Rapid detection and differentiation of Clonorchis sinensis and Opisthorchis viverrini eggs in human fecal samples using a duplex real-time fluorescence resonance energy transfer PCR and melting curve analysis

Parasitology Research

Volumn 111, Issue 1, 2012, Pages 89-96

  Sanpool, Oranuch ^a,b   Intapan, Pewpan M ^a,b   Thanchomnang, Tongjit ^b,c   Janwan, Penchom ^a,b   Lulitanond, Viraphong ^a,b   Doanh, Pham Ngoc ^d   Van Hien, Hoang ^d   Dung, Do Trung ^e   Maleewong, Wanchai ^a,b   Nawa, Yukifumi ^a  

^a KHON KAEN UNIVERSITY   (Thailand)

^b KHON KAEN UNIVERSITY   (Thailand)

^c MAHASARAKHAM UNIVERSITY   (Thailand)

^d INSTITUTE OF ECOLOGY AND BIOLOGICAL RESOURCES   (Viet Nam)

^e National Institute of Malariology Parasitology and Entomology Hanoi   (Viet Nam)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE SUBUNIT 2; UNCLASSIFIED DRUG;

AMPLICON; ARTICLE; CLONORCHIASIS; CLONORCHIS SINENSIS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIFFERENTIAL DIAGNOSIS; DNA DETERMINATION; DNA EXTRACTION; DUPLEX REAL TIME FLUORESCENCE RESONANCE ENERGY TRANSFER POLYMERASE CHAIN REACTION; FECES ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH RESOLUTION MELTING ANALYSIS; HUMAN; LIMIT OF DETECTION; NONHUMAN; NUCLEIC ACID AMPLIFICATION; NUCLEOTIDE SEQUENCE; OPISTHORCHIASIS; OPISTHORCHIS VIVERRINI; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; SPECIES DIFFERENTIATION; TEMPERATURE;

ADULT; ANIMALS; CLONORCHIASIS; CLONORCHIS SINENSIS; DNA PRIMERS; FECES; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; NADH DEHYDROGENASE; OPISTHORCHIASIS; OPISTHORCHIS; PARASITOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRANSITION TEMPERATURE;

CLONORCHIS SINENSIS; FASCIOLA HEPATICA; OPISTHORCHIS VIVERRINI;

EID: 84862772882     PISSN: 09320113     EISSN: 14321955     Source Type: Journal    
DOI: 10.1007/s00436-011-2804-7     Document Type: Article

References (43)

