In vitro loading of human cohesin on DNA by the human Scc2-Scc4 loader complex

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 24, 2012, Pages 9366-9371

  Bermudez, Vladimir P ^a   Farina, Andrea ^a   Higashi, Torahiko L ^b   Du, Fang ^a   Tappin, Inger ^a   Takahashi, Tatsuro S ^b   Hurwitz, Jerard ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Cell cycle; Cohesin stability; Cohesion establishment; Sister chromatid cohesion

Indexed keywords

COHESIN; COHESIN 2; COHESIN 4; DOUBLE STRANDED DNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY SPECIFICITY; ARTICLE; CELL CYCLE; CHROMATIN; COMPLEX FORMATION; DNA PROTEIN COMPLEX; EXTRACTION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN SUBUNIT; SISTER CHROMATID; XENOPUS;

ANIMALS; CELL CYCLE; CELL CYCLE PROTEINS; CHROMATIN; CHROMOSOMAL PROTEINS, NON-HISTONE; DIMERIZATION; DNA; HUMANS; XENOPUS;

EID: 84862202409     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1206840109     Document Type: Article

References (31)

1. Onn I, Heidinger-Pauli JM, Guacci V, Unal E, Koshland DE (2008) Sister chromatid cohesion: a simple concept with a complex reality. Annu Rev Cell Dev Biol 24:105-129.
2. Gruber S, Haering CH, Nasmyth K (2003) Chromosomal cohesin forms a ring. Cell 112:765-777. (Pubitemid 36378881)
3. Nasmyth K, Haering CH (2009) Cohesin: its roles and mechanisms. Annu Rev Genet 43:525-558.
4. Takahashi TS, Yiu P, Chou MF, Gygi S, Walter JC (2004) Recruitment of Xenopus Scc2 and cohesin to chromatin requires the pre-replication complex. Nat Cell Biol 6:991-996. (Pubitemid 39359852)
5. Nasmyth K (2011) Cohesin: a catenase with separate entry and exit gates? Nat Cell Biol 13:1170-1177.
6. Uhlmann F (2009) A matter of choice: the establishment of sister chromatid cohesion. EMBO Rep 10:1095-1102.
7. Nishiyama T, et al. (2010) Sororin mediates sister chromatid cohesion by antagonizing Wapl. Cell 143:737-749.
8. Kim BJ, et al. (2010) Genome-wide reinforcement of cohesin binding at pre-existing cohesin sites in response to ionizing radiation in human cells. J Biol Chem 285:22784-22792.
9. Ström L, Lindroos HB, Shirahige K, Sjögren C (2004) Postreplicative recruitment of cohesin to double-strand breaks is required for DNA repair. Mol Cell 16:1003-1015.
10. Sjögren C, Ström L (2010) S-phase and DNA damage activated establishment of sister chromatid cohesion - importance for DNA repair. Exp Cell Res 316:1445-1453.
11. Dorsett D (2011) Cohesin: genomic insights into controlling gene transcription and development. Curr Opin Genet Dev 21:199-206.
12. Mannini L, Menga S, Musio A (2010) The expanding universe of cohesin functions: a new genome stability caretaker involved in human disease and cancer. HumMutat 31:623-630.
13. Strachan T (2005) Cornelia de Lange Syndrome and the link between chromosomal function, DNA repair and developmental gene regulation. Curr Opin Genet Dev 15:258-264.
14. Watrin E, et al. (2006) Human Scc4 is required for cohesin binding to chromatin, sister-chromatid cohesion, and mitotic progression. Curr Biol 16:863-874.
15. Ciosk R, et al. (2000) Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins. Mol Cell 5:243-254. (Pubitemid 30628083)
16. Lengronne A, et al. (2004) Cohesin relocation from sites of chromosomal loading to places of convergent transcription. Nature 430:573-578. (Pubitemid 38998632)
17. Takahashi TS, Basu A, Bermudez V, Hurwitz J, Walter JC (2008) Cdc7-Drf1 kinase links chromosome cohesion to the initiation of DNA replication in Xenopus egg extracts. Genes Dev 22:1894-1905. (Pubitemid 352008638)
18. Siegel LM, Monty KJ (1966) Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases. Biochim Biophys Acta 112:346-362.
19. Gillespie PJ, Hirano T (2004) Scc2 couples replication licensing to sister chromatid cohesion in Xenopus egg extracts. Curr Biol 14:1598-1603. (Pubitemid 39290060)
20. Walter J, Sun L, Newport J (1998) Regulated chromosomal DNA replication in the absence of a nucleus. Mol Cell 1:519-529. (Pubitemid 128374688)
21. Edwards MC, et al. (2002) MCM2-7 complexes bind chromatin in a distributed pattern surrounding the origin recognition complex in Xenopus egg extracts. J Biol Chem 277:33049-33057. (Pubitemid 34984820)
22. Rowles A, Tada S, Blow JJ (1999) Changes in association of the Xenopus origin recognition complex with chromatin on licensing of replication origins. J Cell Sci 112:2011-2018. (Pubitemid 29338825)
23. Gerlich D, Koch B, Dupeux F, Peters JM, Ellenberg J (2006) Live-cell imaging reveals a stable cohesin-chromatin interaction after but not before DNA replication. Curr Biol 16:1571-1578. (Pubitemid 44142999)
24. Higashi TL, et al. (2012) The pre-replication complex recruits XEco2 to chromatin to promote cohesin acetylation in Xenopus extracts. Curr Biol, 10.1016/j.cub.2012.04.013.
25. Pacek M, Walter JC (2004) A requirement for MCM7 and Cdc45 in chromosome unwinding during eukaryotic DNA replication. EMBO J 23:3667-3676. (Pubitemid 39336288)
26. Hu B, et al. (2011) ATP hydrolysis is required for relocating cohesin from sites occupied by its Scc2/4 loading complex. Curr Biol 21:12-24.
27. D'Ambrosio C, et al. (2008) Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev 22:2215-2227.
28. Nonaka N, et al. (2002) Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast. Nat Cell Biol 4:89-93. (Pubitemid 34071986)
29. Onn I, Koshland D (2011) In vitro assembly of physiological cohesin/DNA complexes. Proc Natl Acad Sci USA 108:12198-12205.
30. Wendt KS, Peters JM (2009) How cohesin and CTCF cooperate in regulating gene expression. Chromosome Res 17:201-214.
31. Boos D, Frigola J, Diffley JF (2012) Activation of the replicative DNA helicase: breaking up is hard to do. Curr Opin Cell Biol, 10.1016/j.ceb.2012.01. 011.