Discrimination of O. viverrini, C. sinensis, H. pumilio and H. taichui using nuclear DNA-based PCR targeting ribosomal DNA ITS regions

Acta Tropica

Volumn 109, Issue 1, 2009, Pages 81-83

  Sato, Megumi ^a   Thaenkham, Urusa ^a   Dekumyoy, Paron ^a   Waikagul, Jitra ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Copro DNA diagnosis; Minute intestinal flukes; Opisthorchis viverrini; PCR; Ribosomal DNA ITS regions

Indexed keywords

CELL NUCLEUS DNA; RIBOSOME DNA;

DNA; FECES; FLATWORM; MORPHOLOGY; POLYMERASE CHAIN REACTION;

AMPLICON; ANIMAL CELL; ARTICLE; CLONORCHIS SINENSIS; DNA DETERMINATION; DNA EXTRACTION; FASCIOLIASIS; FECES ANALYSIS; HAPLORCHIS; HAPLORCHIS PUMILIO; HAPLORCHIS TAICHUI; NONHUMAN; NUCLEOTIDE SEQUENCE; OPISTHORCHIASIS; OPISTHORCHIS VIVERRINI; POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SMEAR; SPECIES DIFFERENCE; SPECIES DIFFERENTIATION; TREMATODE;

ANIMALS; CLONORCHIS SINENSIS; DNA, HELMINTH; DNA, RIBOSOMAL; DNA, RIBOSOMAL SPACER; HETEROPHYIDAE; OPISTHORCHIS; POLYMERASE CHAIN REACTION; SPECIES SPECIFICITY;

CLONORCHIS SINENSIS; HAPLORCHIS PUMILIO; HAPLORCHIS TAICHUI; OPISTHORCHIS VIVERRINI; TREMATODA;

EID: 57049083447     PISSN: 0001706X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actatropica.2008.09.015     Document Type: Article

References (11)

1. Chai J.Y., Park J.H., Han E.T., Guk S.M., Shin E.H., Lin A., Kim J.L., Sohn W.M., Yong T.S., Eom K.S., Min D.Y., Hwang E.H., Phommmasack B., Insisiengmay B., and Rim H.J. Mixed infections with Opisthorchis viverrini and intestinal flukes in residents of Vientiane Municipality and Saravane Province in Laos. J. Helminthol. 79 (2005) 283-289
2. Duenngai K., Sithithaworn P., Rudrappa U.K., Iddya K., Laha T., Stensvold R., Standgaard H., and Johansen M.V. Improvement of polymerase chain reaction for detection 1 of Opisthorchis viverrini DNA in human stool samples. J. Clin. Microbiol. 46 (2008) 366-368
3. Kaewkes S., Elkins D.B., Sithithaworn P., and Haswellelkins M.R. Comparative studies on the morphology of the eggs of Opisthorchis viverrini and Lecithodendriid Trematodes. Southeast Asian J. Trop. Med. Public Health 22 (1991) 623-630
4. Le T.H., De N.V., Blair D., Sithithaworn P., and McManus D.P. Clonorchis sinensis and Opisthorchis viverrini: development of a mitochondrial-based multiplex PCR for their identification and discrimination. Exp. Parasitol. 112 (2006) 109-114
5. Parvathi A., Kumar H.S., Prakasha B.K., Lu J., Xu X., Hu W., Feng Z., Karunasagar I., and Karunasagar I. Clonorchis sinensis: development and evaluation of a nested polymerase chain reaction (PCR) assay. Exp. Parasitol. 115 (2007) 291-295
6. Stensvold C.R., Saijuntha W., Sithithaworn P., Wongratanacheewin S., Strandgaard H., Ornbjerg N., and Johansen M.V. Evaluation of PCR based coprodiagnosis of human opisthorchiasis. Acta Trop. 97 (2006) 26-30
7. Thaenkham U., Visetsuk K., Dung D.T., and Waikagul J. Discrimination of Opisthorchis viverrini from Haplorchis taichui using COI sequence marker. Acta Trop. 103 (2007) 26-32
8. Waikagul J., Anantaphruti M.T., Nuamtanong S., and Sanguankait S. Evaluation of the modified formalin detergent technique for detection of intestinal parasites. Collected Papers on the Control of Soil-Transmitted Helminthiases vol. 6 (1997) pp. 5-11
9. Waikagul J., and Radomyos P. Intestinal trematode infections in Thailand. Asian Parasitology vol. 1 (2005), Food-Borne Helminthiasis in Asia, FAP Journal Ltd., Chiba pp. 103-112
10. Wongratanacheewin S., Pumidonming W., Sermswan R.W., and Maleewong W. Development of a PCR-based method for the detection of Opisthorchis viverrini in experimentally infected hamsters. Parasitology 122 (2001) 175-180
11. Wongratanacheewin S., Pumidonming W., Sermswan R.W., Pipitgool V., and Maleewong W. Detection of Opisthorchis viverrini in human stool specimens by PCR. J. Clin. Microbiol. 40 (2002) 3879-3880