1. XQ Cai HQ Yu JS Bai JD Tang XC Hu DH Chen RL Zhang MX Chen L Ai XQ Zhu 2012 Development of a TaqMan based real-time PCR assay for detection of Clonorchis sinensis DNA in human stool samples and fishes Parasitol Int 61 183 186 21729765 10.1016/j.parint.2011.06.010 1:CAS:528:DC%2BC3MXhsFygtb%2FF
2. Y Caron D Rondelaud B Losson 2008 The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp Parasitol Res 103 735 744 18574594 10.1007/s00436-008-1086-1
3. L Dormond K Jaton-Ogay S de Vallière B Genton J Bille G Greub 2011 Multiplex real-time PCR for the diagnosis of malaria: correlation with microscopy Clin Microbiol Infect 17 469 475 20298268 10.1111/j.1469-0691.2010. 03218.x 1:STN:280:DC%2BC3M7ot1antg%3D%3D
4. P Dorny N Praet N Deckers S Gabriel 2009 Emerging food-borne parasites Vet Parasitol 163 196 206 19559535 10.1016/j.vetpar.2009.05.026 1:STN:280:DC%2BD1MvntVKnuw%3D%3D
5. DB Elkins M Haswell-Elkins RM Anderson 1986 The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection Trans R Soc Trop Med Hyg 80 774 792 3603617 10.1016/0035-9203(86) 90384-6 1:STN:280:DyaL2s3msVKmug%3D%3D
6. B Fried A Abruzzi 2010 Food-borne trematode infections of humans in the United States of America Parasitol Res 106 1263 1280 20352454 10.1007/s00436-010-1807-0
7. B Fried A Reddy D Mayer 2011 Helminths in human carcinogenesis Cancer Lett 305 239 249 20667649 10.1016/j.canlet.2010.07.008 1:CAS:528: DC%2BC3MXlsV2gs7o%3D
8. RS Galen 1980 Predictive value and efficiency of laboratory testing Pediatr Clin North Am 27 861 869 7454413 1:STN:280:DyaL3M7gtVagug%3D%3D
9. ST Hong Y Fang 2012 Clonorchis sinensis and clonorchiasis, an update Parasitol Int 61 17 24 21741496 10.1016/j.parint.2011.06.007 1:CAS:528:DC%2BC3MXhsFygtb%2FL
10. SY Hwang SH Kim GY Lee VT Hang CS Moon JH Shin WL Koo SY Kim HJ Park HO Park WG Kho 2011 A novel real-time PCR assay for the detection of Plasmodium falciparum and Plasmodium vivax malaria in low parasitized individuals Acta Trop 120 40 45 21664340 10.1016/j.actatropica.2011.05.006 1:CAS:528: DC%2BC3MXhtFShurrJ
11. PM Intapan T Thanchomnang V Lulitanond P Pongsaskulchoti W Maleewong 2008 Detection of Opisthorchis viverrini in infected bithynid snails by real-time fluorescence resonance energy transfer PCR-based method and melting curve analysis Parasitol Res 103 649 655 18512076 10.1007/s00436-008-1026-0
12. PM Intapan T Thanchomnang V Lulitanond P Phongsaskulchoti W Maleewong 2008 Real-time fluorescence resonance energy transfer PCR with melting curve analysis for the detection of Opisthorchis viverrini in fish intermediate hosts Vet Parasitol 157 65 71 18760538 10.1016/j.vetpar.2008.07.012 1:CAS:528:DC%2BD1cXhtFartLrO
13. PM Intapan T Thanchomnang V Lulitanond P Pongsaskulchoti W Maleewong 2009 Rapid molecular detection of Opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction AmJTrop Med Hyg 81 917 920 10.4269/ajtmh.2009.09-0275 1:CAS:528:DC%2BD1MXhsFejsLzE
14. PM Intapan T Thanchomnang V Lulitanond W Maleewong 2009 Rapid detection of Wuchereria bancrofti and Brugia malayi in mosquito vectors (Diptera: Culicidae) using a real-time fluorescence resonance energy transfer multiplex PCR and melting curve analysis J Med Entomol 46 158 164 19198529 10.1603/033.046.0119 1:CAS:528:DC%2BD1MXhvV2ltL4%3D
15. P Janwan PM Intapan T Thanchomnang V Lulitanond W Anamnart W Maleewong 2011 Rapid detection of Opisthorchis viverrini and Strongyloides stercoralis in human fecal samples using a duplex real-time PCR and melting curve analysis Parasitol Res 109 1593 1601 21537984 10.1007/s00436-011-2419-z
