Human cytomegalovirus: Bacterial artificial chromosome (BAC) cloning and genetic manipulation

Current Protocols in Microbiology

Volumn , Issue SUPPL.24, 2012, Pages

  Paredes, Anne M ^a   Yu, Dong ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

BAC; Bacterial artificial chromosome; Betaherpesvirus; HCMV; Herpesvirus; Human cytomegalovirus; Mutagenesis; Virus reconstitution

Indexed keywords

AGAROSE; DEOXYGALACTOSE; FLIPPASE RECOMBINATION ENZYME; REAGENT; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; VIRUS DNA;

ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CLINICAL PROTOCOL; DNA DETERMINATION; DNA ISOLATION; ESCHERICHIA COLI; EXPERIMENTAL DESIGN; GENE AMPLIFICATION; GENE CASSETTE; GENE ISOLATION; GENE LOCUS; GENE VECTOR; GENETIC MANIPULATION; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; HUMAN CYTOMEGALOVIRUS; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PLASMID; PRIORITY JOURNAL; PROCESS DESIGN; PROCESS DEVELOPMENT; PROTEIN FUNCTION; PROTEIN STABILITY; VIRION; VIRUS GENOME; VIRUS RECOMBINANT; CELL CULTURE; CYTOMEGALOVIRUS; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETICS; METHODOLOGY; VIRUS CULTURE;

BACTERIA (MICROORGANISMS); EUKARYOTA; HERPESVIRIDAE; HUMAN HERPESVIRUS 5; PROKARYOTA;

CELLS, CULTURED; CHROMOSOMES, ARTIFICIAL, BACTERIAL; CYTOMEGALOVIRUS; ESCHERICHIA COLI; GENETIC ENGINEERING; GENETIC VECTORS; HUMANS; RECOMBINATION, GENETIC; VIRUS CULTIVATION;

EID: 84861322859     PISSN: 19348525     EISSN: 19348533     Source Type: Journal    
DOI: 10.1002/9780471729259.mc14e04s24     Document Type: Article

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