DyeCycle violet used for side population detection is a substrate of P-glycoprotein

Cytometry Part A

Volumn 81 A, Issue 6, 2012, Pages 517-522

  Boesch, Maximilian ^a   Reimer, Daniel ^a   Rumpold, Holger ^b   Zeimet, Alain G ^a   Sopper, Sieghart ^a   Wolf, Dominik ^a,c  

^a INNSBRUCK MEDICAL UNIVERSITY   (Austria)

^b Klinikum der Barmherzigen Schwestern Linz   (Austria)

^c UNIVERSITY HOSPITAL BONN   (Germany)

Author keywords

ABC drug transporter; Cancer stem cells; DyeCycle Violet; P glycoprotein; Side population sorting

Indexed keywords

BENZIMIDAZOLE DERIVATIVE; FLUORESCENT DYE; GLYCOPROTEIN P; HOE 33342; TUMOR PROTEIN;

ARTICLE; CANCER STEM CELL; CELL SEPARATION; CELL STRAIN K 562; FLOW CYTOMETRY; HUMAN; LABORATORY DIAGNOSIS; LASER; METABOLISM; METHODOLOGY; PATHOLOGY; TRANSPORT AT THE CELLULAR LEVEL; ULTRAVIOLET RADIATION;

BENZIMIDAZOLES; BIOLOGICAL TRANSPORT; CELL SEPARATION; FALSE POSITIVE REACTIONS; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; K562 CELLS; LASERS; NEOPLASM PROTEINS; NEOPLASTIC STEM CELLS; P-GLYCOPROTEIN; ULTRAVIOLET RAYS;

EID: 84861340867     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22038     Document Type: Article

References (27)

1. Dean M, Fojo T, Bates S. Tumour stem cells and drug resistance. Nat Rev Cancer 2005; 5: 275-284.
2. Li L, Bhatia R. Stem cell quiescence. Clin Cancer Res 2011; 17: 4936-4941.
3. Schatton T, Murphy GF, Frank NY, Yamaura K, Waaga-Gasser AM, Gasser M, Zhan Q, Jordan S, Duncan LM, Weishaupt C, et al. Identification of cells initiating human melanomas. Nature 2008; 451: 345-349.
4. Al-Hajj M, Wicha MS, Benito-Hernandez A, Morrison SJ, Clarke MF. Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci USA 2003; 100: 3983-3988.
5. Li C, Heidt DG, Dalerba P, Burant CF, Zhang L, Adsay V, Wicha M, Clarke MF, Simeone DM. Identification of pancreatic cancer stem cells. Cancer Res 2007; 67: 1030-1037.
6. Hu L, McArthur C, Jaffe RB. Ovarian cancer stem-like side-population cells are tumourigenic and chemoresistant. Br J Cancer 2010; 102: 1276-1283.
7. Zhou S, Schuetz JD, Bunting KD, Colapietro AM, Sampath J, Morris JJ, Lagutina I, Grosveld GC, Osawa M, Nakauchi H, et al. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med 2001; 7: 1028-1034.
8. Hirschmann-Jax C, Foster AE, Wulf GG, Nuchtern JG, Jax TW, Gobel U, Goodell MA, Brenner MK. A distinct "side population" of cells with high drug efflux capacity in human tumor cells. Proc Natl Acad Sci USA 2004; 101: 14228-14233.
9. Telford WG, Bradford J, Godfrey W, Robey RW, Bates SE. Side population analysis using a violet-excited cell-permeable DNA binding dye. Stem Cells 2007; 25: 1029-1036.
10. Mathew G, Timm EAJr, Sotomayor P, Godoy A, Montecinos VP, Smith GJ, Huss WJ. ABCG2-mediated DyeCycle Violet efflux defined side population in benign and malignant prostate. Cell Cycle 2009; 8: 1053-1061.
11. Goldstein LJ, Galski H, Fojo A, Willingham M, Lai SL, Gazdar A, Pirker R, Green A, Crist W, Brodeur GM, et al. Expression of a multidrug resistance gene in human cancers. J Natl Cancer Inst 1989; 81: 116-124.
12. Rumpold H, Wolf AM, Gruenewald K, Gastl G, Gunsilius E, Wolf D. RNAi-mediated knockdown of P-glycoprotein using a transposon-based vector system durably restores imatinib sensitivity in imatinib-resistant CML cell lines. Exp Hematol 2005; 33: 767-775.
13. Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med 1996; 183: 1797-1806.
14. Golebiewska A, Brons NH, Bjerkvig R, Niclou SP. Critical appraisal of the side population assay in stem cell and cancer stem cell research. Cell Stem Cell 2011; 8: 136-147.
15. Scharenberg CW, Harkey MA, Torok-Storb B. The ABCG2 transporter is an efficient Hoechst 33342 efflux pump and is preferentially expressed by immature human hematopoietic progenitors. Blood 2002; 99: 507-512.
16. Shapiro AB, Ling V. The mechanism of ATP-dependent multidrug transport by P-glycoprotein. Acta Physiol Scand Suppl 1998; 643: 227-234.
17. Clevers H. The cancer stem cell: Premises, promises and challenges. Nat Med 2011; 17: 313-319.
18. Salcido CD, Larochelle A, Taylor BJ, Dunbar CE, Varticovski L. Molecular characterisation of side population cells with cancer stem cell-like characteristics in small-cell lung cancer. Br J Cancer 2010; 102: 1636-1644.
19. Gottesman MM, Fojo T, Bates SE. Multidrug resistance in cancer: Role of ATP-dependent transporters. Nat Rev Cancer 2002; 2: 48-58.
20. Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G. MDR1 expression identifies human melanoma stem cells. Biochem Biophys Res Commun 2008; 368: 930-936.
21. Ho MM, Hogge DE, Ling V. MDR1 and BCRP1 expression in leukemic progenitors correlates with chemotherapy response in acute myeloid leukemia. Exp Hematol 2008; 36: 433-442.
22. Callaghan R, Crowley E, Potter S, Kerr ID. P-glycoprotein: So many ways to turn it on. J Clin Pharmacol 2008; 48: 365-378.
23. Chaudhary PM, Roninson IB. Induction of multidrug resistance in human cells by transient exposure to different chemotherapeutic drugs. J Natl Cancer Inst 1993; 85: 632-639.
24. Kohno K, Sato S, Takano H, Matsuo K, Kuwano M. The direct activation of human multidrug resistance gene (MDR1) by anticancer agents. Biochem Biophys Res Commun 1989; 165: 1415-1421.
25. Rumpold H, Salvador C, Wolf AM, Tilg H, Gastl G, Wolf D. Knockdown of PgP resensitizes leukemic cells to proteasome inhibitors. Biochem Biophys Res Commun 2007; 361: 549-554.
26. Ho MM, Ng AV, Lam S, Hung JY. Side population in human lung cancer cell lines and tumors is enriched with stem-like cancer cells. Cancer Res 2007; 67: 4827-4833.
27. Kim M, Turnquist H, Jackson J, Sgagias M, Yan Y, Gong M, Dean M, Sharp JG, Cowan K. The multidrug resistance transporter ABCG2 (breast cancer resistance protein 1) effluxes Hoechst 33342 and is overexpressed in hematopoietic stem cells. Clin Cancer Res 2002; 8: 22-28.