Improvements in cereal protein separations by capillary electrophoresis: Resolution and reproducibility

Cereal Chemistry

Volumn 73, Issue 1, 1996, Pages 81-87

  Lookhart, George L ^a   Bean, Scott R ^b  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b KANSAS STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRITICAL MICELLE CONCENTRATION;

BUFFER COMPOSITION; BUFFER PH; CEREAL PROTEINS; CLEANING PROTOCOLS; HYDROXYPROPYL METHYLCELLULOSE; OPTIMAL RESOLUTION; PHOSPHATE BUFFERS; PROTEIN SEPARATIONS; PROTOCOL COMPOSITION; REPRODUCIBILITIES;

CAPILLARY ELECTROPHORESIS;

TRITICUM AESTIVUM;

EID: 0030512421     PISSN: 00090352     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. BAKHELLA, M., LOOKHART, G. L., and HOSENEY, R. C. 1991. Identification of Moroccan wheats by reversed-phase high performance liquid chromatography and electrophoretic procedures. Actes Inst. Agron. Vet. (Maroc) 11:5.
2. BAKHELLA, M., LOOKHART, G. L., and HOSENEY, R. C. 1992. Identification of Moroccan oat cultivars by electrophoresis of their prolamins and total proteins. Actes Inst. Agron. Vet. (Maroc) 12:19.
3. BIETZ, J. A. 1983. Separation of cereal proteins by reversed-phase highperformance liquid chromatography. J. Chromatogr. 255:219.
4. BIETZ, J. A., and LOOKHART, G. L. 1994. Wheat varietal identification by capillary electrophoresis: An inter-laboratory comparison of methods. (Abstr.) Cereal Foods World 39:603.
5. BIETZ, J. A., and SCHMALZRIED, E. 1995. Capillary electrophoresis of wheat gliadin: Initial studies and application to varietal identification. Food Sci. Technol. (Zur.) 28:174.
6. CHEN, F. A. 1991. Rapid protein analysis by capillary electrophoresis. J. Chromatogr. 559:445.
7. GORDON, M. J., LEE, K., ARIAS, A. A., and ZARE, R. N. 1991. Protocol for resolving protein mixtures in capillary zone electrophoresis. Anal. Chem. 63:69.
8. GREVE, K. F, NASHABEH, W., and KARGER, B. L. 1994. Use of zwitterionic detergents for the separation of closely related peptides by capillary electrophoresis. J. Chromatogr, 680:15.
9. HEISEL, S. E., PETERSON, D. M., and JONES, B. L. 1986. Identification of United States barley cultivais by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of hordeins. Cereal Chem. 63:500.
10. HUEBNER, F. R., and BIETZ, J. A. 1994. RP-HPLC for varietal identification in cereals and legumes. Pages 97-120 in: High-Performance Liquid Chromatography of Cereal and Legume Proteins: Wheat. J. E. Kruger and J. A. Bietz, eds. Am. Assoc. Cereal Chem.: St. Paul, MN.
11. HUEBNER, F. R., HUSSAIN, A., LOOKHART, G. L., BIETZ, J. A., BUSHUK, W., and JULIANO, B. O. 1991. Discrimination of sister line IR rice varieties by polyacrylamide gel electrophoresis and reversed-phase high-performance liquid chromatography. Cereal Chem. 68:583.
12. KENNDLER, E. 1993. Organic solvents in capillary electrophoresis. Pages 161-186 in: Capillary Electrophoresis Technology. N. A. Guzmon, ed. Marcel Dekker Inc.: New York.
13. KOHR, J., and ENGELHARDT, H. 1991. Capillary electrophoresis with surface coated capillaries. J. Microcolumn Sep. 3:491.
14. LOOKHART, G. L. 1985. Identification of oat cultivars by combining polyacrylamide gel electrophoresis and reversed-phase high-performance liquid chromatography. Cereal Chem. 62:345.
15. LOOKHART, G L. 1990. Cereal proteins: Composition of their major fractions and methods for identification. Pages 441-468 in: Handbook of Cereal Science and Technology. K. J. Lorenz and K. Kulp, eds. Marcel Dekker, Inc.: New York.
16. LOOKHART, G. L., and ALBERS, L. D. 1988. Correlations between reversed-phase high-performance liquid chromatography and acid-and sodium dodecyl sulfate-polyacrylamide gel electrophoretic data on prolamins from wheat sister lines differing widely in baking quality. Cereal Chem. 65:222.
17. LOOKHART, G. L., and BEAN, S. R. 1995a. A fast method for wheat cultivar differentiation using capillary zone electrophoresis. Cereal Chem. 72:42.
18. LOOKHART, G. L., and BEAN, S. R. 1995b. Rapid differentiation of oat cultivars and of rice cultivars by capillary zone electrophoresis. Cereal Chem. 72:312.
