Determination and control of low-level amino acid misincorporation in human thioredoxin protein produced in a recombinant Escherichia coli production system

Biotechnology and Bioengineering

Volumn 109, Issue 8, 2012, Pages 1987-1995

  Harris, Robert P ^a   Mattocks, Jo ^a   Green, Philip S ^a   Moffatt, Frank ^a   Kilby, Peter M ^a  

^a JEALOTT S HILL INTERNATIONAL RESEARCH CENTRE   (United Kingdom)

Author keywords

Amino acid misincorporation; Amino acid substitution; Biopharmaceutical; HTRX; Protein mistranslation; TRX

Indexed keywords

ACTIVE MOLECULES; AMINO ACID COMPOSITIONS; AMINO ACID SUBSTITUTION; BIOPHARMACEUTICAL; CLINICAL APPLICATION; CURRENT TECHNOLOGY; E. COLI; ENDOGENOUS THIOREDOXIN; FERMENTATION MEDIA; GENE SEQUENCES; HEALTH IMPLICATIONS; HTRX; MASS SPECTROMETRY DATA; OVER-EXPRESSION; PRODUCTION PROCESS; PRODUCTION SYSTEM; PROTEOMIC TECHNOLOGIES; PROTEOMICS; RECOMBINANT ESCHERICHIA COLI; SCOPING; THERAPEUTIC APPLICATION; THIOREDOXINS; TRX;

AMINO ACIDS; ANTIBODIES; ESCHERICHIA COLI; MACHINERY; MASS SPECTROMETRY; MOLECULAR BIOLOGY; MOLECULES; PRODUCTION ENGINEERING;

RECOMBINANT PROTEINS;

AMINO ACID; ANTIBODY; RECOMBINANT PROTEIN; THIOREDOXIN;

AMINO ACID ANALYSIS; AMINO ACID COMPOSITION; AMINO ACID MISINCORPORATION; AMINO ACID SUBSTITUTION; ARTICLE; AUTOIMMUNITY; BIOPROCESS; BIOREACTOR; CAPILLARY CHROMATOGRAPHY; CONTROLLED STUDY; CROSS REACTION; DOWNSTREAM PROCESSING; ELECTROSPRAY MASS SPECTROMETRY; ESCHERICHIA COLI; EXPERIMENTAL TEST; FED BATCH FERMENTATION; FERMENTATION MEDIUM; GENE EXPRESSION SYSTEM; GENE SEQUENCE; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRACTICE GUIDELINE; PROTEIN ISOLATION; PROTEOMICS; RANDOM ERROR; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SCREENING; THEORETICAL SAMPLE;

AMINO ACIDS; BIOLOGICAL AGENTS; BIOTECHNOLOGY; ESCHERICHIA COLI; HUMANS; MASS SPECTROMETRY; RECOMBINANT PROTEINS; TECHNOLOGY, PHARMACEUTICAL; THIOREDOXINS;

ESCHERICHIA COLI;

EID: 84862667471     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24462     Document Type: Article

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