A novel approach to attenuate proliferative vitreoretinopathy using ultrasound-targeted microbubble destruction and recombinant adeno-associated virus-mediated RNA interference targeting transforming growth factor-β2 and platelet-derived growth factor-B

Journal of Gene Medicine

Volumn 14, Issue 5, 2012, Pages 339-347

  Zheng, Xiaozhi ^a   Du, Lianfang ^b   Wang, Huiping ^b   Gu, Qing ^b  

^a NANTONG UNIVERSITY   (China)

^b SHANGHAI FIRST PEOPLE S HOSPITAL   (China)

Author keywords

PDGF B; Proliferative vitreoretinopathy; Recombinant adeno associated virus; SiRNA; TGF 2; Ultrasound targeted microbubble destruction

Indexed keywords

MESSENGER RNA; PARVOVIRUS VECTOR; PLATELET DERIVED GROWTH FACTOR B; SMALL INTERFERING RNA; SODIUM CHLORIDE; TRANSFORMING GROWTH FACTOR BETA2;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ECHOGRAPHY; ENZYME LINKED IMMUNOSORBENT ASSAY; EYE FUNDUS; GENE TARGETING; GENE THERAPY; GENETIC TRANSFECTION; HISTOPATHOLOGY; MALE; MICROBUBBLE; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; OPHTHALMOSCOPY; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; ULTRASOUND TARGETED MICROBUBBLE DESTRUCTION; VIRAL GENE DELIVERY SYSTEM; VITREORETINOPATHY;

ANIMALS; DEPENDOVIRUS; GENE TRANSFER TECHNIQUES; GENETIC THERAPY; HUMANS; MALE; MICROBUBBLES; PROTO-ONCOGENE PROTEINS C-SIS; RATS; RATS, WISTAR; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFORMING GROWTH FACTOR BETA2; ULTRASONOGRAPHY, INTERVENTIONAL; VITREORETINOPATHY, PROLIFERATIVE;

ADENO-ASSOCIATED VIRUS; RATTUS;

EID: 84862065730     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.2629     Document Type: Article

References (32)

