Sensitive method for measuring telomere lengths by quantifying telomeric DNA content of whole cells

BioTechniques

Volumn 25, Issue 6, 1998, Pages 1040-1045

  Norwood, David ^a   Dimitrov, Dimiter S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DNA;

ACCURACY; ANIMAL CELL; ARTICLE; CHIMPANZEE; DNA CONTENT; DNA PURIFICATION; GENE ISOLATION; HUMAN; HUMAN CELL; NONHUMAN; TELOMERE;

ANIMALIA; PAN TROGLODYTES;

EID: 0032432352     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98256cr02     Document Type: Article

References (8)

1. Allsopp, R.C., H. Vaziri, C. Patterson, S. Goldstein, E.V. Younglai, A.B. Futcher, C.W. Greider and C.B. Harley. 1992. Telomere length predicts replicative capacity of human fibroblasts. Proc. Natl. Acad. Sci. USA. 89:10114-10118.
2. Bryant, J.E., K.G. Hutchings, R.K. Moyzis and J.K. Griffith. 1997. Measurement of telomeric DNA content in human tissues. BioTechniques 23:476-484.
3. Feng, Y., D. Norwood, R. Shibata, D. Gee, X. Xaio, M. Martin, S. Zeichner and D. Dimitrov. Telomere dynamics in HIV-1 infected and uninfected chimpanzees measured by an improved method based on high-resolution two-dimensional calibration of DNA sizes. J. Med. Primatol. (In press).
4. Harley, C.B., A.B. Futcher and C.W. Greider. 1990. Telomeres shorten during aging of human fibroblasts. Nature 345:458-460.
5. Harley, C.B., H. Vaziri, C.M. Counter and R.C. Allsopp. 1992. The telomere hypothesis of cellular aging. Exp. Gerontol. 27:375-382.
6. Vaziri, H., W. Dragowska, R.C. Allsopp, T.E. Thomas, C.B. Harley and P.M. Lansdorp. 1994. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age. Proc. Natl. Acad. Sci. USA 91:9857-9860.
7. Vaziri, H., F. Schachter, I. Uchida, L. Wei, X. Zhu, R. Effros, D. Cohen and C.B. Harley. 1993. Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes. Am. J. Hum. Genet. 52:661-667.
8. Zakian, V.A. 1989. Structure and function of telomeres. Annu. Rev. Genet. 23:579-604.