Spontaneous α-N-6-phosphogluconoylation of a 'His tag' in Escherichia coli: The cause of extra mass of 258 or 178 Da in fusion proteins

Analytical Biochemistry

Volumn 267, Issue 1, 1999, Pages 169-184

  Geoghegan, Kieran F ^a   Dixon, Henry B F ^b   Rosner, Philip J ^a   Hoth, Lise R ^a   Lanzetti, Anthony J ^a   Borzilleri, Kris A ^a   Marr, Eric S ^a   Pezzullo, Linda H ^a   Martin, Leroy B ^c   Lemotte, Peter K ^a   McColl, Alexander S ^a   Kamath, Ajith V ^a   Stroh, Justin G ^a  

^a PFIZER INC   (United States)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c Micromass Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLATION; ESCHERICHIA COLI; GLUCOSE; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

DEPHOSPHORYLATIONS; GLUCOSE-6-PHOSPHATE DEHYDROGENASE; HIS-TAGGED PROTEINS; MODIFIED PROTEINS; PERIODATE OXIDATION; PLAUSIBLE MECHANISMS; POST-TRANSLATIONAL MODIFICATIONS; TANDEM MASS SPECTROMETRY;

PROTEINS;

6 PHOSPHOGLUCONOYL 1,5 LACTONE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; HISTIDINE; HYBRID PROTEIN; UNCLASSIFIED DRUG;

ACYLATION; AMINO ACID SUBSTITUTION; ARTICLE; BINDING KINETICS; ESCHERICHIA COLI; NONHUMAN; NUCLEAR MAGNETIC RESONANCE IMAGING; OXIDATION; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN STRUCTURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0033080755     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1998.2990     Document Type: Article

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