1. Ikuno Y, Leong FL, Kazlauskas A. Attenuation of experimental proliferative vitreoretinopathy by inhibiting the platelet-derived growth factor receptor. Invest Ophthalmol Vis Sci 2000; 41: 3107-3116.
2. Behar-Cohen FF, Thillaye-Goldenberg B, de Bizemont T, et al. EIU in the rat promotes the potential of syngeneic retinal cells injected into the vitreous cavity to induce PVR. Invest Ophthalmol Vis Sci 2000; 41: 3915-3924.
3. Zhang L, Li XX, Zhao MW, et al. Antisense oligonucleotide targeting c-fos mRNA limits retinal pigment epithelial cell proliferation; a key step in the progression of proliferative vitreoretinopathy. Exp Eye Res 2006; 83: 1405-1411.
4. Zheng XZ, Li HL, Du LF, Gu Q, Wang HP. A rat model of proliferative vitreoretinopathy induced by RPE-J cells and platelet-rich plasma. Asian Biomed 2009; 3: 507-515.
5. Zheng XZ, Du LF, Wang HP. A immunohistochemical analysis of a rat model of proliferative vitreoretinopathy and a comparison of the expression of TGF-β and PDGF among the induction methods. Bosnian J Basic Med 2010; 10: 204-209.
6. Charteris DG. Proliferative vitreoretinopathy: pathobiology, surgical management, and adjunctive treatment. Br J Ophthalmol 1995; 79: 953-960.
7. Nagineni CN, Kutty V, Detrick B, Hooks JJ. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-β2. J Cell Physiol 2005; 203: 35-43.
8. Andrews A, Balciunaite E, Leong FL, et al. Platelet-derived growth factor plays a key role in proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 1999; 40: 2683-2689.
9. Gamulescu MA, Chen Y, He S, et al. Transforming growth factor β2-induced yofibroblastic differentiation of human retinal pigment epithelial cells: regulation by extracellular matrix proteins and hepatocyte growth factor. Exp Eye Res 2006; 83: 212-222.
10. Carrington L, McLeod D, Boulton M. IL-10 and antibodies to TGF-beta2 and PDGF inhibit RPE-mediated retinal contraction. Invest Ophthalmol Vis Sci 2000; 41: 1210-1216.
11. Reich SJ, Fosnot J, Kuroki A, et al. Small interfering RNA (siRNA) targeting VEGF effectively inhibits ocular neovascularization in a mouse model. Mol Vis 2003; 9: 210-216.
12. Otani K, Yamahara K, Ohnishi S, Obata H, Kitamura S, Nagaya N. Nonviral delivery of siRNA into mesenchymal stem cells by a combination of ultrasound and microbubbles. J Control Release 2009; 133: 146-153.
13. Vandenbroucke RE, Lentacker I, Demeester J, De Smedt SC, Sanders NN. Ultrasound assisted siRNA delivery using PEG-siPlex loaded microbubbles. J Control Release 2008; 126: 265-273.
14. Daya S, Berns KI. Gene modification using adeno-associated virus vectors. Clin Microbiol Rev 2008; 21: 583-593.
15. Pang JJ, Lauramore A, Deng WT, et al. Comparative analysis of in vivo and in vitro AAV vector transduction in the neonatal mouse retina: effects of serotype and site of administration. Vision Res 2008; 48: 377-385.
16. Zheng XZ, Wu Y, Li HL, Du LF, Gu Q, Wang HP. Comparative analysis of the effects of ultrasound-targeted microbubble destruction on the recombinant adeno-associated virus and plasmid mediated transgene expression in human retinal pigment epithelium cell. Mol Med Rep 2009; 2: 937-942.
17. Zheng XZ, Li HL, Du LF, Wang HP, Gu Q. Comparative analysis of gene transfer to human and rat retinal pigment epithelium cell line by a combinatorial use of recombinant adeno-associated virus and ultrasound or/and microbubbles. Bosnian J Basic Med 2009; 9: 174-181.
18. Zheng XZ, Li HL, Du LF, Wang HP, Gu Q. In vivo and in vitro effects of ultrasound or/and microbubbles on recombinant adeno-associated virus-mediated transgene expression in the retina. Asian Biomed 2009; 3: 497-506.
19. Wu J, Zhang S, Wu X, et al. Enhanced transduction and improved photoreceptor survival of retinal degeneration by the combinatorial use of rAAV2 with a lower dose of adenovirus. Vision Res 2008; 48: 1648-1654.
20. Li HL, Zheng XZ, Wang HP, Li F, Wu Y, Du LF. Ultrasound-targeted microbubble destruction enhances AAV mediated gene transfection: human RPE cells in vitro and the rat retina in vivo. Gene Ther 2009; 16: 1146-1153.
21. Hosseinkhani H, Aoyama T, Ogawa O, Tabata Y. Ultrasound enhances the transfection of plasmid DNAby non-viral vectors. Curr Pharmaceut Biotechnol 2003; 4: 109-122.
22. Otani K, Yamahara K, Ohnishi S, Obata H, Kitamura S, Nagaya N. Nonviral delivery of siRNA into mesenchymal stem cells by a combination of ultrasound and microbubbles. J Control Release 2009; 133: 146-153.
23. Mukherjee D, Wong J, Griffin B, et al. Ten-fold augmentation of endothelial uptake of vascular endothelial growth factor with ultrasound after systemic administration. J Am Coll Cardiol 2000; 35: 1678-1686.
24. Zderic V, Vaezy S, Martin RW, Clark JI. Ocular drug delivery using 20-kHz ultrasound. Med Biol 2002; 28: 823-829.
25. Howard CM, Forsberg F, Minimo C, Liu JB, Merton DA, Claudio PP. Ultrasound guided site specific gene delivery system using adenoviral vectors and commercial ultrasound contrast agents. J Cell Physiol 2006; 209: 413-421.
26. Shohet RV, Chen S, Zhou YT, et al. Echocardiographic destruction of albumin microbubbles directs gene delivery to the myocardium. Circulation 2000; 101: 2554-2556.
27. Amabile PG, Waugh JM, Lewis TN, Elkins CJ, Janas W, Dake MD. High-efficiency endovascular gene delivery via therapeutic ultrasound. J Am Coll Cardiol 2001; 37: 1975-1980.
28. Juffermans LJ, Dijkmans PA, Musters RJ, Visser CA, Kamp O. Transient permeabilization of cell membranes by ultrasound-exposed microbubbles is related to formation of hydrogen peroxide. Am J Physiol Heart Circ Physiol 2006; 291: H1595-H1601.
29. Basta G, Venneri L, Lazzerini G, et al. In vitro modulation of intracellular oxidative stress of endothelial cells by diagnostic cardiac ultrasound. Cardiovasc Res 2003; 58: 156-161.
30. Basta G, Venneri L, Lazzerini G, et al. Development of safe and efficient novel nonviral gene transfer using ultrasound: enhancement of transfection efficiency of naked plasmid DNA in skeletal muscle. Gene Ther 2002; 9: 372-380.
31. Lu QL, Liang HD, Partridge T, Blomley MJ. Microbubble ultrasound improves the efficiency of gene transduction in skeletal muscle in vivo with reduced tissue damage. Gene Ther 2003; 10: 396-405.
32. Ding W, Zhang L, Yan Z, Engelhardt JF. Intracellular trafficking of adeno-associated viral vectors. Gene Ther 2005; 12: 873-880.