Cardiac transcription factor Nkx2.5 is downregulated under excessive O-GlcNAcylation condition

PLoS ONE

Volumn 7, Issue 6, 2012, Pages

  Kim, Hoe Suk ^a,b   Woo, Ji Soo ^a,b   Joo, Hyun Jung ^a,b   Moon, Woo Kyung ^a,b  

^a Seoul National University   (South Korea)

^b Seoul National University Hospital   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME INHIBITOR; N ACETYLGLUCOSAMINE; O LINKED N ACETYLGLUCOSAMINE; O LINKED N ACETYLGLUCOSAMINE TRANSFERASE; STREPTOZOCIN; TRANSCRIPTION FACTOR NKX2.5; TRANSFERASE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL STRAIN HEK293; CONTROLLED STUDY; DIABETIC CARDIOMYOPATHY; DOWN REGULATION; EMBRYO; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MALE; MOUSE; NONHUMAN; PROTEIN GLYCOSYLATION; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; STREPTOZOCIN DIABETES; TISSUE LEVEL;

ACETYLGLUCOSAMINE; ACYLATION; ANIMALS; DIABETES MELLITUS, EXPERIMENTAL; DOWN-REGULATION; HOMEODOMAIN PROTEINS; MICE; STREPTOZOCIN; TRANSCRIPTION FACTORS;

EID: 84862506186     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0038053     Document Type: Article

References (23)

1. Hunter T, Karin M, (1992) The regulation of transcription by phosphorylation. Cell 70: 375-387.
2. Hart GW, Housley MP, Slawson C, (2007) Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins. Nature 446: 1017-1022.
3. Hart GW, Kreppel LK, Comer FI, Arnold CS, Snow DM, et al. (1996) O-GlcNAcylation of key nuclear and cytoskeletal proteins: reciprocity with O-phosphorylation and putative roles in protein multimerization. Glycobiology 6: 711-716.
4. Kim HS, Kim EM, Lee J, Yang WH, Park TY, et al. (2006) Heat shock protein 60 modified with O-linked N-acetylglucosamine is involved in pancreatic beta-cell death under hyperglycemic conditions. FEBS Lett 580: 2311-2316.
5. Yang WH, Kim JE, Nam HW, Ju JW, Kim HS, et al. (2006) Modification of p53 with O-linked N-acetylglucosamine regulates p53 activity and stability. Nat Cell Biol 8: 1074-1083.
6. Zhang F, Su K, Yang X, Bowe DB, Paterson AJ, et al. (2003) O-GlcNAc modification is an endogenous inhibitor of the proteasome. Cell 115: 715-725.
7. Guinez C, Mir AM, Dehennaut V, Cacan R, Harduin-Lepers A, et al. (2008) Protein ubiquitination is modulated by O-GlcNAc glycosylation. FASEB J 22: 2901-2911.
8. Brownlee M, (2001) Biochemistry and molecular cell biology of diabetic complications. Nature 414: 813-820.
9. Clark RJ, McDonough PM, Swanson E, Trost SU, Suzuki M, et al. (2003) Diabetes and the accompanying hyperglycemia impairs cardiomyocyte calcium cycling through increased nuclear O-GlcNAcylation. J Biol Chem 278: 44230-44237.
10. Hu Y, Belke D, Suarez J, Swanson E, Clark R, et al. (2005) Adenovirus-mediated overexpression of O-GlcNAcase improves contractile function in the diabetic heart. Circ Res 96: 1006-1013.
11. Fiordaliso F, Leri A, Cesselli D, Limana F, Safai B, et al. (2001) Hyperglycemia activates p53 and p53-regulated genes leading to myocyte cell death. Diabetes 50: 2363-2375.
12. Lints TJ, Parsons LM, Hartley L, Lyons I, Harvey RP, (1993) Nkx-2.5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants. Development 119: 419-431.
13. Komuro I, Izumo S, (1993) Csx: a murine homeobox-containing gene specifically expressed in the developing heart. Proc Natl Acad Sci U S A 90: 8145-8149.
14. Hidaka K, Lee JK, Kim HS, Ihm CH, Iio A, et al. (2003) Chamber-specific differentiation of Nkx2.5-positive cardiac precursor cells from murine embryonic stem cells. FASEB J 17: 740-742.
15. Kasahara H, Lee B, Schott JJ, Benson DW, Seidman JG, et al. (2000) Loss of function and inhibitory effects of human CSX/NKX2.5 homeoprotein mutations associated with congenital heart disease. J Clin Invest 106: 299-308.
16. Akazawa H, Komuro I, (2005) Cardiac transcription factor Csx/Nkx2-5: Its role in cardiac development and diseases. Pharmacol Ther 107: 252-268.
17. Kim HS, Park SY, Choi YR, Kang JG, Joo HJ, et al. (2009) Excessive O-GlcNAcylation of proteins suppresses spontaneous cardiogenesis in ES cells. FEBS Lett 583: 2474-2478.
18. Kasahara H, Izumo S, (1999) Identification of the in vivo casein kinase II phosphorylation site within the homeodomain of the cardiac tisue-specifying homeobox gene product Csx/Nkx2.5. Mol Cell Biol 19: 526-536.
19. Wang J, Zhang H, Iyer D, Feng XH, Schwartz RJ, (2008) Regulation of cardiac specific nkx2.5 gene activity by small ubiquitin-like modifier. J Biol Chem 283: 23235-23243.
20. McNulty PH, (2007) Hexosamine biosynthetic pathway flux and cardiomyopathy in type 2 diabetes mellitus. Focus on "Impact of type 2 diabetes and aging on cardiomyocyte function and O-linked N-acetylglucosamine levels in the heart". Am J Physiol Cell Physiol 292: C1243-1244.
21. Ramirez-Correa GA, Jin W, Wang Z, Zhong X, Gao WD, (2008) O-linked GlcNAc modification of cardiac myofilament proteins: a novel regulator of myocardial contractile function. Circ Res 103: 1354-1358.
22. Kohda Y, Kanematsu M, Kono T, Terasaki F, Tanaka T, (2009) Protein O-glycosylation induces collagen expression and contributes to diabetic cardiomyopathy in rat cardiac fibroblasts. J Pharmacol Sci 111: 446-450.
23. Toko H, Zhu W, Takimoto E, Shiojima I, Hiroi Y, (2002) Csx/Nkx2-5 is required for homeostasis and survival of cardiac myocytes in the adult heart. J Biol Chem 277: 24735-24743.