A combinatorial approach for directing the amount of fibronectin fibrils assembled by cells that uses surfaces derivatized with mixtures of fibronectin and cell binding domains

Biotechnology Progress

Volumn 28, Issue 3, 2012, Pages 862-871

  Kshatriya, Pradnya P ^a   Karuri, Stella W ^b   Chiang, Chunyi ^a   Karuri, Nancy W ^a  

^a Illinois Institute of Technology   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Cell binding domain; Extracellular matrix; Fibronectin; Fibronectin binding domain; Fibronectin fibril

Indexed keywords

BINDING DOMAIN; CELL-BINDING DOMAIN; EXTRACELLULAR MATRICES; FIBRONECTIN; FIBRONECTIN FIBRIL;

CELL CULTURE; ENZYME ACTIVITY; LOADING; MIXTURES; TISSUE;

CELLS;

FIBRONECTIN; GLUTATHIONE TRANSFERASE;

ANIMAL; ARTICLE; CELL CULTURE; CELL STRAIN 3T3; COMBINATORIAL CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; METABOLISM; MOUSE; PROTEIN BINDING;

ANIMALS; CELLS, CULTURED; COMBINATORIAL CHEMISTRY TECHNIQUES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FIBRONECTINS; GLUTATHIONE TRANSFERASE; MICE; NIH 3T3 CELLS; PROTEIN BINDING;

EID: 84862199593     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1537     Document Type: Article

References (31)

