Differential gel electrophoresis of the tear proteome

Optometry and Vision Science

Volumn 89, Issue 6, 2012, Pages

  Markoulli, Maria ^a   Papas, Eric ^a,b   Cole, Nerida ^a,b   Holden, Brien ^a,b  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

^b VISION COOPERATIVE RESEARCH CENTRE   (Australia)

Author keywords

contact lenses; differential gel electrophoresis; extended wear; flush tears

Indexed keywords

ASSAY METHODS; BICINCHONINIC ACIDS; CONTACT LENS WEAR; EXTENDED WEARS; FLUSH TEARS; GEL ELECTROPHORESIS; LENS WEAR; NON-CONTACT; PROTEIN SPOTS; PROTEOMIC TECHNIQUES; TIME POINTS; TOTAL PROTEIN;

CONTACT LENSES; ELECTROPHORESIS; MASS SPECTROMETRY;

PROTEINS;

PROTEOME;

ADULT; ARTICLE; CHEMISTRY; COMPARATIVE STUDY; ELECTROPHORESIS; EXTENDED WEAR CONTACT LENS; FEMALE; HUMAN; LACRIMAL FLUID; MALE; METHODOLOGY; REFERENCE VALUE; REPRODUCIBILITY;

ADULT; CONTACT LENSES, EXTENDED-WEAR; ELECTROPHORESIS; FEMALE; HUMANS; MALE; PROTEOME; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; TEARS;

EID: 84861838643     PISSN: 10405488     EISSN: None     Source Type: Journal    
DOI: 10.1097/OPX.0b013e318255dc46     Document Type: Article

References (29)

