Expression, purification and characterization of fourth FAS1 domain of TGFβIp-associated corneal dystrophic mutants

Protein Expression and Purification

Volumn 84, Issue 1, 2012, Pages 108-115

  Elavazhagan, Murugan ^a   Lakshminarayanan, Rajamani ^a   Zhou, Lei ^a,c   Ting, Lim Wei ^a   Tong, Louis ^a,b,c,d   Beuerman, Roger W ^a,b,c,d   Chaurasia, Shyam S ^a   Mehta, Jodhbir S ^a,b,c,d  

^a SINGAPORE EYE RESEARCH INSTITUTE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c SINGAPORE NATIONAL EYE CENTRE   (Singapore)

^d DUKE NUS MEDICAL SCHOOL   (Singapore)

Author keywords

Conformational stability; Fluorescence spectroscopy; Granular corneal dystrophies; Lattice corneal dystrophies; Protein aggregation; TGF Ip

Indexed keywords

BETAIG H3 PROTEIN; BETAIG-H3 PROTEIN; MULTIPROTEIN COMPLEX; RECOMBINANT PROTEIN; SCLEROPROTEIN; TRANSFORMING GROWTH FACTOR BETA;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CIRCULAR DICHROISM; COMPUTER SIMULATION; CONGENITAL CORNEA DYSTROPHY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; MISSENSE MUTATION; MOLECULAR CLONING; MOLECULAR GENETICS; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; SPECTROFLUOROMETRY; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, LIQUID; CIRCULAR DICHROISM; CLONING, MOLECULAR; COMPUTER SIMULATION; CORNEAL DYSTROPHIES, HEREDITARY; EXTRACELLULAR MATRIX PROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; MULTIPROTEIN COMPLEXES; MUTATION, MISSENSE; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; TANDEM MASS SPECTROMETRY; TRANSFORMING GROWTH FACTOR BETA;

EID: 84861398593     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.04.018     Document Type: Article

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