Detecting S-adenosyl-l-methionine-induced conformational change of a histone methyltransferase using a homogeneous time-resolved fluorescence-based binding assay

Analytical Biochemistry

Volumn 423, Issue 1, 2012, Pages 171-177

  Lin, Ying ^a   Fan, Hong ^a   Frederiksen, Mathias ^b   Zhao, Kehao ^a   Jiang, Lei ^a   Wang, Zhaofu ^a   Zhou, Shaolian ^a   Guo, Weihui ^a   Gao, Jun ^a   Li, Shu ^c   Harrington, Edmund ^c   Meier, Peter ^b   Scheufler, Clemens ^b   Xu, Yao Chang ^a   Atadja, Peter ^a   Lu, Chris ^a   Li, En ^a   Justin Gu, X ^a  

^a Novartis Institutes for Biomedical Research   (China)

^b NOVARTIS PHARMA AG   (Switzerland)

^c NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

BIX 01294; CJP702; Conformational change; G9a; HMT; SAM; SET domain; Sinefungin; Uncompetitive inhibitor

Indexed keywords

AMINO ACIDS; ENZYMES; FLUORESCENCE;

BIX-01294; CJP702; CONFORMATIONAL CHANGE; SET DOMAIN; SINEFUNGIN; UNCOMPETITIVE INHIBITOR;

PEPTIDES;

BIOTIN DERIVATIVE; HISTONE METHYLTRANSFERASE; S ADENOSYLHOMOCYSTEINE; S ADENOSYLMETHIONINE; SINEFUNGIN;

ARTICLE; CONFORMATIONAL TRANSITION; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME INHIBITION; ENZYME INHIBITOR INTERACTION; ENZYME KINETICS; FLUORESCENCE ANALYSIS; HUMAN; PRIORITY JOURNAL;

EID: 84862800655     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.01.019     Document Type: Article

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