An in vitro injury model for SH-SY5Y neuroblastoma cells: Effect of strain and strain rate

Journal of Neuroscience Methods

Volumn 205, Issue 1, 2012, Pages 159-168

  Skotak, Maciej ^a   Wang, Fang ^a   Chandra, Namas ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

In vitro TBI model; Strain and strain rate effect; Stretch induced injury; Viability scale

Indexed keywords

ARTICLE; CELL MEMBRANE; CELL STRESS; CELL STRETCHING; CELL VIABILITY; CONTROLLED STUDY; DISEASE MODEL; HUMAN; HUMAN CELL; IN VITRO STUDY; NEUROBLASTOMA; PRIORITY JOURNAL; TENSILE STRENGTH; TRAUMATIC BRAIN INJURY;

ALGORITHMS; AXONS; BIOMECHANICS; BRAIN INJURIES; CALIBRATION; CELL DEATH; CELL LINE, TUMOR; CELL MEMBRANE PERMEABILITY; CELL SHAPE; CELL SURVIVAL; CYTOLOGICAL TECHNIQUES; DATA INTERPRETATION, STATISTICAL; DIMETHYLPOLYSILOXANES; ELASTICITY; HUMANS; LASER SCANNING CYTOMETRY; NEURONS; STRESS, MECHANICAL; TENSILE STRENGTH;

EID: 84862796113     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2012.01.001     Document Type: Article

References (39)

