Reduction of misleading (" false" ) positive results in mammalian cell genotoxicity assays. II. Importance of accurate toxicity measurement

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 747, Issue 1, 2012, Pages 104-117

  Fowler, Paul ^a,b   Smith, Robert ^b   Smith, Katie ^b   Young, Jamie ^b   Jeffrey, Laura ^b   Kirkland, David ^c   Pfuhler, Stefan ^d   Carmichael, Paul ^a  

^a UNILEVER RESEARCH   (United Kingdom)

^b UK   (United Kingdom)

^c Kirkland Consulting   (United Kingdom)

^d PROCTER AND GAMBLE COMPANY   (United States)

Author keywords

Genotoxicity; Mammalian cells; Misleading positive results; Toxicity

Indexed keywords

4 NITROPHENOL; ACRYLIC ACID ETHYL ESTER; CASPASE 3; CASPASE 7; CURCUMIN; EUGENOL; GALLIC ACID PROPYL ESTER; RESORCINOL;

ACCURACY; ARTICLE; CELL ASSAY; CELL COUNT; CELL LINE; CELL SURVIVAL; CHO CELL; CONCENTRATION (PARAMETERS); CYTOGENETICS; CYTOTOXICITY; FALSE POSITIVE RESULT; GENOTOXICITY; MAMMAL CELL; MICRONUCLEUS TEST; PRIORITY JOURNAL; SCORING SYSTEM; TOXICITY TESTING;

ANIMALS; APOPTOSIS; CELL COUNT; FALSE POSITIVE REACTIONS; MAMMALS; MICRONUCLEUS TESTS; MUTAGENICITY TESTS;

MAMMALIA; RODENTIA;

EID: 84862331466     PISSN: 13835718     EISSN: 18793592     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2012.04.013     Document Type: Article

References (26)

