Taking down the FLAG! how insect cell expression challenges an established tag-system

PLoS ONE

Volumn 7, Issue 6, 2012, Pages

  Schmidt, Peter M ^a,b   Sparrow, Lindsay G ^a   Attwood, Rebecca M ^a   Xiao, Xiaowen ^a   Adams, Tim E ^a   McKimm Breschkin, Jennifer L ^a  

^a CSIRO   (Australia)

^b CSL LIMITED   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ENTEROPEPTIDASE; EPITOPE; PROTEIN TYROSINE KINASE; RECOMBINANT PROTEIN; TYROSINE; ANTIBODY; FLAG PEPTIDE; PEPTIDE; PROTEIN TYROSINE SULFOTRANSFERASE; PROTEIN-TYROSINE SULFOTRANSFERASE; SIALIDASE; SULFOTRANSFERASE;

ARTICLE; CATALYSIS; CONTROLLED STUDY; HIGH THROUGHPUT SEQUENCING; INSECT CELL; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SECRETION; SOLUBILITY; SULFATION; AFFINITY CHROMATOGRAPHY; ANIMAL; CELL LINE; CELL STRAIN HEK293; GEL CHROMATOGRAPHY; GENETICS; HUMAN; IMMUNOLOGY; INSECT; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; WESTERN BLOTTING;

ANIMALS; ANTIBODIES; BLOTTING, WESTERN; CELL LINE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; EPITOPES; HEK293 CELLS; HUMANS; INSECTS; MASS SPECTROMETRY; NEURAMINIDASE; PEPTIDES; RECOMBINANT PROTEINS; SULFOTRANSFERASES;

EID: 84862001696     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0037779     Document Type: Article

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