1. Arun P., Spadaro J., John J., Gharavi R.B., Bentley T.B., Nambiar M.P. Studies on blast traumatic brain injury using in vitro model with shock tube. Neuroreport 2011, 22:379-384.
2. Bayly P.V., Black E.E., Pedersen R.C., Leister E.P., Genin G.M. In vivo imaging of rapid deformation and strain in an animal model of traumatic brain injury. J Biomech 2006, 39:1086-1095.
3. Bose A., Mouton-Liger F., Paquet C., Mazot P., Vigny M., Gray F., et al. Modulation of tau phosphorylation by the kinase PKR: implications in Alzheimer's disease. Brain Pathol 2011, 21:189-200.
4. i in NG108-15 cells subjected to high strain rate deformation and chemical hypoxia: an in vitro model for neural trauma. J Neurotrauma 1996, 13:395-407.
5. Cargill R.S., Dee K.C., Malcolm S. An assessment of the strength of NG108-15 cell adhesion to chemically modified surfaces. Biomaterials 1999, 20:2417-2425.
6. Cater H.L., Sundstrom L.E., Morrison B.I. Temporal development of hippocampal cell death is dependent on tissue strain but not strain rate. J Biomech 2006, 39:2810-2818.
7. Cernak I., Noble-Haeusslein L.J. Traumatic brain injury: an overview of pathobiology with emphasis on military populations. J Cereb Blood Flow Metab 2010, 30:255-266.
8. Chen Y.C., Smith D.H., Meaney D.F. In vitro approaches for studying blast-induced traumatic brain injury. J Neurotrauma 2009, 26:861-876.
9. Cullen D.K., Simon C.M., LaPlaca M.C. Strain rate-dependent induction of reactive astrogliosis and cell death in three-dimensional neuronal-astrocytic co-cultures. Brain Res 2007, 1158:103-115.
10. Decressac M., Pain S., Chabeauti P.Y., Frangeul L., Thiriet N., Herzog H., et al. Neuroprotection by neuropeptide Y in cell and animal models of Parkinson's disease. Neurobiol Aging 2011.
11. Elkin B.S., Morrison B.I. Region-specific tolerance criteria for the living brain. Stapp Car Crash J 2007, 51:127-138.
12. Ellis E.F., McKinney J.S., Willoughby K.A., Liang S., Povlishock J.T. A new model for rapid stretch-induced injury of cells in culture: characterization of the model using astrocytes. J Neurotrauma 1995, 12:325-339.
13. Engel D.C., Slemmer J.E., Vlug A.S., Maas A.I., Weber J.T. Combined effects of mechanical and ischemic injury to cortical cells: secondary ischemia increases damage and decreases effects of neuroprotective agents. Neuropharmacology 2005, 49:985-995.
14. Geddes-Klein D.M., Schiffman K.B., Meaney D.F. Mechanisms and consequences of neuronal stretch injury in vitro differ with the model of trauma. J Neurotrauma 2006, 23:193-204.
15. Geddes D.M., Cargill R.S. An in vitro model of neural trauma: device characterization and calcium response to mechanical stretch. J Biomech Eng 2001, 123:247-255.
16. Geddes D.M., Cargill R.S., LaPlaca M.C. Mechanical stretch to neurons results in a strain rate and magnitude-dependent increase in plasma membrane permeability. J Neurotrauma 2003, 20:1039-1049.
17. Harnett M.M. Laser scanning cytometry: understanding the immune system in situ. Nat Rev Immunol 2007, 7:897-904.
18. LaPlaca M.C., Cullen D.K., McLoughlin J.J., Cargill R.S. High rate shear strain of three-dimensional neural cell cultures: a new in vitro traumatic brain injury model. J Biomech 2005, 38:1093-1105.
19. LaPlaca M.C., Lee V.M., Thibault L.E. An in vitro model of traumatic neuronal injury: loading rate-dependent changes in acute cytosolic calcium and lactate dehydrogenase release. J Neurotrauma 1997, 14:355-368.
20. LaPlaca M.C., Prado G.R. Neural mechanobiology and neuronal vulnerability to traumatic loading. J Biomech 2010, 43:71-78.
21. LaPlaca M.C., Thibault L.E. An in vitro traumatic injury model to examine the response of neurons to a hydrodynamically-induced deformation. Ann Biomed Eng 1997, 25:665-677.
22. Lusardi T.A., Rangan J., Sun D., Smith D.H., Meaney D.F. A device to study the initiation and propagation of calcium transients in cultured neurons after mechanical stretch. Ann Biomed Eng 2004, 32:1546-1558.
23. Lusardi T.A., Wolf J.A., Putt M.E., Smith D.H., Meaney D.F. Effect of acute calcium influx after mechanical stretch injury in vitro on the viability of hippocampal neurons. J Neurotrauma 2004, 21:61-72.
24. Morrison B.I., Cater H.L., Benham C.D., Sundstrom L.E. An in vitro model of traumatic brain injury utilising two-dimensional stretch of organotypic hippocampal slice cultures. J Neurosci Methods 2006, 150:192-201.
25. Morrison B.I., Elkin B.S., Dolle J.P., Yarmush M.L. In vitro models of traumatic brain injury. Annu Rev Biomed Eng 2011, 13:91-126.
26. Morrison B.I., Meaney D.F., Margulies S.S., McIntosh T.K. Dynamic mechanical stretch of organotypic brain slice cultures induces differential genomic expression: relationship to mechanical parameters. J Biomech Eng 2000, 122:224-230.
27. Morrison B.I., Meaney D.F., McIntosh T.K. Mechanical characterization of an in vitro device designed to quantitatively injure living brain tissue. Ann Biomed Eng 1998, 26:381-390.
28. Morrison B.I., Saatman K.E., Meaney D.F., McIntosh T.K. In vitro central nervous system models of mechanically induced trauma: a review. J Neurotrauma 1998, 15:911-928.
29. Nampiaparampil D.E. Prevalence of chronic pain after traumatic brain injury: a systematic review. JAMA 2008, 300:711-719.
30. Oliver J.D., Anderson S., Troy J.L., Brenner B.M., Deen W.H. Determination of glomerular size-selectivity in the normal rat with Ficoll. J Am Soc Nephrol 1992, 3:214-228.
31. Pfister B.J., Weihs T.P., Betenbaugh M., Bao G. An in vitro uniaxial stretch model for axonal injury. Ann Biomed Eng 2003, 31:589-598.
32. Pike B.R., Zhao X., Newcomb J.K., Glenn C.C., Anderson D.K., Hayes R.L. Stretch injury causes calpain and caspase-3 activation and necrotic and apoptotic cell death in septo-hippocampal cell cultures. J Neurotrauma 2000, 17:283-298.
33. Simon C.M., Sharif S., Tan R.P., LaPlaca M.C. Spinal cord contusion causes acute plasma membrane damage. J Neurotrauma 2009, 26:563-574.
34. Slemmer J.E., Matser E.J., De Zeeuw C.I., Weber J.T. Repeated mild injury causes cumulative damage to hippocampal cells. Brain 2002, 125:2699-2709.
35. Slemmer J.E., Weber J.T., De Zeeuw C.I. Cell death, glial protein alterations and elevated S-100 beta release in cerebellar cell cultures following mechanically induced trauma. Neurobiol Dis 2004, 15:563-572.
36. Smith D.H., Wolf J.A., Lusardi T.A., Lee V.M., Meaney D.F. High tolerance and delayed elastic response of cultured axons to dynamic stretch injury. J Neurosci 1999, 19:4263-4269.
37. Stroetz R.W., Vlahakis N.E., Walters B.J., Schroeder M.A., Hubmayr R.D. Validation of a new live cell strain system: characterization of plasma membrane stress failure. J Appl Physiol 2001, 90:2361-2370.
38. Winston F.K., Macarak E.J., Gorfien S.F., Thibault L.E. A system to reproduce and quantify the biomechanical environment of the cell. J Appl Physiol 1989, 67:397-405.
39. Zipfel G.J., Babcock D.J., Lee J.M., Choi D.W. Neuronal apoptosis after CNS injury: the roles of glutamate and calcium. J Neurotrauma 2000, 17:857-869.