16. S Kang T Sultana VB Loktev S Wongratanacheewin WM Sohn KS Eom JK Park 2008 Molecular identification and phylogenetic analysis of nuclear rDNA sequences among three opisthorchid liver fluke species (Opisthorchiidae: Trematoda) Parasitol Int 57 191 197 18276183 10.1016/j.parint.2007.12.007 1:CAS:528:DC%2BD1cXjs1WjsL8%3D
17. J Keiser J Utzinger 2007 Food-borne trematodiasis: current chemotherapy and advances with artemisinins and synthetic trioxolanes Trends Parasitol 23 555 562 17950667 10.1016/j.pt.2007.07.012 1:CAS:528:DC%2BD2sXht1Kgtr%2FL
18. J Keiser J Utzinger 2009 Food-borne trematodiases Clin Microbiol Rev 22 466 483 19597009 10.1128/CMR.00012-09
19. K Khairnar D Martin R Lau F Ralevski IDR Pilla 2009 Multiplex real-time quantitative PCR, microscopy and rapid diagnostic immuno-chromatographic tests for the detection of Plasmodium spp performance, limit of detection analysis and quality assurance Malar J 8 284 20003199 10.1186/1475-2875-8-284
20. EM Kim JJ Verweij A Jalili L van Lieshout MH Choi YM Bae MK Lim ST Hong 2009 Detection of Clonorchis sinensis in stool samples using real-time PCR Ann Trop Med Parasitol 103 513 518 19695156 10.1179/136485909X451834 1:CAS:528:DC%2BD1MXhtF2qs73E
21. TH Le N Van De D Blair P Sithithaworn DP McManus 2006 Clonorchis sinensis and Opisthorchis viverrini: development of a mitochondrial-based multiplex PCR for their identification and discrimination Exp Parasitol 112 109 114 16310774 10.1016/j.exppara.2005.09.012 1:CAS:528:DC%2BD28XjvVCmtA%3D%3D
22. E Lyon CT Wittwer 2009 LightCycler technology in molecular diagnostics J Mol Diagn 11 93 101 19196999 10.2353/jmoldx.2009.080094 1:CAS:528: DC%2BD1MXjs12qs70%3D
23. M Montes C Sawhney N Barros 2010 Strongyloides stercoralis: there but not seen Curr Opin Infect Dis 23 500 504 20733481 10.1097/QCO.0b013e32833df718
24. GM Park 2007 Genetic comparison of liver flukes, Clonorchis sinensis and Opisthorchis viverrini, based on rDNA and mtDNA gene sequences Parasitol Res 100 351 357 16902795 10.1007/s00436-006-0269-x
25. SM Rahman YM Bae ST Hong MH Choi 2011 Early detection and estimation of infection burden by real-time PCR in rats experimentally infected with Clonorchis sinensis Parasitol Res 109 297 303 21279385 10.1007/s00436-011-2253-3
26. M Sato U Thaenkham P Dekumyoy J Waikagul 2009 Discrimination of O. viverrini, C. sinensis, H. pumilio and H. taichui using nuclear DNA-based PCR targeting ribosomal DNA ITS regions Acta Trop 109 81 83 18952037 10.1016/j.actatropica.2008.09.015 1:CAS:528:DC%2BD1cXhsVertb%2FP
27. SV Shekhovtsov AV Katokhin KV Romanov VV Besprozvannykh KP Fedorov NI Yurlova EA Serbina P Sithithaworn NA Kolchanov VA Mordvinov 2009 A novel nuclear marker, Pm-int9, for phylogenetic studies of Opisthorchis felineus, Opisthorchis viverrini, and Clonorchis sinensis (Opisthorchiidae, Trematoda) Parasitol Res 106 293 297 19777262 10.1007/s00436-009-1628-1
28. P Sri-Aroon PM Intapan C Lohachit P Phongsasakulchoti T Thanchomnang V Lulitanond A Hiscox S Phompida P Sananikhom W Maleewong PT Brey 2011 Molecular evidence of Opisthorchis viverrini in infected bithyniid snails in the Lao People's Democratic Republic by specific hybridization probe-based real-time fluorescence resonance energy transfer PCR method Parasitol Res 108 973 978 21069540 10.1007/s00436-010-2140-3