19. LOOKHART, G. L., and BEAN, S. R. 1995c. Separation and characterization of wheat protein fractions by high-performance capillary electrophoresis. Cereal Chem. 72:527.
20. LOOKHART, G. L., and JULIANO, B. O. 1994. RP-HPLC for varietal identification in cereals and legumes: Rice. Pages 175-183 in: High-Performance Liquid Chromatography of Cereal and Legume Proteins. J. E. Kruger and J. A. Bietz, eds. Am. Assoc. of Cereal Chem.: St. Paul, MN.
21. LOOKHART, G. L., and POMERANZ, Y. 1985. Characterization of oat species by polyacrylamide gel electrophoresis and high-performance liquid chromatography of their prolamin proteins. Cereal Chem. 62:162.
22. LOOKHART, G. L., and WRIGLEY, C. W. 1995. Variety identification by electrophoretic analysis. Pages 55-71 in: Identification of Food Grain Varieties. C. W. Wrigley, ed. Am. Assoc. of Cereal Chem.: St. Paul, MN.
23. LOOKHART, G. L., JONES, B. L., HALL, S. B., and FINNEY, K. F. 1982. An improved method for standardizing polyacrylamide gel electrophoresis of wheat gliadin proteins. Cereal Chem. 59:178.
24. LOOKHART, G. L., ALBERS, L. D., and BIETZ, J. A. 1986. A Comparison of PAGE and HPLC analyses of gliadin polymorphism in the wheat cultivar Newton. Cereal Chem. 63:497.
25. LOOKHART, G. L., ALBERS, L. D., POMERANZ, Y., and WEBB, B. D. 1987. Identification of U.S. rice cultivars by high-performance liquid chromatography. Cereal Chem. 64:199.
26. LOOKHART, G. L., JULIANO, B. O., and WEBB, B. D. 1991. The effect of solvent extraction, environment, and genetic background on differentiating milled rice by reversed-phase high-performance liquid chromatography. Cereal Chem. 68:396.
27. LOOKHART, G. L., BEAN, S. R., and AKKINA, S. K. 1994. High performance capillary electrophoresis (HPCE) optimization for wheat gliadin analyses and comparison of individual protein peak positions to high performance liquid chromatography (HPLC) peaks. (Abstr.) Cereal Foods World 39:627.
28. LOOKHART, G. L., BEAN, S. R., GRAYBOSCH, R. A., and CHUNG, O. K. 1995. Differentiation of 1AL/1RS from 1BL/1RS wheat-rye translocation lines by capillary electrophoresis. (Abstr.) Cereal Foods World 40:651.
29. MARCHYLO, B. A., and LABERGE, D. E. 1980. Barley cultivar identification by electrophoretic analysis of hordein proteins. Extraction and separation of hordein proteins and environmental effects. Can. J. Plant Sci. 60:1343.
30. MARCHYLO, B. A., HATCHER, D. W., and KRUGER, J. E. 1988. Identification of wheat cultivars by reversed-phase high-performance liquid chromatography of storage proteins. Cereal Chem. 65:28.
31. McCORMICK, R. M. 1988. Capillary zone electrophoretic separation of peptides and proteins using low pH buffers in modified silica capillaries. Anal. Chem. 60:2322.
32. MITSYUK, B. M. 1972. Mechanism of the reaction of silica with phosphoric acid in aqueous solutions. Russ. J. Inorg. Chem. (Engl. transl.) 17:471.
33. MORING, S. E., and NOLAN, J. A. 1990. Hydrophobic interaction capillary electrophoresis of peptides and proteins. Appl. Biosystems Inc. Res. News 2.
34. ODA, R. P., and LANDERS, J. P. 1994. Introduction to capillary electrophoresis. Pages 9-42 in: Handbook of Capillary Electrophoresis. J. P. Landers, ed. CRC Press: Boca Raton, FL.
35. SAPIRSTEIN, H. D., SCANLON, M. G., and BUSHUK, W. 1989. Normalization of high-performance liquid chromatography peak retention times of computerized comparison of wheat prolamin chromatograms. J. Chromatogr. 469:127.
36. SCHWER, C., and KENNDLER, E. 1991. Electrophoresis in fused-silica capillaries: The influence of organic solvents on the electroosmotic velocity and the ζ potential. Anal. Chem. 63:1801.
37. SMITH, S. C., STRASTERS, J. K., and KHALEDI, M. G. 1991. Influence of operating parameters on reproducibility in capillary electrophoresis. J. Chromatogr. 559:57.
38. STRICKLAND, M., and STRICKLAND, N. 1990. Free-solution capillary electrophoresis using phosphate buffer and acidic pH. Am. Lab. (Shelton, CT.) 22:60.
39. WERNER, W. E., WIKTOROWICZ, J. E., and KASARDA, D. D. 1994. Wheat varietal identification by capillary electrophoresis of gliadins and high molecular weight glutenin subunits. Cereal Chem. 71:397.