A novel real-time PCR assay for the detection of Plasmodium falciparum and Plasmodium vivax malaria in low parasitized individuals

Acta Tropica

Volumn 120, Issue 1-2, 2011, Pages 40-45

  Hwang, Seung Young ^a   Kim, So Hee ^a,b   Lee, Ga Young ^a   Hang, Vu Thi Thu ^a,b   Moon, Chi Sook ^c   Shin, Jeong Hwan ^a   Koo, Wan Lim ^d   Kim, Seong Youl ^d   Park, Hae Joon ^d   Park, Han Oh ^d   Kho, Weon Gyu ^a,b  

^a Inje University College of Medicine   (South Korea)

^b Inje University   (South Korea)

^c Inje University College of Medicine   (South Korea)

^d Bioneer Corporation   (South Korea)

Author keywords

Diagnosis; Low parasitemia; Malaria; Real time PCR

Indexed keywords

MALARIA; MOLECULAR ANALYSIS; PARASITE; POLYMERASE CHAIN REACTION;

ARTICLE; BLOOD SAMPLING; BLOOD SMEAR; CONTROLLED STUDY; FREEZE DRYING; GIEMSA STAIN; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALARIA CONTROL; MALARIA FALCIPARUM; MOLECULAR DIAGNOSIS; MULTIPLEX POLYMERASE CHAIN REACTION; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; PARASITEMIA; PLASMODIUM FALCIPARUM; PLASMODIUM VIVAX; PLASMODIUM VIVAX MALARIA; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; CLINICAL LABORATORY TECHNIQUES; DNA, PROTOZOAN; HUMANS; MALARIA; MICROSCOPY; MULTIPLEX POLYMERASE CHAIN REACTION; PARASITEMIA; PLASMODIUM FALCIPARUM; PLASMODIUM VIVAX; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY;

PLASMODIUM FALCIPARUM; PLASMODIUM VIVAX;

EID: 80052098612     PISSN: 0001706X     EISSN: 18736254     Source Type: Journal    
DOI: 10.1016/j.actatropica.2011.05.006     Document Type: Article

References (20)

1. Amexo M., Tolhurst R., Barnish G., Bates I. Malaria misdiagnosis: effects on the poor and vulnerable. Lancet 2004, 364:1896-1898.
2. Berry A., Fabre R., Benoit-Vical F., Cassaing S., Magnaval J.F. Contribution of PCR-based methods to diagnosis and management of imported malaria. Med. Trop. (Mars). 2005, 65:176-183.
3. Boonma P., Christensen P., Suwanarusk R., Price R., Russell B., Lek-Uthai U. Comparison of three molecular methods for the detection and speciation of Plasmodium vivax and Plasmodium falciparum. Malar. J. 2007, 6(1):124-130.
4. Elsayed S., Plewes K., Church D., Chow B., Zhang K. Use of molecular beacon probes for real-time PCR detection of Plasmodium falciparum and other plasmodium species in peripheral blood specimens. J. Clin. Microbiol. 2006, 44:622-624.
5. Farcas G.A., Zhong K.J.Y., Mazzulli T., Kain K.C. Evaluation of the RealArt malaria LC real-time PCR assay for malaria diagnosis. J. Clin. Microbiol. 2004, 42:636-638.
6. Fryer J.F., Minor P.D Standardisation of nucleic acid amplification assays used in clinical diagnostics: a report of the first meeting of the SoGAT clinical diagnostics working group. J. Clin. Virol. 2009, 44:103-105.
7. Gama B.E., Silva-Pires Fdo E., Lopes M.N., Cardoso M.A., Britto C., Torres K.L., de Mendonça Lima L., de Souza J.M., Daniel-Ribeiro C.T., Ferreira-da-Cruz Mde F. Real-time PCR versus conventional PCR for malaria parasite detection in low-grade parasitemia. Exp. Parasitol. 2007, 116:427-432.
8. Hänscheid T., Grobusch M.P. How useful is PCR in the diagnosis of malaria?. Trends Parasitol. 2002, 18(9):395-398.
9. Igbinosa O., Igbinosa O., Asowata O., Jeffery C. A sequential review on accuracy of detecting malaria parasitemia in developing countries with large restriction on resources. J. Med. Med. Sci. 2010, 1(9):385-390.
10. Kain K.C., Brown A.E., Mirabelli L., Webster H.K. Detection of Plasmodium vivax by polymerase chain reaction in a field study. J. Infect. Dis. 1993, 168(5):1323-1326.
11. Kho W.G., Chung J.Y., Sim E.J., Kim M.Y., Kim D.W., Jongwutiwes S., Tanabe K. A multiplex polymerase chain reaction for a differential diagnosis of Plasmodium falciparum and Plasmodium vivax. Parasitol. Int. 2003, 52:229-236.
12. Mangold K.A., Manson R.U., Koay E.S., Stephens L., Regner M., Thomson R.B., Peterson L.R., Kaul K.L. Real-time PCR for detection and identification of Plasmodium spp. J. Clin. Microbiol. 2005, 43:2435-2440.
13. Padley D.J., Heath A.B., Sutherland C., Chiodini P.L., Baylis S.A. Establishment of the 1st World Health Organization International Standard for Plasmodium falciparum DNA for nucleic acid amplification technique (NAT)-based assays. Malar. J. 2008, 7:139.
14. Perandin F., Manca N., Calderaro A., Piccolo G., Galati L., Ricci L., Medici M.C., Arcangeletti M.C., Snounou G., Dettori G., Chezzi C. Development of a real-time PCR assay for detection of Plasmodium falciparum Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosis. J. Clin. Microbiol. 2004, 42:1214-1219.
15. Snounou G., Viriyakosol S., Zhu X.P., Jarra W., Pinheiro L., do Rosario V.E., Thaithong S., Brown K.N. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol. Biochem. Parasitol. 1993, 61:315-320.
16. Tham J.M., Lee S.H., Tan T.M., Ting R.C., Kara U.A. Detection and species determination of malaria parasites by PCR: comparison with microscopy and with ParaSight-F and ICT malaria Pf tests in a clinical environment. J. Clin. Microbiol. 1999, 37:1269-1273.
17. Vo T.K., Bigot P., Gazin P., Sinou V., De Pina J.J., Huynh D.C., Fumoux F., Parzy D. Evaluation of a real-time PCR assay for malaria diagnosis in patients from Vietnam and in returned travellers. Trans. R. Soc. Trop. Med. Hyg. 2007, 101:422-428.
18. Wilcox A. A Manual for the Microscopical Diagnosis of Malaria in Humans 1960, Department of Health, Education, and Welfare, Washington, DC, USA.
19. World Health Organization, 2004. Recommendations for the preparation, characterization and establishment of international and other biological reference materials. WHO/BS/04.119 (revised 2004).
20. World Health Organization, 2009. World Malaria Report 2009. Impact of malaria control. Geneva. Available: /. http://whqlibdoc.who.int/publications/2009/9789241563901_eng.PDF.