Optimization and development of capillary electrophoresis for separating and identifying wheat low molecular weight glutenin subunits

Journal of Cereal Science

Volumn 55, Issue 2, 2012, Pages 254-256

  Li, Jing ^a   Wang, Shunli ^a   Yu, Zitong ^a   Li, Xiaohui ^a   Guo, Guangfang ^a   Feng, Shuo ^a   Ma, Wujun ^b   Yan, Yueming ^a  

^a CAPITAL NORMAL UNIVERSITY   (China)

^b MURDOCH UNIVERSITY   (Australia)

Author keywords

Capillary electrophoresis; LMW GS; SDS PAGE; Triticum aestivum L.

Indexed keywords

TRITICUM AESTIVUM;

EID: 84862798778     PISSN: 07335210     EISSN: 10959963     Source Type: Journal    
DOI: 10.1016/j.jcs.2011.12.005     Document Type: Article

References (11)

1. Bean S.R., Tilley M. Separation of water-soluble proteins from cereals by high-performance capillary electrophoresis (HPCE). Cereal Chemistry 2003, 80:505-510.
2. Bietz J.A., Schmalzried E. Capillary electrophoresis of wheat gliadin: initial studies and application to varietal identification. LWT-Food Science and Technology 1995, 28:174-184.
3. Gao L., Ma W.J., Chen J., Wang K., Li J., Wang S.L., Bekes F., Appels R., Yan Y. Characterization and comparative analysis of wheat high molecular weight glutenin subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS. Journal of Agriculture and Food Chemistry 2010, 58:2777-2786.
4. Lookhart G.L., Bean S.R. Improvements in cereal protein separations by capillary electrophoresis: resolution and reproducibility. Cereal Chemistry 1996, 73:81-87.
5. Melas V., Morel M.-H., Autran I.-C., Feillet P. Simple and rapid method for purifying low molecular weight subunits of glutenin from wheat. Cereal Chemistry 1994, 71:234-237.
6. Miguel H., García-Cabañas V., Simo C., Cifuente A. Recent advances in the application of capillary electromigration methods for food analysis and foodomics. Electrophoresis 2010, 31:205-228.
7. r wheat proteins. II. Effects on Dough Properties Journal of Cereal Science 1995, 21:117-126.
8. t gliadin loci in Aegilops tauschii as revealed by acid polyacrylamide gel and capillary electrophoresis. Plant Breeding 2003, 122:120-124.
9. Yan Y., Jiang Y., Sun M., Yu J., Xiao Y., Zheng J., Hu Y., Cai M., Li Y., Hsam S.L.K., Zeller F.J. Rapid identification of HMW glutenin subunits from different hexaploid wheat species by acidic capillary electrophoresis. Cereal Chemistry 2004, 81:561-566.
10. t1 glutenin gene of Aegilops tauschii. Theoretical and Applied Genetics 2004, 108:1349-1358.
11. Zhang Q., Dong Y., An X., Wang A., Zhang Y., Li X., Xia X., He Z., Yan Y. Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS). Journal of Cereal Science 2008, 47:252-261.