Centrifugation assisted microreactor enables facile integration of trypsin digestion, hydrophilic interaction chromatography enrichment, and on-column deglycosylation for rapid and sensitive N-glycoproteome analysis

Analytical Chemistry

Volumn 84, Issue 11, 2012, Pages 5146-5153

  Zhu, Jun ^a   Wang, Fangjun ^a   Chen, Rui ^a   Cheng, Kai ^a   Xu, Bo ^a   Guo, Zhimou ^a   Liang, Xinmiao ^a   Ye, Mingliang ^a   Zou, Hanfa ^a  

^a DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYSIS SYSTEM; DEGLYCOSYLATION; DETECTION LIMITS; GLYCOPEPTIDES; HIGH SENSITIVITY; HUMAN IGG; HUMAN SERUM; HYDROPHILIC INTERACTION CHROMATOGRAPHY; MICRO-REACTOR; N-GLYCOSYLATION SITES; PROTEIN DIGESTION; PROTEIN DIGESTS; PROTEIN SAMPLES; SAMPLE HANDLING; SAMPLE PREPARATION PROCEDURE; SEAMLESS INTEGRATION; SIGNIFICANT IMPACTS; TOTAL PROCESSING TIME; TRIFLUOROACETIC ACIDS; TRYPSIN DIGESTION;

ACETONITRILE; BODY FLUIDS; CENTRIFUGATION; DESALINATION; LIQUID CHROMATOGRAPHY; SAMPLING;

PROTEINS;

ACETONITRILE; ACETONITRILE DERIVATIVE; GLYCOPEPTIDE; IMMUNOGLOBULIN G; PROTEOME; TRIFLUOROACETIC ACID; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; CENTRIFUGATION; CHEMICAL PHENOMENA; CHEMISTRY; GLYCOSYLATION; HUMAN; LIMIT OF DETECTION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; PROTEIN DEGRADATION;

ACETONITRILES; AMINO ACID SEQUENCE; CENTRIFUGATION; CHROMATOGRAPHY, LIQUID; GLYCOPEPTIDES; GLYCOSYLATION; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; IMMUNOGLOBULIN G; LIMIT OF DETECTION; MOLECULAR SEQUENCE DATA; PROTEOLYSIS; PROTEOME; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRIFLUOROACETIC ACID; TRYPSIN;

EID: 84861844024     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac3000732     Document Type: Article

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