Role of the calcium-binding residues Asp231, Asp233, and Asp438 in alpha-amylase of bacillus amyloliquefaciens as revealed by mutational analysis

Current Microbiology

Volumn 60, Issue 3, 2010, Pages 162-166

  Liu, Yang ^a   Shen, Wei ^a   Shi, Gui Yang ^a   Wang, Zheng Xiang ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLASE; CALCIUM BINDING PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS AMYLOLIQUEFACIENS; BINDING SITE; CALCIUM BINDING; ENZYME ANALYSIS; GENE EXPRESSION; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

ALPHA-AMYLASES; AMINO ACID SUBSTITUTION; ASPARTIC ACID; BACILLUS; BINDING SITES; CALCIUM; CATALYSIS; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; THERMODYNAMICS;

BACILLUS AMYLOLIQUEFACIENS;

EID: 76949095649     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-009-9517-5     Document Type: Article

References (21)

1. A Pandey P Nigam CR Soccol VT Soccol D Singh R Mohan 2000 Advances in microbial amylases Biotechnol Appl Biochem 31 Pt 2 135 152 10.1042/BA19990073 1:CAS:528:DC%2BD3cXislWitr4%3D 10744959
2. IS Pretorius E Laing GH Pretorius J Marmur 1988 Expression of a Bacillus alpha-amylase gene in yeast Curr Genet 14 1 8 10.1007/BF00405846 1:CAS:528:DyaL1cXls1Cqsbg%3D 2843299
3. JR Cherry AL Fidantsef 2003 Directed evolution of industrial enzymes: an update Curr Opin Biotechnol 14 438 443 10.1016/S0958-1669(03)00099-5 1:CAS:528:DC%2BD3sXmslenu7s%3D 12943855 (Pubitemid 37011326)
4. JE Nielsen TV Borchert 2000 Protein engineering of bacterial alpha-amylases Biochim Biophys Acta 1543 253 274 1:CAS:528: DC%2BD3MXitl2ltA%3D%3D 11150610
5. S Janecek 1997 alpha-Amylase family: molecular biology and evolution Prog Biophys Mol Biol 67 67 97 10.1016/S0079-6107(97)00015-1 1:CAS:528: DyaK2sXnslajsL4%3D 9401418 (Pubitemid 27491749)
6. T Kuriki T Imanaka 1999 The concept of the alpha-amylase family: structural similarity and common catalytic mechanism J Biosci Bioeng 87 557 565 10.1016/S1389-1723(99)80114-5 1:CAS:528:DyaK1MXltFCmu74%3D 16232518 (Pubitemid 29315284)
7. A Tanaka E Hoshino 2003 Secondary calcium-binding parameter of Bacillus amyloliquefaciens alpha-amylase obtained from inhibition kinetics J Biosci Bioeng 96 262 267 1:CAS:528:DC%2BD3sXptVygsLY%3D 16233519
8. AM Brzozowski DM Lawson JP Turkenburg H Bisgaard-Frantzen A Svendsen TV Borchert Z Dauter KS Wilson GJ Davies 2000 Structural analysis of a chimeric bacterial alpha-amylase. High-resolution analysis of native and ligand complexes Biochemistry 39 9099 9107 10.1021/bi0000317 1:CAS:528:DC%2BD3cXksleisL4%3D 10924103 (Pubitemid 30626303)
9. S Lee Y Mouri M Minoda H Oneda K Inouye 2006 Comparison of the wild-type alpha-amylase and its variant enzymes in Bacillus amyloliquefaciens in activity and thermal stability, and insights into engineering the thermal stability of Bacillus alpha-amylase J Biochem 139 1007 1015 10.1093/jb/mvj107 1:CAS:528:DC%2BD28Xotlygu7Y%3D 16788051 (Pubitemid 44196877)
10. MI Voskuil GH Chambliss 1993 Rapid isolation and sequencing of purified plasmid DNA from Bacillus subtilis Appl Environ Microbiol 59 1138 1142 1:CAS:528:DyaK3sXit1KktL0%3D 8476287
11. TJ Gryczan D Dubnau 1978 Construction and properties of chimeric plasmids in Bacillus subtilis Proc Natl Acad Sci USA 75 1428 1432 10.1073/pnas.75.3.1428 1:CAS:528:DyaE1cXkt1SjtLo%3D 418412 (Pubitemid 8301570)
12. Dieffenbach CW, Dveksler GS (2004) PCR primer: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
13. WB Zhi QY Deng JG Song M Gu F Ouyang 2005 One-step purification of alpha-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography with aqueous polymer two-phase systems J Chromatogr A 1070 215 219 10.1016/j.chroma.2005.02.065 1:CAS:528:DC%2BD2MXivVaqsL4%3D 15861808 (Pubitemid 40417787)
14. U Laemmli 1970 Cleavage of structural proteins during assembly of head of bacteriophage T4 Nature 227 680 685 10.1038/227680a0 1:CAS:528: DC%2BD3MXlsFags7s%3D 5432063
15. MM Bradford 1976 A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254 10.1016/0003-2697(76)90527-3 1:CAS:528: DyaE28XksVehtrY%3D 942051
16. ZZ Xiao R Storms A Tsang 2006 A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities Anal Biochem 351 146 148 10.1016/j.ab.2006.01.036 1:CAS:528:DC%2BD28XisF2gtLw%3D 16500607
17. I Palva 1982 Molecular cloning of alpha-amylase gene from Bacillus amyloliquefaciens and its expression in B. subtilis Gene 19 81 87 10.1016/0378-1119(82)90191-3 1:CAS:528:DyaL38XmtV2mur0%3D 6183169 (Pubitemid 13235744)
18. R Priyadharshini P Gunasekaran 2007 Site-directed mutagenesis of the calcium-binding site of alpha-amylase of Bacillus licheniformis Biotechnol Lett 29 1493 1499 10.1007/s10529-007-9428-0 1:CAS:528:DC%2BD2sXptlyls7s%3D 17598074 (Pubitemid 47301368)
19. N Declerck M Machius G Wiegand R Huber C Gaillardin 2000 Probing structural determinants specifying high thermostability in Bacillus licheniformis alpha-amylase J Mol Biol 301 1041 1057 10.1006/jmbi.2000.4025 1:CAS:528:DC%2BD3cXmtVKlsrg%3D 10966804 (Pubitemid 30706825)
20. A Savchenko C Vieille S Kang JG Zeikus 2002 Pyrococcus furiosus alpha-amylase is stabilized by calcium and zinc Biochemistry 41 6193 6201 10.1021/bi012106s 1:CAS:528:DC%2BD38XivFCgsbo%3D 11994016 (Pubitemid 34498927)
21. JK Lim HS Lee YJ Kim SS Bae JH Jeon SG Kang JH Lee 2007 Critical factors to high thermostability of an alpha-amylase from hyperthermophilic archaeon Thermococcus onnurineus NA1 J Microbiol Biotechnol 17 1242 1248 1:CAS:528:DC%2BD2sXhtFWhsLnF 18051591