Quantifying amyloid beta (Aβ)-mediated changes in neuronal morphology in primary cultures: Implications for phenotypic screening

Journal of Biomolecular Screening

Volumn 17, Issue 6, 2012, Pages 835-842

  Nguyen, Lan ^a   Wright, Sarah ^b   Lee, Mike ^c   Ren, Zhao ^a   Sauer, John Michael ^d   Hoffman, Wherly ^a   Zago, Wagner ^a   Kinney, Gene G ^a   Bova, Michael P ^a  

^a ELAN PHARMACEUTICALS   (United States)

^b IPierian   (United States)

^c Gilead Pharmaceuticals   (United States)

^d Veterinary Services   (United States)

Author keywords

Alzheimer's disease; high content analysis; neuronal profiling; phenotypic screening

Indexed keywords

AMYLOID BETA PROTEIN; SYNAPSIN;

ALZHEIMER DISEASE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; CELL ASSAY; CELL DAMAGE; CELL STRUCTURE; CONTROLLED STUDY; CYTOTOXICITY; HIPPOCAMPUS; HUMAN; HUMAN CELL; IN VITRO STUDY; NERVE CELL; NERVE CELL CULTURE; NEURITE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RAT; STAINING;

EID: 84862217753     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057112441972     Document Type: Article

References (19)

1. Terry R., Hansen L., Masliah E. Alzheimer's Disease. Terry R. Katzman R., ed. New York: Raven Press ; 1994: 179-196.
2. Selkoe D. J.. Toward a Comprehensive Theory for Alzheimer's Disease. Hypothesis: Alzheimer's Disease Is Caused by the Cerebral Accumulation and Cytotoxicity of Amyloid Beta-Protein. Ann. N.Y. Acad. Sci. 2000 ; 924: 17-25
3. Walsh D. M., Selkoe D. J.. Aβ Oligomer: A Decade of Discovery. J. Neurochem. 2007 ; 101: 1172-1184
4. Crews L., Masliah E.. Molecular Mechanisms of Neurodegeneration in Alzheimer's Disease. Hum. Mol. Genet. 2010 ; 19: 12-20
5. Wright S., Malinin N. L., Powell K. A., Yednock T., Rydel R. E., Griswold-Prenner I.. α2β1 and αVβ1 Integrin Signaling Pathways Mediate Amyloid-β-Induced Neurotoxicity. Neurobiol. Aging. 2007 ; 28: 226-237
6. Seyb K. I., Schuman E. R., Ni J., Huang M., Michaelis M. L., Glickman M. A.. Identification of Small Molecule Inhibitors of β-Amyloid Cytotoxicity through a Cell-Based High-Throughput Screening Platform. J. Biomol. Screen. 2008 ; 13: 870-878
7. Dasilva K. A., Shaw J. E., McLaurin J.. Amyloid-Beta Fibrillogenesis: Structural Insight and Thereapeutic Intervention. Exp. Neurol. 2010 ; 223: 311-321
8. Daub A., Sharma P., Finkbeiner S.. High-Content Screening of Primary Neurons: Ready for Prime Time. Curr. Opin. Neurobiol. 2009 ; 19: 537-543
9. Wogulis M., Wright S., Cunningham D., Chilcote T., Powell K., Rydel R. E.. Nucleation Dependent Polymerization Is an Essential Component of Amyloid-Mediated Neuronal Cell Death. J. Neurochem. 2005 ; 25: 1071-1080
10. O'Brien J., Wilson I., Orton T., Pognan F.. Investigation of the Alamar Blue (Resazurin) Fluorescent Dye for the Assessment of Mammalian Cell Cytotoxicity. Eur. J. Biochem. 2000 ; 267: 5421-5426
11. Cheung Z. H., Chin W. H., Chen Y., Ng Y. P., Ip N. Y.. Cdk5 Is Involved in BDNF-Stimulated Dendritic Growth in Hippocampal Neurons. PLOS Biol. 2007 ; 5: 865-877
12. Awasthi A., Matsunaga Y., Yamada T.. Amyloid-Beta Causes Apoptosis of Neuronal Cells via Caspase Cascade, Which Can Be Prevented by Amyloid-Beta-Derived Short Peptides. Exp. Neurol. 2005 ; 196: 282-289
13. Iijima K., Liu A.-S., Hearn S. A., Konsolaki M., Zhong Y.. Dissecting the Pathological Effects of Human Aβ40 and Aβ42 in Drosophila: A Potential Model for Alzheimer's Disease. Proc. Natl. Acad. Sci. U. S. A. 2004 ; 101: 6623-6628
14. Serrano-Pozo A., Willliam C. M., Ferrer I., Uro-Coste E., Delisle M.-B., Maurage C.-A., Hock C., Nitsch R. M., Masliah E., Growdon J. H., Frosch M. P., Hyman B. T.. Beneficial Effect of Human Anti-amyloid-β Active Immunization on Neurite Morphology and Tau Pathology. Brain. 2010 ; 133: 1312-1327
15. Harrill J. A., Robinette B. L., Mundy W. R.. Use of High Content Image Analysis to Detect Chemical-Induced Changes in Synaptogenesis In Vitro. Toxicol. In Vitro. 2011 ; 25: 368-387
16. Wright K. T., Griffiths G. J., Johnson W. E. B.. A Comparison of High-Content Screening versus Manual Analysis to Assay the Effects of Mesenchymal Stem Cell-Conditioned Medium on Neurite Outgrowth In Vitro. J. Biomol. Screen. 2010 ; 15: 576-582
17. Evans N. A., Facci L., Owen D. E., Soden P. E., Burbidge S. A., Prinjha R. K., Richardson J. C., Skaper S. D.. Aβ1-42 Reduces Synapse Number and Inhibits Neurite Outgrowth in Primary Cortical and Hippocampal Neurons: A Quantitative Analysis. J. Neurosci. Methods. 2008 ; 175: 96-103
18. Hu M., Schurdak M. E., Puttfarcken P. S., El Kouhen R., Gopalakrishnan M., Li J.. High Content Screen Microscopy Analysis of Aβ1-42-Induced Neurite Outgrowth Reduction in Rat Primary Cortical Neurons: Neuroprotective Effects of α7 Nicotinic Acetylcholine Receptor Ligands. Brain Res. 2007 ; 1151: 227-235
19. Wang D., Lagerstrom R., Sun C., Bishof L., Valotton P., Gotte M.. Automated Neurite Outgrowth Analysis. J. Biomol. Screen. 2010 ; 15: 1165-1170