Sci U S A 86 232 236 10.1073/pnas.86.1.232 1:CAS:528: DyaL1MXhtV2nsrY%3D
19. Innis MA, Gelfand DH (1990) In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols. A guide to methods and applications. Academic Press, San Diego, pp 3-13
20. O Henegariu NA Heerema SR Dlouhy GH Vance PH Vogt 1997 Multiplex PCR: Critical parameters and step-by-step protocol Biotechniques 23 504 511 1:CAS:528:DyaK2sXmtVSnsrw%3D
21. VK Viswanathan K Krcmarik NP Cianciotto 1999 Template secondary structure promotes polymerase jumping during PCR amplification Biotechniques 27 508 511 1:CAS:528:DyaK1MXlvVKjs7c%3D
22. J Kang MS Lee DG Gorenstein 2005 The enhancement of PCR amplification of a random sequence DNA library by DMSO and betaine: Application to in vitro combinatorial selection of aptamers J Biochem Biophys Methods 64 147 151 10.1016/j.jbbm.2005.06.003 1:CAS:528:DC%2BD2MXos1Wrsbw%3D
23. R Chakrabarti CE Schutt 2002 Novel sulfoxides facilitate GC-rich template amplification Biotechniques 32 870 872
24. N Baskaran RP Kandpal AK Bhargava MW Glynn A Bale SM Weissmann 1996 Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content Genome Res 6 633 638 10.1101/gr.6.7.633 1:CAS:528:DyaK28XksFOnsLs%3D
25. W Henke K Herdel K Jung D Schnorr SA Loening 1997 Betaine improves the PCR amplification of GC-rich DNA sequences Nucleic Acids Res 25 3957 3958 10.1093/nar/25.19.3957 1:CAS:528:DyaK2sXmsF2ntrc%3D
26. T Weissensteiner JS Lanchbury 1996 Strategy for controlling preferential amplification and avoiding false negatives in PCR typing reactions Biotechniques 21 1102 1108 1:CAS:528:DyaK28XntlGgsrk%3D
27. FC Lawyer S Stoffel RK Saiki SY Chang PA Landre RD Abramson DH Gelfand 1993 High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5′ to 3′ exonuclease activity PCR Methods Appl 2 275 287 1:CAS:528:DyaK3sXksVeqsb4%3D
28. Y Doki K Murakami T Yamaura S Sugiyama T Misaki I Saiki 1999 Mediastinal lymph node metastasis model by orthotopic intrapulmonary implantation of Lewis lung carcinoma cells in mice Br J Cancer 79 1121 1126 10.1038/sj.bjc.6690178 1:STN:280:DyaK1M7ps1ersw%3D%3D
29. X Qu G-J Zheng F-L Liu D Zhang J Zhang M-X Yang L-Y Xia 2004 Experimental research on anti-tumor metastasis effect of basil polysaccharide in vivo Chin J Integr Med 10 138 140 10.1007/BF02836389 1:CAS:528:DC%2BD2MXjsFSnsb8%3D
30. CW Greider 1996 Telomere length regulation Annu Rev Biochem 65 337 365 10.1146/annurev.bi.65.070196.002005 1:CAS:528:DyaK28XktFamtLY%3D
31. 3 repeats Proc Natl Acad Sci U S A 94 7423 7428 10.1073/pnas.94.14.7423 1:CAS:528:DyaK2sXksFOmu78%3D
32. MM Gramatges AA Bertuch 2010 Measuring relative telomere length: Is tissue an issue? Aging 2 756 757
33. MA Blasco HW Lee MP Hande E Samper PM Lansdorp RA DePinho CW Greider 1997 Telomere shortening and tumor formation by mouse cells lacking telomerase RNA Cell 91 25 34 10.1016/S0092-8674(01)80006-4 1:CAS:528:DyaK2sXmslGgt7w%3D
34. T von Zglinicki R Pilger N Sitte 2000 Accumulation of single-strand breaks is the major cause of telomere shortening in human fibroblasts Free Radic Biol Med 28 64 74 10.1016/S0891-5849(99)00207-5
35. SE Artandi S Chang SL Lee S Alson GJ Gottlieb L Chin RA DePinho 2000 Telomere dysfunction promotes non-reciprocal translocations and epithelial cancers in mice Nature 406 641 645 10.1038/35020592 1:STN:280: DC%2BD3cvjtVKrug%3D%3D
36. AM Joshua B Vukovic I Braude S Hussein M Zielenska J Srigley A Evans JA Squire 2007 Telomere attrition in isolated high-grade prostatic intraepithelial neoplasia and surrounding stroma is predictive of prostate cancer Neoplasia 9 81 89 10.1593/neo.06745 1:CAS:528:DC%2BD2sXhvFChtL0%3D
37. HX Ma ZY Zhou S Wei ZS Liu KA Pooley AM Dunning U Svenson G Roos HD Hosgood M Shen QY Wei 2011 Shortened telomere length is associated with increased risk of cancer: A meta-analysis PLoS One 6 e20466 10.1371/journal. pone.0020466 1:CAS:528:DC%2BC3MXnvVSjur0%3D
38. IM Wentzensen L Mirabello RM Pfeiffer SA Savage 2011 The association of telomere length and cancer: A meta-analysis Cancer Epidemiol Biomarkers Prev 20 1238 1250 10.1158/1055-9965.EPI-11-0005 1:CAS:528:DC%2BC3MXnt1aksbg%3D
39. P Willeit J Willeit A Mayr S Weger F Oberhollenzer A Brandstätter F Kronenberg S Kiechl 2010 Telomere length and risk of incident cancer and cancer mortality JAMA-J Am Med Assoc 304 69 75 10.1001/jama.2010.897 1:CAS:528:DC%2BC3cXovVSjurc%3D
40. P Thomas NJ O' Callaghan M Fenech 2008 Telomere length in white blood cells, buccal cells and brain tissue and its variation with ageing and Alzheimer's disease Mech Ageing Dev 129 183 190 10.1016/j.mad.2007.12.004 1:CAS:528:DC%2BD1cXislaiur8%3D
41. NJ O' Callaghan M Fenech 2011 A quantitative PCR method for measuring absolute telomere length Biol Proced Online 13 3 10.1186/1480-9222-13-3
42. Canela A, Klatt P, Blasco MA (2007) In: Tollefsbol TO (ed) Methods in Molecular Biology: Biological aging: Methods and protocols. Humana Press Inc., Towana, NJ