Venous ulcer fibroblasts compared with normal fibroblasts show differences in collagen but not fibronectin production under both normal and hypoxic conditions

Journal of Investigative Dermatology

Volumn 106, Issue 1, 1996, Pages 187-193

  Herrick, Sarah E ^a   Ireland, Grenham W ^a   Simon, Debbie ^b   McCollum, Charles N ^b   Ferguson, Mark W J ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b MANCHESTER UNIVERSITY NHS FOUNDATION TRUST   (United Kingdom)

Author keywords

In vitro; Matrix; Oxygen

Indexed keywords

COLLAGEN; FIBRONECTIN;

ARTICLE; CONTROLLED STUDY; FIBROBLAST; HUMAN; HUMAN CELL; HYPOXIA; PRIORITY JOURNAL; ULCER;

EID: 0030033745     PISSN: 0022202X     EISSN: None     Source Type: Journal    
DOI: 10.1111/1523-1747.ep12329920     Document Type: Article

References (47)

1. Clark RAF: Overview and general considerations of wound repair. In: Clark RAF, Henson PM (eds.). The Molecular and Cellular Biology of Wound Repair. Plenum, New York, pp. 3-33, 1988
2. Regan MC, Kirk SJ, Wasserkrug BA, Barbul A: The wound environment as a regulator of fibroblast phenotype. J Surg Res 50:442-448, 1991
3. Browse NL: The pathogenesis of venous ulceration: a hypothesis. J Vasc Surg 7:468-472, 1988
4. Herrick SE, Sloan P, McGurk M, Freak L, McCollum CN, Ferguson MWJ: Sequential changes in the histological pattern and extracellular matrix deposition during the healing of chronic venous ulcers. Am J Pathol 141:1085-1095, 1992
5. Rasmussen LH, Jensen LT, Avnstorp C, Karlsmark T, Peters K, Hørslev-Petersen K: Collagen types I and III propeptides as markers of healing in chronic leg ulcers. Ann Surg 216:684-691, 1992
6. Mustoe TA, Pierce GF, Morishima C, Deuel TF: Growth factor-induced acceleration of tissue repair through direct and inductive activities in a rabbit dermal ulcer model. J Clin Invest 87:694-703, 1991
7. Angel MF, Kamasastry SS, Swartz WM, Basford RE, Futrell JW: The cause of skin ulcerations associated with venous insufficiency: a unifying hypothesis. Plastic Recon Surg 79:289-297, 1987
8. Falanga V, McKenzie A, Eaglstein WH: Heterogeneity in oxygen diffusion around venous ulcers. J Dermatol Surg Oncol 17:336-339, 1991
9. Dodd HJ, Gaylarde PM, Sarkany I: Skin oxygen tension in venous insufficiency of the lower leg. J Roy Soc Med 78:373-376, 1985
10. Falanga V, Moosa HH, Nemeth AJ, Alstadt SP, Eaglstein WH: Dermal pericapillary fibrin in venous disease and venous ulceration. Arch Dermatol 123:620-623, 1987
11. Clyne CAC, Ramsden WH, Chant ADB, Webster JHH: Oxygen tension on the skin of the gaiter area of limbs with venous disease. Br J Surg 72:644- 647, 1985
12. Mani R, White JE, Barrett DF, Weaver PW: Tissue oxygenation, venous ulcers and fibrin cuffs. J Roy Soc Med 82:345-346, 1989
13. Niinikoski J: Effect of oxygen supply on wound healing and formation of experimental granulation tissue. Acta Physiol Scand 334(suppl):1-72, 1969
14. Stephens FO, Hunt TK: Effect of changes in inspired oxygen and carbon dioxide tensions on wound tensile strength. Ann Surg 173:515-519, 1971
15. Hunt TK, Pai MP: The effect of varying ambient oxygen tensions on wound metabolism and collagen synthesis. Surg Gynecol Obstet 135:561-567, 1972
16. Kivisaari J, Niinikoski J: Effects of hyperbaric oxygenation and prolonged hypoxia on healing of open wounds. Acta Chir Scand 141:14-19, 1975
17. Falanga V: Chronic wounds: pathophysiologic and experimental considerations. J Invest Dermatol 100:721-725, 1993
18. Chvapil M, Hurych J, Ehrlichova E: The influence of various oxygen tensions upon proline hydroxylation and the metabolism of collagenous and non-collagenous proteins in skin slices. Z Physiol Chem 349:211-217, 1968
19. Uitto J, Prockop DJ: Synthesis and secretion of underhydroxylated procollagen at various temperatures by cells subject to temporary anoxia. Biochem Biophys Res Commun 60:414-423, 1974
20. Falanga V, Martin TA, Takagi H, Kirsner RS, Helfman T, Pardes J, Ochoa M: Low oxygen tension increases mRNA levels of alpha 1 (I) procollagen in human dermal fibroblasts. J Cell Physiol 157:408-412, 1993
21. Silver IA: The physiology of wound healing. In: Hunt TK (ed.). Wound Healing and Wound infection. Theory and Surgical Practice. Appleton-Century Crofts, New York, pp 11-31, 1980
