Molecular cloning and characterization of a novel γ-CGTase from alkalophilic Bacillus sp.

Applied Microbiology and Biotechnology

Volumn 70, Issue 2, 2006, Pages 193-201

  Hirano, Kyoko ^a   Ishihara, Takeo ^a   Ogasawara, Satoshi ^a   Maeda, Hiroshi ^a   Abe, Keietsu ^a   Nakajima, Tasuku ^a   Yamagata, Youhei ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; MATHEMATICAL MODELS; PROTEINS;

AMINO ACID SEQUENCE; GLUCANOTRANSFERASE (CGTASE); PROTEIN STRUCTURE MODEL;

ENZYME KINETICS;

AMINO ACID; ASPARTIC ACID DERIVATIVE; BETA CYCLODEXTRIN; BETA CYCLODEXTRIN GLUCANOTRANSFERASE; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; GAMMA CYCLODEXTRIN; GAMMA CYCLODEXTRIN GLUCANOTRANSFERASE; GLYCOSYLTRANSFERASE; UNCLASSIFIED DRUG; VALINE;

BACTERIUM;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BACILLUS CLARKII; BACILLUS SUBTILIS; COMPARATIVE STUDY; CULTURE MEDIUM; DEGRADATION; EGGERTHELLA LENTA; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN STRUCTURE;

AMINO ACID SEQUENCE; BACILLUS; BACILLUS SUBTILIS; BASE SEQUENCE; CLONING, MOLECULAR; GAMMA-CYCLODEXTRINS; GLUCOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA;

BACILLUS CLARKII; BACILLUS SP.; BACILLUS SUBTILIS; EGGERTHELLA LENTA;

EID: 33644556465     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0041-7     Document Type: Article

References (28)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1987) Current protocols in molecular biology. Greene and Wiley, New York
2. Biwer A, Antranikian G, Heinzle E (2002) Enzymatic production of cyclodextrins. Appl Microbiol Biotechnol 59:609-617
3. Carter P, Bedouelle H, Winter G (1985) Improved oligonucleotide site-directed mutagenesis using M13 vectors. Nucleic Acids Res 13:4431-4443
4. Chang S, Cohen SN (1979) High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet 168:111-115
5. Gold L, Pribnow D, Schneider T, Shinedling S, Singer BS, Stormo G (1981) Translation initiation in prokaryotes. Annu Rev Microbiol 35:365-403
6. Harata K, Haga K, Nakamura A, Aoyagi M, Yamane M (1996) X-ray structure of cyclodextrin glucano-transferase from alkalophilic Bacillus Sp. 1011. Comparison of two independent molecules at 1.8 Å resolution. Acta Crystallogr, D Biol Crystallogr 52:1136-1145
7. Kato K, Horikoshi K (1984) Colorimetric determination of gamma-cyclodextrin. Agric Biol Chem 56:1738-1740
8. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
9. Lawson CL, van Montfort R, Strokopytov B, Rozeboom HJ, Kalk KH, de Vries GE, Penninga D, Dijkhuizen L, Dijkstra BW (1994) Nucleotide sequence and X-ray structure of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 in a maltose-dependent crystal form. J Mol Biol 236:590-600
10. Matsudaira P (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262:10035-10038
11. Moran C, Lang N, LeGrice S, Lee G, Stephans M, Sonenshein A, Pero J, Losick R (1982) Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet 186:339-346
12. Nakamura A, Koide Y, Kawamura F, Horinouchi S, Uozumi T, Beppu T (1990) Construction of a Bacillus subtilis strain deficient in three proteases. Agric Biol Chem 54:1307-1309
13. Parsiegla G, Schmidt AK, Schulz GE (1998) Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the gamma-cyclodextrin production. Eur J Biochem 255:710-717
14. Qi Q, Zimmermann W (2005) Cyclodextrin glucanotransferase: from gene to applications. Appl Microbiol Biotechnol 66:475-485
15. Saito H, Miura K (1963) Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619-629
16. Schmid G (1991) Gammer-CGTase. US Patent 5,409,824
17. Schwede T, Kopp J, Guex N, Peitsch MC (2003) SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res 31:3381-3385
18. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76-85
19. Strokopytov B, Knegtel RM, Penninga D, Rozeboom HJ, Kalk KH, Dijkhuizen L, Dijkstra BW (1996) Structure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 angstrom resolution. Implications for product specificity. Biochemistry 35:4241-4249
20. Takada M, Nakagawa Y, Yamamoto M (2003) Biochemical and genetic analyses of a novel gamma-cyclodextrin glucanotransferase from an alkalophilic Bacillus clarkii 7364. J Biochem (Tokyo) 133:317-324
21. Takami H, Takaki Y, Nakasone K, Sakiyama T, Maeno G, Sasaki R, Hirama C, Fuji F, Masui N (1999) Genetic analysis of the chromosome of alkaliphilic Bacillus halodurans C-125. Extremophiles 3:227-233
22. Takano T, Fukuda M, Monma M, Kobayashi S, Kainuma K, Yamane K (1986) Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene. J Bacteriol 166:1118-1122
23. Uitdehaag JC, Mosi R, Kalk KH, van der Veen BA, Dijkhuizen L, Withers SG, Dijkstra BW (1999a) X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family. Nat Struct Biol 6:432-436
24. Uitdehaag JC, Kalk KH, van der Veen BA, Dijkhuizen L, Dijkstra BW (1999b) The cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a gamma-cyclodextrin-CGTase complex at 1.8-A resolution. J Biol Chem 274:34868-34876
25. Uitdehaag JC, van Alebeek GJ, van Der Veen BA, Dijkhuizen L, Dijkstra BW (2000) Structures of maltohexaose and maltoheptaose bound at the donor sites of cyclodextrin glycosyltransferase give insight into the mechanisms of transglycosylation activity and cyclodextrin size specificity. Biochemistry 39:7772-7780
26. van der Veen BA, Uitdehaag JC, Dijkstra BW, Dijkhuizen L (2000) Engineering of cyclodextrin glycosyltransferase reaction and product specificity. Biochim Biophys Acta 1543:336-360
27. Yamagata Y, Isshiki K, Ichishima E (1995a) Subtilisin Sendai from alkalophilic Bacillus sp.: molecular and enzymatic properties of the enzyme and molecular cloning and characterization of the gene, aprS. Enzyme Microb Technol 17:653-663
28. Yamagata Y, Sato T, Hanzawa S, Ichishima E (1995b) The structure of subtilisin ALP I from alkalophilic Bacillus sp. NKS-21. Curr Microbiol 30:201-209