A new electrochemical assay method for gene expression using hela cells with a secreted alkaline phosphatase (SEAP) reporter system

Biotechnology and Bioengineering

Volumn 109, Issue 8, 2012, Pages 2163-2167

  Şen, Mustafa ^a   Ino, Kosuke ^a   Shiku, Hitoshi ^a   Matsue, Tomokazu ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

Biosensor; Cell array; Electrochemistry; Lab on a chip; Reporter gene assay

Indexed keywords

CELL ARRAY; CELL-BASED; ELECTROCHEMICAL ASSAYS; HELA CELL; INTACT CELLS; LAB ON A CHIP; MICROWELLS; PHYSIOLOGICAL CONDITION; REPORTER GENE ASSAYS; REPORTER SYSTEMS; SCANNING ELECTROCHEMICAL MICROSCOPY; SECRETED ALKALINE PHOSPHATASE; SINGLE-CELL LEVEL; TRANSFECTED CELLS;

ANALYTIC EQUIPMENT; BIOSENSORS; CELL CULTURE; ELECTROCHEMISTRY; GENE EXPRESSION; PHOSPHATASES; SCANNING ELECTRON MICROSCOPY; SCANNING PROBE MICROSCOPY;

BIOASSAY;

ALKALINE PHOSPHATASE;

ACCURACY; ARTICLE; BIOSENSOR; DEVICES; ELECTROCHEMICAL DETECTION; ENZYME RELEASE; GENE EXPRESSION; HUMAN; HUMAN CELL; PROTEIN EXPRESSION; PROTEIN MICROARRAY; SCANNING ELECTROCHEMICAL MICROSCOPY;

ALKALINE PHOSPHATASE; ELECTROCHEMICAL TECHNIQUES; GENE EXPRESSION PROFILING; GENES, REPORTER; HELA CELLS; HUMANS; MICROARRAY ANALYSIS; TRANSFECTION;

EID: 84862699254     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24461     Document Type: Article

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