22. Jönsson K, Jensen JA, Goodson WH, Scheuenstuhl H, West J, Williams Hopf H, Hunt TK: Tissue oxygenation, anaemia and perfusion in relation to wound healing in surgical patients. Ann Surg 214:605-613, 1991
23. Falanga V, Kirsner RS: Low oxygen stimulates proliferation of fibroblasts seeded as single cells. J Cell Physiol 154:506-510, 1993
24. Balin AK, Fisher AJ, Carter DM: Oxygen modulates growth of human cells at physiological partial pressures. J Exp Med 160:152-166, 1984
25. Knighton DR, Hunt TK, Scheuenstuhl H, Halliday B, Werb Z, Banda MJ: Oxygen tension regulates the expression of angiogenesis factor by macrophages. Science 221:1283-1285, 1983
26. Falanga V, Qian SW, Danielpour D, Katz MH, Roberts AB, Sporn MB: Hypoxia upregulates the synthesis of TGF-beta 1 by human dermal fibroblasts. J Invest Dermatol 97:634-637, 1991
27. Kourembanas S, Hannan RL, Faller DV: Oxygen tension regulates the expression of the platelet-derived growth factor-B chain gene in human endothelial cells. J Clin Invest 86:670-674, 1990
28. Helfman T, Falanga V: Gene expression in low oxygen tension. Am J Med Sci 306:37-41, 1993
29. Rosen P, Boulton M, Moriarty P, Khaliq A, McLeod D: Effect of varying oxygen concentrations on the proliferation of retinal microvascular cells in vitro. Exp Eye Res 53:597-601, 1991
30. Schor SL, Court J: Different mechanisms involved in the attachment of cells to native and denatured collagen. J Cell Sci 38:267-281, 1979
31. Peterkofsky B, Diegelmann R: Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry 10:988-994, 1971
32. Peterkofsky B: The effects of ascorbic acid on collagen polypeptide synthesis and proline hydroxylation during the growth of cultured fibroblasts. Arch Biochem Biophys 152:318-328, 1972
33. Engvall ES, Ruoslahti E: Binding of soluble form of fibroblast surface protein, fibronectin, to collagen. Int J Cancer 20:1-5, 1977
34. 4. Nature 227:680-685, 1970
35. Bonner WM, Laskey RA: A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem 46:83-88, 1974
36. Diegelmann RF, Cohen K, McCoy BJ: Growth kinetics and collagen synthesis of normal skin, normal scar and keloid fibroblasts in vitro. J Cell Physiol 98:341-346, 1979
37. Fleischmajer R, Perlish JS, Krieg T, Timpl R: Variability in collagen and fibronectin synthesis by scleroderma fibroblasts in primary culture. J Invest Dermatol 76:400-403, 1981
38. Russel SB, Trupin KM, Rodriguez-Eaton S, Russel JD, Trupin JS: Reduced growth-factor requirement of keloid-derived fibroblasts may account for tumor growth. Proc Natl Acad Sci USA 85:587-591, 1988
39. Postlethwaite AE, Smith GN, Mainardi CL, Seyer JM, Kang AH: Lymphocyte modulation of fibroblast function in vitro: stimulation and inhibition of collagen production by different effector molecules. J Immunol 132:2470-2477, 1984
40. Nusgens B, Merrill C, Lapiere C, Bell E: Collagen biosynthesis by cells in a tissue equivalent matrix in vitro. Col Rel Res 4:351-364, 1984
41. Grinnell F, Ho CH, Wysocki A: Degradation of fibronectin and vitronectin in chronic wound fluid; analysis by cell blotting, immunoblotting and cell adhesion assays. J Invest Dermatol 984:410-416, 1992
42. Wysocki AB, Staiano-Coico L, Grinneli F; Wound fluid from chronic leg ulcers contains elevated levels of metalloproteinases MMP-2 and MMP-9. J Invest Dermatol 101:64-68, 1993
43. Palolahti M, Lauharanta J, Stephens RW, Kuusela P, Vaheri A: Proteolytic activity in leg ulcer exudate. Exp Dermatol 2:29-37, 1993
44. Niinikoski J, Gottrup F, Hunt TK: The role of oxygen in wound repair. In: Janssen H, Rooman R, Robertson JIS (eds.). Wound Healing. Wrightson, Petersfield, U.K., pp. 165-174, 1991
45. Prockop DJ, Kivirikko KI, Tuderman L, Guzman NA: The biosynthesis of collagen and its disorders. Part 1. N Engl J Med 30:13-23, 1979
46. Comstock JP, Udenfriend S: Effect of lactate on collagen proline hydroxylase activity in cultured L-929 fibroblasts. Proc Natl Acad Sci USA 66:552-557, 1970
47. Roberts AB, Sporn MB, Assoian RK, Smith JM, Roche NS, Wakefield LM, Heine UI, Liotta LA, Falanga V, Kehrl JH, Fauci AS: Transforming growth factor β: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen synthesis in vitro. Proc Natl Acad Sci USA 83:4167-4171, 1986