Structural and functional characterization of recombinant human serum transferrin secreted from pichia pastoris

Bioscience, Biotechnology and Biochemistry

Volumn 74, Issue 2, 2010, Pages 309-315

  Mizutani, Kimihiko ^a   Hashimoto, Kazuhiko ^a   Takahashi, Nobuyuki ^a   Hirose, Masaaki ^a   Aibara, Shigeo ^a   Mikami, Bunzo ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Carbohydrate chain; Iron transport; Pichia pastoris; Transferrin

Indexed keywords

BLOOD SERUM; CARBOHYDRATE CHAINS; CARBOHYDRATE MODIFICATION; CIRCULAR DICHROISM SPECTRA; CULTURE MEDIUM; ELECTROPHORETIC BEHAVIOR; FLUORESCENCE SPECTRA; FUNCTIONAL CHARACTERISTICS; FUNCTIONAL CHARACTERIZATION; HUMAN SERUM TRANSFERRIN; IRON BINDING; IRON IONS; IRON TRANSPORT; MALDI TOF MS; MATRIX ASSISTED LASER DESORPTION/IONIZATION; N-ACETYLGLUCOSAMINIDASE; N-TERMINALS; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; RECOMBINANT PROTEIN; SECONDARY STRUCTURES; SEQUENCE ANALYSIS; SIGNAL SEQUENCE; TARGET CELLS; TITRATION ANALYSIS; TRANSFERRIN RECEPTORS; YEAST SACCHAROMYCES CEREVISIAE;

BINDING SITES; BODY FLUIDS; CARBOHYDRATES; CELL CULTURE; DESORPTION; DICHROISM; ELECTROPHORESIS; MASS SPECTROMETRY; METAL IONS; TITRATION; UREA; YEAST;

METAL ANALYSIS;

CARBOHYDRATE; IRON; RECOMBINANT PROTEIN; TRANSFERRIN;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CHEMISTRY; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; PICHIA; PROTEIN BINDING; PROTEIN SECONDARY STRUCTURE;

AMINO ACID SEQUENCE; BINDING SITES; CARBOHYDRATES; HUMANS; IRON; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PICHIA; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; TRANSFERRIN;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 77249172664     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.90635     Document Type: Article

References (32)

1. Aisen P and Listowsky i, Annu. Rev. Biochem., 49, 357-393 (1980).
2. Sharma AK and Singh TP, Acta Crystallogr. D Biol. Crystallogr., 55, 1799-1804 (1999).
3. Karthikeyan S, Yadav S, Paramasivam M, Srinivasan A, and Singh TP, Acta Crystallogr. D Biol. Crystallogr., 56, 684-689 (2000).
4. Bailey S, Evans RW, Garratt RC, Gorinsky B, Hasnain S, Horsburgh C, Jhoti H, Lindley PF, Mydin A, Sarra R, and Watson JL, Biochemistry, 27, 5804-5812 (1988).
5. Hall DR, Hadden JM, Leonard GA, Bailey S, Neu M, Winn M, and Lindley PF, Acta Crystallogr. D Biol. Crystallogr., 58, 70-80 (2002).
6. Kurokawa H, Mikami B, and Hirose M, J. Mol. Biol., 254, 196-207 (1995).
7. Rawas A, Muirhead H, and Williams J, Acta Crystallogr. D Biol. Crystallogr., 52, 631-640 (1996).
8. Wally J, Halbrooks PJ, Vonrhein C, Rould MA, Everse SJ, Mason AB, and Buchanan SK, J. Biol. Chem., 281, 24934-24944 (2006).
9. Anderson BF, Baker HM, Norris GE, Rumball SV, and Baker EN, Nature, 344, 784-787 (1990).
10. Mizutani K, Yamashita H, Mikami B, and Hirose M, Biochemistry, 39, 3258-3265 (2000).
11. Mizutani K, Mikami B, and Hirose M, J. Mol. Biol., 309, 937-947 (2001).
12. Mizutani K, Yamashita H, Kurokawa H, Mikami B, and Hirose M, J. Biol. Chem., 274, 10190-10194 (1999).
13. Klausner RD, Ashwell G, van Renswoude J, Harford JB, and Bridges KR, Proc. Natl. Acad. Sci. USA, 80, 2263-2266 (1983).
14. Muralidhara BK and Hirose M, J. Biol. Chem., 275, 12463-12469 (2000).
15. Mizutani K, Muralidhara BK, Yamashita H, Tabata S, Mikami B, and Hirose M, J. Biol. Chem., 276, 35940-35946 (2001).
16. Egan TJ, Zak O, and Aisen P, Biochemistry, 32, 8162-8167 (1993).
17. Qian ZM, Li HY, Sun HZ, and Ho KP, Pharmacol. Rev., 54, 561-587 (2002).
18. Wiwanitkit V, Int. J. Mol. Sci., 7, 197-203 (2006).
19. Sakaguchi N, Kojima C, Harada A, Koiwai K, Emi N, and Kono K, Bioconj. Chem., 19, 1588-1595 (2008).
20. Steinlein LM, Graf TN, and ikeda RA, Protein Expr. Purif., 6, 619-624 (1995).
21. Mizutani K, Okamoto i, Fujita K, Yamamoto K, and Hirose M, Biosci. Biotechnol. Biochem., 68, 376-383 (2004).
22. Wang SH, Yang TS, Lin SM, Tsai MS, Wu SC, and Mao SJ, Protein Expr. Purif., 25, 41-49 (2002).
23. Paramasivam M, Saravanan K, Uma K, Sharma S, Singh TP, and Srinivasan A, Protein Expr. Purif., 26, 28-34 (2002).
24. Laemmli UK, Nature, 227, 680-685 (1970).
25. Burnette WN, Anal. Biochem., 112, 195-203 (1981).
26. Hirose M, Akuta T, and Takahashi N, J. Biol. Chem., 264, 16867-16872 (1989).
27. Mason AB, Halbrooks PJ, Larouche JR, Briggs SK, Moffett ML, Ramsey JE, Connolly SA, Smith VC, and MacGillivray RT, Protein Expr. Purif., 36, 318-326 (2004).
28. Miyauchi T, Takahashi N, and Hirose M, Biosci. Biotechnol. Biochem., 61, 2125-2126 (1997).
29. White CE, Kempi NM, and Komives EA, Structure, 2, 1003-005 (1994).
30. Letourneur O, Gervasi G, Gaia S, Pages J, Watelet B, and Jolivet M, Biotechnol. Appl. Biochem., 33, 35-45 (2001).
31. Trimble RB, Atkinson PH, Tschopp JF, Townsend RR, and Maley F, J. Biol. Chem., 266, 22807-22817 (1991).
32. Lin LN, Mason AB, Woodworth RC, and Brandts JF, Biochemistry, 33, 1881-1888 (1994).