1. Scott D., Galloway S.M., Marshall R.R., Ishidate M., Brusick D., Ashby J., Myhr B.C. Genotoxicity under extreme culture conditions. A report from ICPEMC Task Group 9. Mutat. Res. 1991, 257:147-204.
2. Directive 2003/15/EC (amending Council Directive 76/768/EEC on the approximation of the laws of the Member States relating to cosmetic products). http://ec.europa.eu/consumers/sectors/cosmetics/files/doc/200315/200315_en.pdf.
3. Kirkland D., Aardema M., Henderson L., Müller L. Evaluation of the ability of a battery of 3 in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens. I. Sensitivity, specificity and relative predictivity. Mutat. Res. 2005, 584:1-256.
4. P. Fowler, K. Smith, J. Young, L. Jeffrey, D. Kirkland, S. Pfuhler, P. Carmichael, Reduction of misleading (" false" ) positive results in mammalian cell genotoxicity assays. I. Choice of cell type, Mutation Research, submitted for publication.
5. Lorge E., Hayashi M., Albertini S., Kirkland D. Comparison of different methods for an accurate assessment of cytotoxicity in the in vitro micronucleus test. I. Theoretical aspects. Mutat. Res. 2008, 655:1-3.
6. Fellows M.D., O'Donovan M.R., Lorge E., Kirkland D. Comparison of different methods for an accurate assessment of cytotoxicity in the in vitro micronucleus test. II. Practical aspects with toxic agents. Mutat. Res. 2008, 655:4-21.
7. Galloway S., Lorge E., Aardema M.J., Eastmond D., Fellows M., Heflich R., Kirkland D., Levy D.D., Lynch A.M., Marzin D., Morita T., Schuler M., Speit G. Workshop summary: top concentration for in vitro mammalian cell genotoxicity assays; and report from working group on toxicity measures and top concentration for in vitro cytogenetics assays (chromosome aberrations and micronucleus). Mutat. Res. 2011, 723:77-83.
8. OECD, 'Genetic Toxicology: OECD Guideline for the Testing of Chemicals. Guideline 487: In Vitro Mammalian Cell Micronucleus Test, 2010.
9. Cytotoxicity measures in the in vitro micronucleus test. Mutat. Res. 2010, 702:131-248. (special issue).
10. Kirkland D., Kasper P., Müller L., Corvi R., Speit G. Recommended lists of genotoxic and non-genotoxic chemicals for assessment of the performance of new or improved genotoxicity tests: a follow-up to an ECVAM workshop. Mutat. Res. 2008, 653:99-108.
11. Richardson C., Williams D.A., Allen J.A., Amphlett G., Chanter D.O., Phillips B. Analysis of data from in vitro cytogenetic assays. " Statistical Evaluation of Mutagenicity Test Data" (UKEMS Guidelines Sub-committee Report, Part III) 1989, 141-154. Cambridge University Press. D.J. Kirkland (Ed.).
12. Xia F., Wang X., Wang Y.H., Tsang N.M., Yandell D.W., Kelsey K.T., Liber H.L. Altered p53 status correlates with differences in sensitivity to radiation-induced mutation and apoptosis in two closely related human lymphoblast lines. Cancer Res. 1995, 55(1):12-15.
13. Mientières S., Biola A., Pallardy M., Marzin D. Using CTLL-2 and CTLL-2 bcl2 cells to avoid interference by apoptosis in the in vitro micronucleus test. Environ. Mol. Mutagen. 2003, 41:14-27.
14. Heddle J.A., Cimino M.C., Hyashi M., Romagna F., Shelby M.D., Tucker J.D., Vanparys Ph., MacGregor J.T. Micronuclei as an index of cytogenetic damage: past, present and future. Environ. Mol. Mutagen. 1991, 18:277-291.
15. Kirkland D. Evaluation of different cytotoxic and cytostatic measures for the in vitro micronucleus test (MNVit): summary of results in the collaborative trial. Mutat. Res. 2010, 702:139-147.
16. Banerjee A., Kunwar A., Mishra B., Priyadarsini K.I. Concentration dependent antioxidant/pro-oxidant activity of curcumin: studies from AAPH induced haemolysis of RBCs. Chem. Biol. Interact. 2008, 174:134-139.
17. Jayaprakasha G.K., Jaganmohan Rao L., Sakariah K.K. Antioxidant activities of curcumin, demethoxycurcumin and bisdemethoxycurcumin. Food Chem. 2006, 98:720-724.
18. Li H., Van Berlo D., Shi T., Speit G., Knaapen A.M., Borm P.J.A., Albrecht C., Schins R.P.F. Curcumin protects against cytotoxic and inflammatory effects of quartz particles but causes oxidative DNA damage in a rat lung epithelial cell line. Toxicol. Appl. Pharmacol. 2008, 227:115-124.
19. Cao J., Jia L., Zhou H., Liu Y., Zhong L. Mitochondrial and nuclear DNA damage induced by curcumin in human hepatoma G2 cells. Toxicol. Sci. 1997, 91:476-483.
20. Zhang P., Omaye S.T. Antioxidant and prooxidant roles for beta-carotene, alpha-tocopherol and ascorbic acid in human lung cells. Toxicol. In Vitro 2001, 15:13-24.
21. Hu T., Miller C., Miller G.M., Aardema M.J. Characterization of p53 in Chinese hamster cell lines CHO-K1, CHO-WBL and CHL: implications for genotoxicity testing. Mutat. Res. 1999, 426:51-62.
22. National Toxicology Programme (NTP) hosted by Department of Health and Human Services (NIEHS) US. http://ntp-server.niehs.nih.gov/.
23. +/- mouse lymphoma specific gene and chromosomal mutation assay: a phase III report of the U.S. environmental protection agency Gene-Tox program. Mutat. Res. 1997, 394:177-303.
24. Loveday K.S., Anderson B.E., Resnick M.A., Zeiger E. Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro V: results with 46 chemicals. Environ. Mol. Mutagen. 1990, 16:272-303.
25. Galloway S.M., Armstrong M.J., Reuben C., Colman S., Brown B., Cannon C., Bloom A.D., Nakamura F., Ahmed M., Duk S., Rimpo J., Margolin B.H., Resnick M.A., Anderson B., Zeiger E. Chromosome aberrations and sister chromatid exchanges in Chinese hamster ovary cells: evaluations of 108 chemicals. Environ. Mol. Mutagen. 1987, 10(Suppl. 10):1-175.
26. Ishidate M., Harnois M., Sofuni T. A comparative analysis of data on the clastogenicity of 951 chemical substances tested in mammalian cell cultures. Mutat. Res. 1988, 195:151-213.