The Corynebacterium glutamicum cglIM gene encoding a 5-cytosine methyltransferase enzyme confers a specific DNA methylation pattern in an McrBC-deficient Escherichia coli strain

Gene

Volumn 203, Issue 2, 1997, Pages 95-101

  Schäfer, Andreas ^a   Tauch, Andreas ^a   Droste, Nicole ^a   Pühler, Alfred ^a   Kalinowski, Jörn ^a  

^a BIELEFELD UNIVERSITY   (Germany)

Author keywords

Electroporation; McrBC; Methylation restriction; Restriction modification system

Indexed keywords

BACTERIAL DNA; METHYLTRANSFERASE; RESTRICTION ENDONUCLEASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIUM MUTANT; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; DNA METHYLATION; ESCHERICHIA COLI; GENE AMPLIFICATION; MOLECULAR CLONING; NEISSERIA GONORRHOEAE; NONHUMAN; OPERON; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; SOIL MICROFLORA;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CORYNEBACTERIUM; DNA METHYLATION; DNA RESTRICTION ENZYMES; DNA, BACTERIAL; ELECTROPORATION; ESCHERICHIA COLI; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; PLASMIDS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SITE-SPECIFIC DNA METHYLTRANSFERASE (CYTOSINE-SPECIFIC); TRANSFORMATION, BACTERIAL;

EID: 18244431356     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00519-2     Document Type: Article

References (19)

1. Anderson J.E. Restriction endonucleases and modification methylases. Curr. Opin. Struct. Biol. 3:1993;24-30.
2. Grant S.G.N., Jessee J., Bloom F.R., Hanahan D. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. USA. 87:1990;4645-4649.
3. Haynes J.A., Britz M.L. Electrotransformation of Brevibacterium lactofermentum and Corynebacterium glutamicum: growth in Tween 80 increases transformation frequencies. FEMS Microbiol. Lett. 61:1989;329-334.
4. Jang K.-H., Chambers P.J., Britz M.L. Analysis of nucleotide methylation in DNA from Corynebacterium glutamicum and related species. FEMS Microbiol. Lett. 136:1996;309-315.
5. Myers E.W., Miller W. Optimal alignments in linear space. CABIOS. 4:1988;11-17.
6. Posfai J., Bhagwat A.S., Posfai G., Roberts R.J. Predictive motifs derived from cytosine methyltransferases. Nucleic Acids Res. 17:1989;2421-2435.
7. Raleigh E.A. Organization and function of the mcrBC genes of Escherichia coli K-12. Mol. Microbiol. 6:1992;1079-1086.
8. Raleigh E.A., Murray N.E., Revel H., Blumenthal R.M., Westaway D., Reith A.D., Rigby P.W.J., Elhai J., Hanahan D. McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucleic Acids Res. 16:1988;1563-1575.
9. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:1977;5463-5467.
10. Schäfer A., Kalinowski J., Pühler A. Increased fertility of Corynebacterium glutamicum recipients in intergeneric matings with Escherichia coli after stress exposure. Appl. Environ. Microbiol. 60:1994;756-759.
11. Schäfer A., Kalinowski J., Simon R., Seep-Feldhaus A.-H., Pühler A. High-frequency conjugal plasmid transfer from Escherichia coli to various gram-positive coryneform bacteria. J. Bacteriol. 172:1990;1663-1666.
12. Schäfer A., Schwarzer A., Kalinowski J., Pühler A. Cloning and characterization of a DNA region encoding a stress-sensitive restriction system from Corynebacterium glutamicum ATCC 13032 and analysis of its role in intergeneric conjugation with Escherichia coli. J. Bacteriol. 176:1994;7309-7319.
13. Schäfer A., Tauch A., Jäger W., Kalinowski J., Thierbach G., Pühler A. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene. 145:1994;69-73.
14. Staden R. The current status and portability of our sequence handling software. Nucleic Acids Res. 14:1986;217-231.
15. Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A. Restriction and modification systems of Neisseria gonorrhoeae. Gene. 157:1995;19-22.
16. Szybalski W., Blumenthal R.M., Brooks J.E., Hattman S., Raleigh E.A. Nomenclature for bacterial genes coding for class-II restriction endonucleases and modification methyltransferases. Gene. 74:1988;279-280.
17. Tauch A., Kirchner O., Wehmeier L., Kalinowski J., Pühler A. Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS Microbiol. Lett. 123:1994;343-348.
18. Wohlleben, W., Muth, G., Kalinowski, J., 1993. Genetic engineering of gram positive bacteria. In: Rehm, H.-J., Reed, G., Pühler, A., Stadler, P. (Eds), Biotechnology, Vol. 2. VCH, Weinheim, pp. 83-133.
19. Wu J.C., Santi D.V. Kinetic and catalytic mechanism of HhaI methyltransferase. J. Biol. Chem. 262:1987;4778-4786.