1. Twyman RM. Principles of Proteomics. New York: BIOS Scientific Publishers; 2004.
2. Endre ZH, Westhuyzen J. Early detection of acute kidney injury: emerging new biomarkers. Nephrology (Carlton) 2008;13:91-8. (Pubitemid 351229631)
3. Ong SE, Pandey A. An evaluation of the use of two-dimensional gel electrophoresis in proteomics. Biomol Eng 2001;18:195-205. (Pubitemid 32939338)
4. Gharbi S, Gaffney P, Yang A, Zvelebil MJ, Cramer R, Waterfield MD, Timms JF. Evaluation of two-dimensional differential gel electrophoresis for proteomic expression analysis of a model breast cancer cell system. Mol Cell Proteomics 2002;1:91-8.
5. Zhou H, Ranish JA, Watts JD, Aebersold R. Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry. Nat Biotechnol 2002;20:512-5. (Pubitemid 34495941)
6. Nichols JJ, Green-Church KB. Mass spectrometry-based proteomic analyses in contact lens-related dry eye. Cornea 2009;28:1109-17.
7. Su S, Liu P, Zhang H, Li Z, Song Z, Zhang L, Chen S. Proteomic analysis of human age-related nuclear cataracts and normal lens nuclei. Invest Ophthalmol Vis Sci 2011;52:4182-91.
8. Joseph R, Srivastava OP, Pfister RR. Differential epithelial and stromal protein profiles in keratoconus and normal human corneas. Exp Eye Res 2011;92:282-98.
9. Unlu M, Morgan ME, Minden JS. Difference gel electrophoresis: a single gel method for detecting changes in protein extracts. Electrophoresis 1997;18:2071-7. (Pubitemid 27501267)
10. Alban A, David SO, Bjorkesten L, Andersson C, Sloge E, Lewis S, Currie I. A novel experimental design for comparative twodimensional gel analysis: two-dimensional difference gel electrophoresis incorporating a pooled internal standard. Proteomics 2003;3: 36-44. (Pubitemid 36151066)
11. Thakur A, Willcox MD. Contact lens wear alters the production of certain inflammatory mediators in tears. Exp Eye Res 2000;70: 255-9. (Pubitemid 30658896)
12. Markoulli M, Papas E, Petznick A, Holden B. Validation of the flush method as an alternative to basal or reflex tear collection. Curr Eye Res 2011;36:198-207.
13. Ehrmann K, Fedtke C, Radić A. Assessment of computer generated vision charts. Cont Lens Anterior Eye 2009;32:133-40.
14. Cornea and Contact Lens Research Unit. CCLRU Grading Scales. Sydney, Australia: University of New South Wales; 1996.
15. Marouga R, David S, Hawkins E. The development of the DIGE system: 2D fluorescence difference gel analysis technology. Anal Bioanal Chem 2005;382:669-78. (Pubitemid 40890994)
16. Zhao Z, Liu J, Wasinger VC, Malouf T, Nguyen-Khuong T, Walsh B, Willcox MD. Tear lipocalin is the predominant phosphoprotein in human tear fluid. Exp Eye Res 2010;90:344-9.
17. Tonge R, Shaw J, Middleton B, Rowlinson R, Rayner S, Young J, Pognan F, Hawkins E, Currie I, Davison M. Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technology. Proteomics 2001;1:377-96. (Pubitemid 33585112)
18. Gatlin CL, Kleemann GR, Hays LG, Link AJ, Yates JR, III. Protein identification at the low femtomole level from silver-stained gels using a new fritless electrospray interface for liquid chromatographymicrospray and nanospray mass spectrometry. Anal Biochem 1998; 263:93-101. (Pubitemid 28445431)
19. Malm C, Hadrevi J, Bergstrom SA, Pedrosa-Domellof F, Antti H, Svensson M, Frangsmyr L. Evaluation of 2-D DIGE for skeletal muscle: protocol and repeatability. Scand J Clin Lab Invest 2008;68: 793-800.
20. Sack RA, Conradi L, Krumholz D, Beaton A, Sathe S, Morris C. Membrane array characterization of 80 chemokines, cytokines, and growth factors in open-and closed-eye tears: angiogenin and other defense system constituents. Invest Ophthalmol Vis Sci 2005;46: 1228-38. (Pubitemid 41685857)
21. Stapleton F, Keay L, Edwards K, Naduvilath T, Dart JK, Brian G, Holden BA. The incidence of contact lens-related microbial keratitis in Australia. Ophthalmology 2008;115:1655-62.
22. Hassan MI, Waheed A, Yadav S, Singh TP, Ahmad F. Zinc alpha 2-glycoprotein: a multidisciplinary protein. Mol Cancer Res 2008;6: 892-906.
23. Molloy MP, Bolis S, Herbert BR, Ou K, Tyler MI, van Dyk DD, Willcox MD, Gooley AA, Williams KL, Morris CA, Walsh BJ. Establishment of the human reflex tear two-dimensional polyacrylamide gel electrophoresis reference map: new proteins of potential diagnostic value. Electrophoresis 1997;18:2811-2815. (Pubitemid 28059953)
24. Versura P, Nanni P, Bavelloni A, Blalock, WL, Piazzi M, Roda A, Campos EC. Tear proteomics in evaporative dry eye disease. Eye 2010;24:1396-1402.
25. Glasson M, Stapleton F, Willcox M. Lipid, lipase and lipocalin differences between tolerant and intolerant contact lens wearers. Curr Eye Res 2002;25:227-35. (Pubitemid 36307988)
26. Yamada M, Mochizuki H, Kawashima M, Hata S. Phospholipids and their degrading enzyme in the tears of soft contact lens wearers. Cornea 2006;25:S68-72.
27. Choy CK, Cho P, Benzie IF, Ng V. Effect of one overnight wear of orthokeratology lenses on tear composition. Optom Vis Sci 2004;81: 414-20. (Pubitemid 38780435)
28. Stapleton F, Willcox MD, Morris CA, Sweeney DF. Tear changes in contact lens wearers following overnight eye closure. Curr Eye Res 1998;17:183-8. (Pubitemid 28073784)
29. Masinick SA, Montgomery CP, Montgomery PC, Hazlett LD. Secretory IgA inhibits Pseudomonas aeruginosa binding to cornea and protects against keratitis. Invest Ophthalmol Vis Sci 1997;38: 910-8. (Pubitemid 27193517)