Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1824, Issue 8, 2012, Pages 954-962

  Mizutani, Kimihiko ^a   Toyoda, Mayuko ^a   Otake, Yuichiro ^a   Yoshioka, Soshi ^a   Takahashi, Nobuyuki ^a   Mikami, Bunzo ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Expression system; Pichia pastoris; Secretory protein; X ray structure

Indexed keywords

AMYLASE; RECOMBINANT ENZYME; SECRETORY PROTEIN;

ARTICLE; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME STRUCTURE; HIGH THROUGHPUT SEQUENCING; HOMOLOGOUS RECOMBINATION; HYDROLYSIS; NONHUMAN; ORYZIAS; PH; PICHIA PASTORIS; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; TEMPERATURE; X RAY CRYSTALLOGRAPHY;

ALPHA-AMYLASES; AMINO ACID SEQUENCE; ANIMALS; CHLORIDES; CRYSTALLOGRAPHY, X-RAY; FISH PROTEINS; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; ORYZIAS; PICHIA; PROTEIN DENATURATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; TEMPERATURE;

ORYZIINAE; PICHIA PASTORIS; SUIDAE; VERTEBRATA;

EID: 84861777272     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2012.05.005     Document Type: Article

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