Rational mutagenesis of cyclodextrin glucanotransferase at the calcium binding regions for enhancement of thermostability

International Journal of Molecular Sciences

Volumn 13, Issue 5, 2012, Pages 5307-5323

  Goh, Poh Hong ^a   Md Illias, Rosli ^a   Goh, Kian Mau ^a  

^a UNIVERSITI TEKNOLOGI MALAYSIA   (Malaysia)

Author keywords

Calcium binding site; CGTase; Protein engineering; Site directed mutagenesis; Thermostable enzyme

Indexed keywords

CALCIUM ION; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; CALCIUM; GLUCOSYLTRANSFERASE;

ALPHA HELIX; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CALCIUM BINDING; CONTROLLED STUDY; CRYSTAL STRUCTURE; CYCLIZATION; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE GENE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME METABOLISM; ENZYME STABILITY; GENE; HALF LIFE TIME; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; BACILLUS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN CONFORMATION; TEMPERATURE;

BACILLUS SP.;

AMINO ACID SEQUENCE; BACILLUS; BINDING SITES; CALCIUM; ENZYME STABILITY; GLUCOSYLTRANSFERASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; TEMPERATURE;

EID: 84861653716     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms13055307     Document Type: Article

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