29. B Sripa S Kaewkes PM Intapan W Maleewong PJ Brindley 2010 Food-borne trematodiases in Southeast Asia epidemiology, pathology, clinical manifestation and control Adv Parasitol 72 305 350 20624536 10.1016/S0065-308X(10)72011-X
30. WM Stauffer JS Sellman PF Walker 2004 Biliary liver flukes (opisthorchiasis and clonorchiasis) in immigrants in the United States: often subtle and diagnosed years after arrival J Travel Med 11 157 159 15710057
31. N Suksumek K Leelawat S Leelawat B Russell U Lek-Uthai 2008 TaqMan real-time PCR assay for specific detection of Opisthorchis viverrini DNA in Thai patients with hepatocellular carcinoma and cholangiocarcinoma Exp Parasitol 119 217 224 18329641 10.1016/j.exppara.2008.01.018 1:CAS:528:DC%2BD1cXlsF2qur8%3D
32. J Sun J Xu P Liang Q Mao Y Huang X Lv C Deng C Liang GS de Hoog X Yu 2011 Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA) Parasit Vectors 4 98 21649899 10.1186/1756-3305-4-98 1:CAS:528:DC%2BC3MXnslCns7k%3D
33. M Taniuchi JJ Verweij Z Noor SU Sobuz L Lieshout WA Petri Jr R Haque ER Houpt 2011 High throughput multiplex PCR and probe-based detection with Luminex beads for seven intestinal parasites AmJTrop Med Hyg 84 332 337 10.4269/ajtmh.2011.10-0461 1:CAS:528:DC%2BC3MXisF2ntr4%3D
34. R ten Hove T Schuurman M Kooistra L Möller L van Lieshout JJ Verweij 2007 Detection of diarrhoea-causing protozoa in general practice patients in the Netherlands by multiplex real-time PCR Clin Microbiol Infect 13 1001 1007 17714523 10.1111/j.1469-0691.2007.01788.x
35. RJ ten Hove JJ Verweij K Vereecken K Polman L Dieye L van Lieshout 2008 Multiplex real-time PCR for the detection and quantification of Schistosoma mansoni and S. haematobium infection in stool samples collected in northern Senegal Trans R Soc Trop Med Hyg 102 179 185 18177680 10.1016/j.trstmh.2007.10. 011
36. RJ ten Hove M van Esbroeck T Vervoort J van den Ende L van Lieshout JJ Verwei 2009 Molecular diagnostics of intestinal parasites in returning travellers Eur J Clin Microbiol Infect Dis 28 1045 1053 19415354 10.1007/s10096-009-0745-1
37. RJ Traub J Macaranas M Mungthin S Leelayoova T Cribb KD Murrell RC Thompson 2009 A new PCR-based approach indicates the range of Clonorchis sinensis now extends to Central Thailand PLoS Negl Trop Dis 3 367 10.1371/journal.pntd.0000367
38. L van Lieshout JJ Verweij 2010 Newer diagnostic approaches to intestinal protozoa Curr Opin Infect Dis 23 488 493 20683263 10.1097/QCO.0b013e32833de0eb
39. V Veron S Simon B Carme 2009 Multiplex real-time PCR detection of P. falciparum, P. vivax and P. malariae in human blood samples Exp Parasitol 121 346 351 19124021 10.1016/j.exppara.2008.12.012 1:CAS:528:DC%2BD1MXisFKrsLY%3D
40. JJ Verweij EA Brienen J Ziem L Yelifari AM Polderman L Van Lieshout 2007 Simultaneous detection and quantification of Ancylostoma duodenale, Necator americanus, and Oesophagostomum bifurcum in fecal samples using multiplex real-time PCR AmJTrop Med Hyg 77 685 690 1:CAS:528:DC%2BD2sXht12iurrL
41. World Health Organization 1995 Control of foodborne trematode infections. Report of a WHO Study Group World Health Organ Tech Rep Ser 849 1 157
42. O Yossepowitch T Gotesman M Assous E Marva R Zimlichman M Dan 2004 Opisthorchiasis from imported raw fish Emerg Infect Dis 10 2122 2126 15663848 10.3201/eid1012.040410
43. DS Zarlenga J Higgins 2001 PCR as a diagnostic and quantitative technique in veterinary parasitology Vet Parasitol 101 215 230 11707298 10.1016/S0304-4017(01)00568-4 1:CAS:528:DC%2BD3MXot1OgsL8%3D