1. Hedman K, Kurkinen M, Alitalo K, Vaheri A, Johansson S, Hook M. Isolation of the pericellular matrix of human fibroblast cultures. J Cell Biol. 1979; 81: 83-91.
2. Hedman K, Johansson S, Vartio T, Kjellen L, Vaheri A, Hook M. Structure of the pericellular matrix: association of heparan and chondroitin sulfates with fibronectin-procollagen fibers. Cell. 1982; 28: 663-671.
3. Yates CC, Bodnar R, Wells A. Matrix control of scarring. Cell Mol Life Sci. 2011; 68: 1871-1881.
4. Wysocki AB, Grinnell F. Fibronectin profiles in normal and chronic wound fluid. LabInvest. 1990; 63: 825-831.
5. Herrick SE, Ireland GW, Simon D, McCollum CN, Ferguson MW. Venous ulcer fibroblasts compared with normal fibroblasts show differences in collagen but not fibronectin production under both normal and hypoxic conditions. J Invest Dermatol. 1996; 106: 187-193.
6. Agren MS, Werthen M. The extracellular matrix in wound healing: a closer look at therapeutics for chronic wounds. Int J Low Extrem Wounds. 2007; 6: 82-97.
7. Singh P, Carraher C, Schwarzbauer JE. Assembly of fibronectin extracellular matrix. Annu Rev Cell Dev Biol. 2010; 26: 397-419.
8. McDonald JA, Quade BJ, Broekelmann TJ, LaChance R, Forsman K, Hasegawa E, Akiyama S. Fibronectin's cell-adhesive domain and an amino-terminal matrix assembly domain participate in its assembly into fibroblast pericellular matrix. J Biol Chem. 1987; 262: 2957-2967.
9. Sechler JL, Rao H, Cumiskey AM, Vega-Colon I, Smith MS, Murata T, Schwarzbauer JE. A novel fibronectin binding site required for fibronectin fibril growth during matrix assembly. J Cell Biol. 2001; 154: 1081-1088.
10. Ohashi T, Kiehart DP, Erickson HP. Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein. Proc Natl Acad Sci USA. 1999; 96: 2153-2158.
11. Aguirre KM, McCormick R, Schwarzbauer JE. Fibronectin self-association is mediated by complementary sites within the amino-terminal one-third of the molecule. J Biol Chem. 1994; 269: 27863-27868.
12. Hocking DC, Sottile J, McKeown-Longo PJ. Fibronectin's III-1 module contains a conformation-dependent binding site for the amino-terminal region of fibronectin. J Biol Chem. 1994; 269: 19183-19187.
13. Hocking DC, Kowalski K. A cryptic fragment from fibronectin's III1 module localizes to lipid rafts and stimulates cell growth and contractility. J Cell Biol. 2002; 158: 175-184.
14. Chernousov MA, Fogerty FJ, Koteliansky VE, Mosher DF. Role of the I-9 and III-1 modules of fibronectin in formation of an extracellular fibronectin matrix. J Biol Chem. 1991; 266: 10851-10858.
15. Chiang C, Karuri SW, Kshatriya PP, Schwartz J, Schwarzbauer J, Karuri NW. A surface derivatization strategy for combinatorial analysis of cell response to mixtures of protein domains. Langmuir. 2011; 28: 548-556.
16. Abraham M. Equilibrium contact angles: theory and measurement. Colloids Surf A. 1996; 116: 55-61.
17. Karuri NW, Lin Z, Rye HS, Schwarzbauer JE. Probing the conformation of the fibronectin III1-2 domain by fluorescence resonance energy transfer. J Biol Chem. 2009; 284: 3445-3452.
18. Royle JA, Nychka D. An algorithm for the construction of spatial coverage designs with implementation in SPLUS. Comput Geosci. 1998; 24: 479-488.
19. Lam RLH, Welch WJ, Young SS. Uniform coverage designs for molecule selection. Technometrics. 2002; 44: 99-109.
20. Furrer R, Nychka D, Sain S. R Package Fields, Version 6.3. Available at:
21. Midwood KS, Wierzbicka-Patynowski I, Schwarzbauer JE. Preparation and analysis of synthetic multicomponent extracellular matrix. Methods Cell Biol. 2002; 69: 145-161.
22. Green JA, Berrier AL, Pankov R, Yamada KM. Beta1 integrin cytoplasmic domain residues selectively modulate fibronectin matrix assembly and cell spreading through talin and Akt-1. J Biol Chem. 2009; 284: 8148-8159.
23. Lemmon CA, Chen CS, Romer LH. Cell traction forces direct fibronectin matrix assembly. Biophys J. 2009; 96: 729-738.
24. Wu C, Bauer JS, Juliano RL, McDonald JA. The alpha 5 beta 1 integrin fibronectin receptor, but not the alpha 5 cytoplasmic domain, functions in an early and essential step in fibronectin matrix assembly. J Biol Chem. 1993; 268: 21883-21888.
25. Wierzbicka-Patynowski I, Schwarzbauer JE. The ins and outs of fibronectin matrix assembly. J Cell Sci. 2003; 116: 3269-3276.
26. Xu J, Bae E, Zhang Q, Annis DS, Erickson HP, Mosher DF. Display of cell surface sites for fibronectin assembly is modulated by cell adherence to 1F3 and C-terminal modules of fibronectin. PLoS One. 2009; 4: 1-12.
27. Ali O, Guillou H, Destaing O, Albiges-Rizo C, Block MR, Fourcade B. Cooperativity between integrin activation and mechanical stress leads to integrin clustering. Biophys J. 2011; 100: 2595-2604.
28. Kornberg L, Earp HS, Parsons JT, Schaller M, Juliano RL. Cell adhesion or integrin clustering increases phosphorylation of a focal adhesion-associated tyrosine kinase. J Biol Chem. 1992; 267: 23439-23442.
29. Slater JH, Frey W. Nanopatterning of fibronectin and the influence of integrin clustering on endothelial cell spreading and proliferation. J Biomed Mater Res A. 2008; 87: 176-195.
30. Wang W, Zhu J, Springer TA, Luo BH. Tests of integrin transmembrane domain homo-oligomerization during integrin ligand binding and signaling. J Biol Chem. 2011; 286: 1860-1867.
31. Pankov R, Cukierman E, Katz BZ, Matsumoto K, Lin DC, Lin S, Hahn C, Yamada KM. Integrin dynamics and matrix assembly: tensin-dependent translocation of alpha(5)beta(1) integrins promotes early fibronectin fibrillogenesis. J Cell Biol. 2000; 148: 1075-1090.